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5xrz

From Proteopedia

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Structure of a ssDNA bound to the inner DNA binding site of RAD52

Structural highlights

5xrz is a 12 chain structure with sequence from Homo sapiens and Synthetic construct. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:	X-ray diffraction, Resolution 3.6Å
Ligands:
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

RAD52_HUMAN Involved in double-stranded break repair. Plays a central role in genetic recombination and DNA repair by promoting the annealing of complementary single-stranded DNA and by stimulation of the RAD51 recombinase.^[1]

Publication Abstract from PubMed

RAD52 mediates homologous recombination by annealing cDNA strands. However, the detailed mechanism of DNA annealing promoted by RAD52 has remained elusive. Here we report two crystal structures of human RAD52 single-stranded DNA (ssDNA) complexes that probably represent key reaction intermediates of RAD52-mediated DNA annealing. The first structure revealed a "wrapped" conformation of ssDNA around the homo-oligomeric RAD52 ring, in which the edges of the bases involved in base pairing are exposed to the solvent. The ssDNA conformation is close to B-form and appears capable of engaging in Watson-Crick base pairing with the cDNA strand. The second structure revealed a "trapped" conformation of ssDNA between two RAD52 rings. This conformation is stabilized by a different RAD52 DNA binding site, which promotes the accumulation of multiple RAD52 rings on ssDNA and the aggregation of ssDNA. These structures provide a structural framework for understanding the mechanism of RAD52-mediated DNA annealing.

Structural Basis of Homology-Directed DNA Repair Mediated by RAD52.,Saotome M, Saito K, Yasuda T, Ohtomo H, Sugiyama S, Nishimura Y, Kurumizaka H, Kagawa W iScience. 2018 May 25;3:50-62. doi: 10.1016/j.isci.2018.04.005. Epub 2018 Apr 12. PMID:30428330^[2]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Kitao H, Yuan ZM. Regulation of ionizing radiation-induced Rad52 nuclear foci formation by c-Abl-mediated phosphorylation. J Biol Chem. 2002 Dec 13;277(50):48944-8. Epub 2002 Oct 11. PMID:12379650 doi:10.1074/jbc.M208151200
↑ Saotome M, Saito K, Yasuda T, Ohtomo H, Sugiyama S, Nishimura Y, Kurumizaka H, Kagawa W. Structural Basis of Homology-Directed DNA Repair Mediated by RAD52. iScience. 2018 May 25;3:50-62. doi: 10.1016/j.isci.2018.04.005. Epub 2018 Apr 12. PMID:30428330 doi:http://dx.doi.org/10.1016/j.isci.2018.04.005

1 Structural highlights
2 Function
3 Publication Abstract from PubMed
4 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/5xrz"

@@ Line 1: / Line 1: @@
 ==Structure of a ssDNA bound to the inner DNA binding site of RAD52==
-<StructureSection load='5xrz' size='340' side='right' caption='[[5xrz]], [[Resolution|resolution]] 3.60&Aring;' scene=''>
+<StructureSection load='5xrz' size='340' side='right'caption='[[5xrz]], [[Resolution|resolution]] 3.60&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[5xrz]] is a 12 chain structure with sequence from [http://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5XRZ OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5XRZ FirstGlance]. <br>
+<table><tr><td colspan='2'>[[5xrz]] is a 12 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] and [https://en.wikipedia.org/wiki/Synthetic_construct Synthetic construct]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5XRZ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5XRZ FirstGlance]. <br>
-</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=K:POTASSIUM+ION'>K</scene></td></tr>
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 3.6&#8491;</td></tr>
-<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">RAD52 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])</td></tr>
+<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=K:POTASSIUM+ION'>K</scene></td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5xrz FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5xrz OCA], [http://pdbe.org/5xrz PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5xrz RCSB], [http://www.ebi.ac.uk/pdbsum/5xrz PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5xrz ProSAT]</span></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5xrz FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5xrz OCA], [https://pdbe.org/5xrz PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5xrz RCSB], [https://www.ebi.ac.uk/pdbsum/5xrz PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5xrz ProSAT]</span></td></tr>
 </table>
 == Function ==
-[[http://www.uniprot.org/uniprot/RAD52_HUMAN RAD52_HUMAN]] Involved in double-stranded break repair. Plays a central role in genetic recombination and DNA repair by promoting the annealing of complementary single-stranded DNA and by stimulation of the RAD51 recombinase.<ref>PMID:12379650</ref>
+[https://www.uniprot.org/uniprot/RAD52_HUMAN RAD52_HUMAN] Involved in double-stranded break repair. Plays a central role in genetic recombination and DNA repair by promoting the annealing of complementary single-stranded DNA and by stimulation of the RAD51 recombinase.<ref>PMID:12379650</ref>
 <div style="background-color:#fffaf0;">
 == Publication Abstract from PubMed ==
@@ Line 23: / Line 23: @@
 __TOC__
 </StructureSection>
-[[Category: Human]]
+[[Category: Homo sapiens]]
-[[Category: Kagawa, W]]
+[[Category: Large Structures]]
-[[Category: Kurumizaka, H]]
+[[Category: Synthetic construct]]
-[[Category: Saito, K]]
+[[Category: Kagawa W]]
-[[Category: Saotome, M]]
+[[Category: Kurumizaka H]]
-[[Category: Sugiyama, S]]
+[[Category: Saito K]]
-[[Category: Yasuda, T]]
+[[Category: Saotome M]]
-[[Category: Dna repair protein]]
+[[Category: Sugiyama S]]
-[[Category: Protein-dna complex]]
+[[Category: Yasuda T]]
-[[Category: Recombination]]
-[[Category: Ssdna annealing protein]]

5xrz

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Current revision

Structure of a ssDNA bound to the inner DNA binding site of RAD52

Structural highlights

Function

Publication Abstract from PubMed

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

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