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| | <StructureSection load='5xub' size='340' side='right'caption='[[5xub]], [[Resolution|resolution]] 2.50Å' scene=''> | | <StructureSection load='5xub' size='340' side='right'caption='[[5xub]], [[Resolution|resolution]] 2.50Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[5xub]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Comt2 Comt2]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5XUB OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5XUB FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[5xub]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Comamonas_testosteroni_CNB-2 Comamonas testosteroni CNB-2]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5XUB OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5XUB FirstGlance]. <br> |
| - | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CIT:CITRIC+ACID'>CIT</scene></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.5Å</td></tr> |
| - | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">CtCNB1_2201 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=688245 COMT2])</td></tr> | + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CIT:CITRIC+ACID'>CIT</scene></td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5xub FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5xub OCA], [http://pdbe.org/5xub PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5xub RCSB], [http://www.ebi.ac.uk/pdbsum/5xub PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5xub ProSAT]</span></td></tr> | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5xub FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5xub OCA], [https://pdbe.org/5xub PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5xub RCSB], [https://www.ebi.ac.uk/pdbsum/5xub PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5xub ProSAT]</span></td></tr> |
| | </table> | | </table> |
| | + | == Function == |
| | + | [https://www.uniprot.org/uniprot/D0IVL9_COMT2 D0IVL9_COMT2] |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| | == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
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| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Comt2]] | + | [[Category: Comamonas testosteroni CNB-2]] |
| | [[Category: Large Structures]] | | [[Category: Large Structures]] |
| - | [[Category: Hong, Y]] | + | [[Category: Hong Y]] |
| - | [[Category: Li, D F]] | + | [[Category: Li DF]] |
| - | [[Category: Wang, D C]] | + | [[Category: Wang DC]] |
| - | [[Category: Dicarboxylic organic acid binding]]
| + | |
| - | [[Category: Four helix bundle]]
| + | |
| - | [[Category: Methyl-accepting chemotaxis protein]]
| + | |
| - | [[Category: Signaling protein]]
| + | |
| Structural highlights
Function
D0IVL9_COMT2
Publication Abstract from PubMed
Transmembrane chemoreceptors are widely present in Bacteria and Archaea. They play a critical role in sensing various signals outside and transmitting to the cell interior. Here, we report the structure of the periplasmic ligand-binding domain (LBD) of the transmembrane chemoreceptor MCP2201, which governs chemotaxis to citrate and other organic compounds in Comamonas testosteroni. The apo-form LBD crystal revealed a typical four-helix bundle homodimer, similar to previously well-studied chemoreceptors such as Tar and Tsr of Escherichia coli. However, the citrate-bound LBD revealed a four-helix bundle homotrimer that had not been observed in bacterial chemoreceptor LBDs. This homotrimer was further confirmed with size-exclusion chromatography, analytical ultracentrifugation and cross-linking experiments. The physiological importance of the homotrimer for chemotaxis was demonstrated with site-directed mutations of key amino acid residues in C. testosteroni mutants.
The ligand-binding domain of a chemoreceptor from Comamonas testosteroni has a previously unknown homotrimeric structure.,Hong Y, Huang Z, Guo L, Ni B, Jiang CY, Li XJ, Hou YJ, Yang WS, Wang DC, Zhulin IB, Liu SJ, Li DF Mol Microbiol. 2019 Jun 8. doi: 10.1111/mmi.14326. PMID:31177588[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Hong Y, Huang Z, Guo L, Ni B, Jiang CY, Li XJ, Hou YJ, Yang WS, Wang DC, Zhulin IB, Liu SJ, Li DF. The ligand-binding domain of a chemoreceptor from Comamonas testosteroni has a previously unknown homotrimeric structure. Mol Microbiol. 2019 Jun 8. doi: 10.1111/mmi.14326. PMID:31177588 doi:http://dx.doi.org/10.1111/mmi.14326
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