1ocq

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[[Image:1ocq.jpg|left|200px]]
[[Image:1ocq.jpg|left|200px]]
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{{Structure
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<!--
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|PDB= 1ocq |SIZE=350|CAPTION= <scene name='initialview01'>1ocq</scene>, resolution 1.08&Aring;
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The line below this paragraph, containing "STRUCTURE_1ocq", creates the "Structure Box" on the page.
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|SITE= <scene name='pdbsite=NUC:So4+Binding+Site+For+Chain+A'>NUC</scene>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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|LIGAND= <scene name='pdbligand=BGC:BETA-D-GLUCOSE'>BGC</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=IFM:5-HYDROXYMETHYL-3,4-DIHYDROXYPIPERIDINE'>IFM</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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|ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/Cellulase Cellulase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.4 3.2.1.4] </span>
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or leave the SCENE parameter empty for the default display.
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|GENE=
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-->
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|DOMAIN=
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{{STRUCTURE_1ocq| PDB=1ocq | SCENE= }}
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|RELATEDENTRY=
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|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1ocq FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ocq OCA], [http://www.ebi.ac.uk/pdbsum/1ocq PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1ocq RCSB]</span>
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}}
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'''COMPLEX OF THE ENDOGLUCANASE CEL5A FROM BACILLUS AGARADHEARANS AT 1.08 ANGSTROM RESOLUTION WITH CELLOBIO-DERIVED ISOFAGOMINE'''
'''COMPLEX OF THE ENDOGLUCANASE CEL5A FROM BACILLUS AGARADHEARANS AT 1.08 ANGSTROM RESOLUTION WITH CELLOBIO-DERIVED ISOFAGOMINE'''
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[[Category: Varrot, A.]]
[[Category: Varrot, A.]]
[[Category: Withers, S G.]]
[[Category: Withers, S G.]]
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[[Category: cellulose degradation]]
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[[Category: Cellulose degradation]]
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[[Category: endoglucanase]]
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[[Category: Endoglucanase]]
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[[Category: glycosidase]]
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[[Category: Glycosidase]]
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[[Category: hydrolase]]
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[[Category: Hydrolase]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 03:40:26 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 22:43:18 2008''
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Revision as of 00:40, 3 May 2008

Image:1ocq.jpg

Template:STRUCTURE 1ocq

COMPLEX OF THE ENDOGLUCANASE CEL5A FROM BACILLUS AGARADHEARANS AT 1.08 ANGSTROM RESOLUTION WITH CELLOBIO-DERIVED ISOFAGOMINE


Overview

Glycosidases are some of the most ubiquitous enzyme in nature. Their biological significance, coupled to their enormous catalytic prowess derived from tight binding of the transition state, is reflected in their importance as therapeutic targets. Many glycosidase inhibitors are known. Imino sugars are often potent inhibitors, yet many facets of their mode of action, such as their degree, if any, of transition-state "mimicry" and their protonation state when bound to the target glycosidase remain unclear. Atomic resolution analysis of the endoglucanase, Cel5A, in complex with a cellobio-derived isofagomine in conjunction with the pH dependence of Ki and kcat/KM reveals that this compound binds as a protonated sugar. Surprisingly, both the enzymatic nucleophile and the acid/base are unprotonated in the complex.

About this Structure

1OCQ is a Single protein structure of sequence from Bacillus agaradhaerens. Full crystallographic information is available from OCA.

Reference

Direct observation of the protonation state of an imino sugar glycosidase inhibitor upon binding., Varrot A, Tarling CA, Macdonald JM, Stick RV, Zechel DL, Withers SG, Davies GJ, J Am Chem Soc. 2003 Jun 25;125(25):7496-7. PMID:12812472 Page seeded by OCA on Sat May 3 03:40:26 2008

Proteopedia Page Contributors and Editors (what is this?)

OCA

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