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| | <StructureSection load='5x2m' size='340' side='right'caption='[[5x2m]], [[Resolution|resolution]] 2.21Å' scene=''> | | <StructureSection load='5x2m' size='340' side='right'caption='[[5x2m]], [[Resolution|resolution]] 2.21Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[5x2m]] is a 8 chain structure with sequence from [http://en.wikipedia.org/wiki/Japanese_killifish Japanese killifish] and [http://en.wikipedia.org/wiki/Lk3_transgenic_mice Lk3 transgenic mice]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5X2M OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=5X2M FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[5x2m]] is a 8 chain structure with sequence from [https://en.wikipedia.org/wiki/Mus_musculus Mus musculus] and [https://en.wikipedia.org/wiki/Oryzias_latipes Oryzias latipes]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5X2M OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5X2M FirstGlance]. <br> |
| - | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=GLN:GLUTAMINE'>GLN</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.206Å</td></tr> |
| - | <tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[5x2n|5x2n]], [[5x2o|5x2o]], [[5x2p|5x2p]], [[5x2q|5x2q]]</td></tr>
| + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=GLN:GLUTAMINE'>GLN</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene></td></tr> |
| - | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">TAS1R2a ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=8090 Japanese killifish]), TAS1R3 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=8090 Japanese killifish])</td></tr>
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5x2m FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5x2m OCA], [https://pdbe.org/5x2m PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5x2m RCSB], [https://www.ebi.ac.uk/pdbsum/5x2m PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5x2m ProSAT]</span></td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=5x2m FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5x2m OCA], [http://pdbe.org/5x2m PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5x2m RCSB], [http://www.ebi.ac.uk/pdbsum/5x2m PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5x2m ProSAT]</span></td></tr> | + | |
| | </table> | | </table> |
| | + | == Function == |
| | + | [https://www.uniprot.org/uniprot/A0A173M0G2_ORYLA A0A173M0G2_ORYLA] |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| | == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
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| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Japanese killifish]] | |
| | [[Category: Large Structures]] | | [[Category: Large Structures]] |
| - | [[Category: Lk3 transgenic mice]] | + | [[Category: Mus musculus]] |
| - | [[Category: Atsumi, N]] | + | [[Category: Oryzias latipes]] |
| - | [[Category: Nuemket, N]] | + | [[Category: Atsumi N]] |
| - | [[Category: Yamashita, A]] | + | [[Category: Nuemket N]] |
| - | [[Category: Yasui, N]]
| + | [[Category: Yamashita A]] |
| - | [[Category: Amino acid]] | + | [[Category: Yasui N]] |
| - | [[Category: Ligand binding]] | + | |
| - | [[Category: Receptor]]
| + | |
| - | [[Category: Signaling protein-immune system complex]]
| + | |
| - | [[Category: Venus-flytrap domain]]
| + | |
| Structural highlights
5x2m is a 8 chain structure with sequence from Mus musculus and Oryzias latipes. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
| | Method: | X-ray diffraction, Resolution 2.206Å |
| Ligands: | , , , , |
| Resources: | FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT |
Function
A0A173M0G2_ORYLA
Publication Abstract from PubMed
The taste receptor type 1 (T1r) family perceives 'palatable' tastes. These receptors function as T1r2-T1r3 and T1r1-T1r3 heterodimers to recognize a wide array of sweet and umami (savory) tastes in sugars and amino acids. Nonetheless, it is unclear how diverse tastes are recognized by so few receptors. Here we present crystal structures of the extracellular ligand-binding domains (LBDs), the taste recognition regions of the fish T1r2-T1r3 heterodimer, bound to different amino acids. The ligand-binding pocket in T1r2LBD is rich in aromatic residues, spacious and accommodates hydrated percepts. Biophysical studies show that this binding site is characterized by a broad yet discriminating chemical recognition, contributing for the particular trait of taste perception. In contrast, the analogous pocket in T1r3LBD is occupied by a rather loosely bound amino acid, suggesting that the T1r3 has an auxiliary role. Overall, we provide a structural basis for understanding the chemical perception of taste receptors.
Structural basis for perception of diverse chemical substances by T1r taste receptors.,Nuemket N, Yasui N, Kusakabe Y, Nomura Y, Atsumi N, Akiyama S, Nango E, Kato Y, Kaneko MK, Takagi J, Hosotani M, Yamashita A Nat Commun. 2017 May 23;8:15530. doi: 10.1038/ncomms15530. PMID:28534491[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Nuemket N, Yasui N, Kusakabe Y, Nomura Y, Atsumi N, Akiyama S, Nango E, Kato Y, Kaneko MK, Takagi J, Hosotani M, Yamashita A. Structural basis for perception of diverse chemical substances by T1r taste receptors. Nat Commun. 2017 May 23;8:15530. doi: 10.1038/ncomms15530. PMID:28534491 doi:http://dx.doi.org/10.1038/ncomms15530
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