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| | + | '''Unreleased structure''' |
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| - | ==CryoEM structure of yeast recombination mediator Rad52==
| + | The entry 8g3g is ON HOLD until 2025-02-07 |
| - | <StructureSection load='8g3g' size='340' side='right'caption='[[8g3g]], [[Resolution|resolution]] 3.50Å' scene=''>
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| - | == Structural highlights ==
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| - | <table><tr><td colspan='2'>[[8g3g]] is a 10 chain structure with sequence from [https://en.wikipedia.org/wiki/Saccharomyces_cerevisiae_S288C Saccharomyces cerevisiae S288C]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=8G3G OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=8G3G FirstGlance]. <br>
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| - | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 3.5Å</td></tr>
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| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=8g3g FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=8g3g OCA], [https://pdbe.org/8g3g PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=8g3g RCSB], [https://www.ebi.ac.uk/pdbsum/8g3g PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=8g3g ProSAT]</span></td></tr>
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| - | </table>
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| - | == Function ==
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| - | [https://www.uniprot.org/uniprot/RAD52_YEAST RAD52_YEAST] Involved in DNA double-strand break (DSB) repair and recombination. Promotes the annealing of complementary single-stranded DNA and by stimulation of the RAD51 recombinase.
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| - | <div style="background-color:#fffaf0;">
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| - | == Publication Abstract from PubMed ==
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| - | Homologous recombination (HR) is an essential double-stranded DNA break repair pathway. In HR, Rad52 facilitates the formation of Rad51 nucleoprotein filaments on RPA-coated ssDNA. Here, we decipher how Rad52 functions using single-particle cryo-electron microscopy and biophysical approaches. We report that Rad52 is a homodecameric ring and each subunit possesses an ordered N-terminal and disordered C-terminal half. An intrinsic structural asymmetry is observed where a few of the C-terminal halves interact with the ordered ring. We describe two conserved charged patches in the C-terminal half that harbor Rad51 and RPA interacting motifs. Interactions between these patches regulate ssDNA binding. Surprisingly, Rad51 interacts with Rad52 at two different bindings sites: one within the positive patch in the disordered C-terminus and the other in the ordered ring. We propose that these features drive Rad51 nucleation onto a single position on the DNA to promote formation of uniform pre-synaptic Rad51 filaments in HR.
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| - | Yeast Rad52 is a homodecamer and possesses BRCA2-like bipartite Rad51 binding modes.,Deveryshetty J, Chadda R, Mattice JR, Karunakaran S, Rau MJ, Basore K, Pokhrel N, Englander N, Fitzpatrick JAJ, Bothner B, Antony E Nat Commun. 2023 Oct 5;14(1):6215. doi: 10.1038/s41467-023-41993-1. PMID:37798272<ref>PMID:37798272</ref>
| + | Authors: Deveryshetty, J., Basore, K., Rau, M., Fitzpatrick, J.A.J., Antony, E. |
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| - | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
| + | Description: CryoEM structure of yeast recombination mediator Rad52 |
| - | </div>
| + | [[Category: Unreleased Structures]] |
| - | <div class="pdbe-citations 8g3g" style="background-color:#fffaf0;"></div>
| + | [[Category: Basore, K]] |
| - | == References ==
| + | [[Category: Antony, E]] |
| - | <references/>
| + | [[Category: Fitzpatrick, J.A.J]] |
| - | __TOC__
| + | [[Category: Rau, M]] |
| - | </StructureSection>
| + | [[Category: Deveryshetty, J]] |
| - | [[Category: Large Structures]] | + | |
| - | [[Category: Saccharomyces cerevisiae S288C]] | + | |
| - | [[Category: Antony E]] | + | |
| - | [[Category: Basore K]] | + | |
| - | [[Category: Deveryshetty J]]
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| - | [[Category: Fitzpatrick JAJ]] | + | |
| - | [[Category: Rau M]] | + | |
