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5xaq

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Crystal structure of Animalia-specific tRNA deacylase from Mus musculus

Structural highlights

5xaq is a 2 chain structure with sequence from Mus musculus. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:	X-ray diffraction, Resolution 1.86Å
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

DTD2_MOUSE May hydrolyze D-tyrosyl-tRNA(Tyr) into D-tyrosine and free tRNA(Tyr). Could be a defense mechanism against a harmful effect of D-tyrosine (Potential).

Publication Abstract from PubMed

D-aminoacyl-tRNA deacylase (DTD), a bacterial/eukaryotic trans-editing factor, removes D-amino acids mischarged on tRNAs and achiral glycine mischarged on tRNA(Ala). An invariant cross-subunit Gly-cisPro motif forms the mechanistic basis of L-amino acid rejection from the catalytic site. Here, we present the identification of a DTD variant, named ATD (Animalia-specific tRNA deacylase), that harbors a Gly-transPro motif. The cis-to-trans switch causes a "gain of function" through L-chiral selectivity in ATD resulting in the clearing of L-alanine mischarged on tRNA(Thr)(G4*U69) by eukaryotic AlaRS. The proofreading activity of ATD is conserved across diverse classes of phylum Chordata. Animalia genomes enriched in tRNA(Thr)(G4*U69) genes are in strict association with the presence of ATD, underlining the mandatory requirement of a dedicated factor to proofread tRNA misaminoacylation. The study highlights the emergence of ATD during genome expansion as a key event associated with the evolution of Animalia.

A chiral selectivity relaxed paralog of DTD for proofreading tRNA mischarging in Animalia.,Kuncha SK, Mazeed M, Singh R, Kattula B, Routh SB, Sankaranarayanan R Nat Commun. 2018 Feb 6;9(1):511. doi: 10.1038/s41467-017-02204-w. PMID:29410408^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Kuncha SK, Mazeed M, Singh R, Kattula B, Routh SB, Sankaranarayanan R. A chiral selectivity relaxed paralog of DTD for proofreading tRNA mischarging in Animalia. Nat Commun. 2018 Feb 6;9(1):511. doi: 10.1038/s41467-017-02204-w. PMID:29410408 doi:http://dx.doi.org/10.1038/s41467-017-02204-w

1 Structural highlights
2 Function
3 Publication Abstract from PubMed
4 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/5xaq"

Categories: Large Structures | Mus musculus | Kattula B | Kuncha KS | Sankarnarayanan R

@@ Line 1: / Line 1: @@
 ==Crystal structure of Animalia-specific tRNA deacylase from Mus musculus==
-<StructureSection load='5xaq' size='340' side='right' caption='[[5xaq]], [[Resolution|resolution]] 1.86&Aring;' scene=''>
+<StructureSection load='5xaq' size='340' side='right'caption='[[5xaq]], [[Resolution|resolution]] 1.86&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[5xaq]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Lk3_transgenic_mice Lk3 transgenic mice]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5XAQ OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5XAQ FirstGlance]. <br>
+<table><tr><td colspan='2'>[[5xaq]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5XAQ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5XAQ FirstGlance]. <br>
-</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">Dtd2 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10090 LK3 transgenic mice])</td></tr>
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.86&#8491;</td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5xaq FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5xaq OCA], [http://pdbe.org/5xaq PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5xaq RCSB], [http://www.ebi.ac.uk/pdbsum/5xaq PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5xaq ProSAT]</span></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5xaq FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5xaq OCA], [https://pdbe.org/5xaq PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5xaq RCSB], [https://www.ebi.ac.uk/pdbsum/5xaq PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5xaq ProSAT]</span></td></tr>
 </table>
 == Function ==
-[[http://www.uniprot.org/uniprot/DTD2_MOUSE DTD2_MOUSE]] May hydrolyze D-tyrosyl-tRNA(Tyr) into D-tyrosine and free tRNA(Tyr). Could be a defense mechanism against a harmful effect of D-tyrosine (Potential).
+[https://www.uniprot.org/uniprot/DTD2_MOUSE DTD2_MOUSE] May hydrolyze D-tyrosyl-tRNA(Tyr) into D-tyrosine and free tRNA(Tyr). Could be a defense mechanism against a harmful effect of D-tyrosine (Potential).
 <div style="background-color:#fffaf0;">
 == Publication Abstract from PubMed ==
@@ Line 22: / Line 22: @@
 __TOC__
 </StructureSection>
-[[Category: Lk3 transgenic mice]]
+[[Category: Large Structures]]
-[[Category: Kattula, B]]
+[[Category: Mus musculus]]
-[[Category: Kuncha, K S]]
+[[Category: Kattula B]]
-[[Category: Sankarnarayanan, R]]
+[[Category: Kuncha KS]]
-[[Category: Deacylase]]
+[[Category: Sankarnarayanan R]]
-[[Category: Hydrolase]]
-[[Category: Trna]]

5xaq

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Current revision

Crystal structure of Animalia-specific tRNA deacylase from Mus musculus

Structural highlights

Function

Publication Abstract from PubMed

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

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