|
|
| Line 1: |
Line 1: |
| | + | '''Unreleased structure''' |
| | | | |
| - | ==Crystal structure of the murine astrovirus capsid spike at 1.75 A==
| + | The entry 8tn8 is ON HOLD until Paper Publication |
| - | <StructureSection load='8tn8' size='340' side='right'caption='[[8tn8]], [[Resolution|resolution]] 1.75Å' scene=''>
| + | |
| - | == Structural highlights ==
| + | |
| - | <table><tr><td colspan='2'>[[8tn8]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Murine_astrovirus Murine astrovirus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=8TN8 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=8TN8 FirstGlance]. <br>
| + | |
| - | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.75Å</td></tr>
| + | |
| - | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=K:POTASSIUM+ION'>K</scene></td></tr>
| + | |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=8tn8 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=8tn8 OCA], [https://pdbe.org/8tn8 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=8tn8 RCSB], [https://www.ebi.ac.uk/pdbsum/8tn8 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=8tn8 ProSAT]</span></td></tr>
| + | |
| - | </table>
| + | |
| - | == Function ==
| + | |
| - | [https://www.uniprot.org/uniprot/A0A482N9T7_9VIRU A0A482N9T7_9VIRU]
| + | |
| - | <div style="background-color:#fffaf0;">
| + | |
| - | == Publication Abstract from PubMed ==
| + | |
| - | Human astroviruses (HAstVs) are small, non-enveloped icosahedral RNA viruses that are a significant cause of diarrhoea in young children. Despite their worldwide prevalence, HAstV pathogenesis studies and vaccine development remain challenging due to the lack of an animal model for HAstV infection. The recent development of a murine astrovirus (MuAstV) infection model in mice provides the opportunity to test proof-of-concept vaccines based on MuAstV antigens. To help establish a system in which an astrovirus capsid spike-based vaccine could be tested in vivo, we designed and produced a recombinant MuAstV capsid spike protein based on predicted secondary structure homology to HAstV spike proteins. The recombinant MuAstV spike can be expressed with high efficiency in Escherichia coli and retains antigenicity to polyclonal antibodies elicited by MuAstV infection. We determined the crystal structure of the MuAstV spike to 1.75 A and assessed its structural conservation with HAstV capsid spike. Despite low sequence identity between the MuAstV and HAstV spikes and differences in their overall shapes, they share related structural folds. Additionally, we found that vaccination with MuAstV spike induced anti-MuAstV-spike antibodies, highlighting that the recombinant spike is immunogenic. These studies lay a foundation for future in vivo MuAstV challenge studies to test whether MuAstV spike can be the basis of an effective vaccine.
| + | |
| | | | |
| - | Structure and immunogenicity of the murine astrovirus capsid spike.,Lanning S, Pedicino N, Haley DJ, Hernandez S, Cortez V, DuBois RM J Gen Virol. 2023 Nov;104(11). doi: 10.1099/jgv.0.001913. PMID:37910165<ref>PMID:37910165</ref>
| + | Authors: |
| | | | |
| - | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
| + | Description: |
| - | </div>
| + | [[Category: Unreleased Structures]] |
| - | <div class="pdbe-citations 8tn8" style="background-color:#fffaf0;"></div>
| + | |
| - | == References ==
| + | |
| - | <references/>
| + | |
| - | __TOC__
| + | |
| - | </StructureSection>
| + | |
| - | [[Category: Large Structures]] | + | |
| - | [[Category: Murine astrovirus]]
| + | |
| - | [[Category: DuBois RM]]
| + | |
| - | [[Category: Lanning S]]
| + | |
