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| | ==Crystal structure of Y145F mutant of KacT== | | ==Crystal structure of Y145F mutant of KacT== |
| - | <StructureSection load='5xun' size='340' side='right' caption='[[5xun]], [[Resolution|resolution]] 2.00Å' scene=''> | + | <StructureSection load='5xun' size='340' side='right'caption='[[5xun]], [[Resolution|resolution]] 2.00Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[5xun]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/"bacillus_pneumoniae"_(schroeter_1886)_flugge_1886 "bacillus pneumoniae" (schroeter 1886) flugge 1886]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5XUN OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5XUN FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[5xun]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Klebsiella_pneumoniae Klebsiella pneumoniae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5XUN OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5XUN FirstGlance]. <br> |
| - | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ACO:ACETYL+COENZYME+*A'>ACO</scene>, <scene name='pdbligand=ACT:ACETATE+ION'>ACT</scene></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2Å</td></tr> |
| - | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">AGG09_10975, BB749_24230, BL127_018840, BL143_022465 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=573 "Bacillus pneumoniae" (Schroeter 1886) Flugge 1886])</td></tr> | + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ACO:ACETYL+COENZYME+*A'>ACO</scene>, <scene name='pdbligand=ACT:ACETATE+ION'>ACT</scene></td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5xun FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5xun OCA], [http://pdbe.org/5xun PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5xun RCSB], [http://www.ebi.ac.uk/pdbsum/5xun PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5xun ProSAT]</span></td></tr> | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5xun FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5xun OCA], [https://pdbe.org/5xun PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5xun RCSB], [https://www.ebi.ac.uk/pdbsum/5xun PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5xun ProSAT]</span></td></tr> |
| | </table> | | </table> |
| | + | == Function == |
| | + | [https://www.uniprot.org/uniprot/A6THQ8_KLEP7 A6THQ8_KLEP7] |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| | == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
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| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Gan, J H]] | |
| - | [[Category: Ou, H Y]] | |
| - | [[Category: Qian, H L]] | |
| - | [[Category: Yao, Q Q]] | |
| | [[Category: Klebsiella pneumoniae]] | | [[Category: Klebsiella pneumoniae]] |
| - | [[Category: Toxin]] | + | [[Category: Large Structures]] |
| | + | [[Category: Gan JH]] |
| | + | [[Category: Ou HY]] |
| | + | [[Category: Qian HL]] |
| | + | [[Category: Yao QQ]] |
| Structural highlights
Function
A6THQ8_KLEP7
Publication Abstract from PubMed
A type II toxin-antitoxin (TA) system, in which the toxin contains a Gcn5-related N-acetyltransferase (GNAT) domain, has been characterized recently. GNAT toxin acetylates aminoacyl-tRNA and blocks protein translation. It is abolished by the cognate antitoxin that contains the ribbon-helix-helix (RHH) domain. Here, we present an experimental demonstration of the interaction of the GNAT-RHH complex with TA promoter DNA. First, the GNAT-RHH TA locus kacAT was found in Klebsiella pneumoniae HS11286, a strain resistant to multiple antibiotics. Overexpression of KacT halted cell growth and resulted in persister cell formation. The crystal structure also indicated that KacT is a typical acetyltransferase toxin. Co-expression of KacA neutralized KacT toxicity. Expression of the bicistronic kacAT locus was up-regulated during antibiotic stress. Finally, KacT and KacA formed a heterohexamer that interacted with promoter DNA, resulting in negative autoregulation of kacAT transcription. The N-terminus region of KacA accounted for specific binding to the palindromic sequence on the operator DNA, whereas its C-terminus region was essential for the inactivation of the GNAT toxin. These results provide an important insight into the regulation of the GNAT-RHH family TA system.
Identification and characterization of acetyltransferase-type toxin-antitoxin locus in Klebsiella pneumoniae.,Qian H, Yao Q, Tai C, Deng Z, Gan J, Ou HY Mol Microbiol. 2018 May;108(4):336-349. doi: 10.1111/mmi.13934. Epub 2018 Mar 8. PMID:29461656[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Qian H, Yao Q, Tai C, Deng Z, Gan J, Ou HY. Identification and characterization of acetyltransferase-type toxin-antitoxin locus in Klebsiella pneumoniae. Mol Microbiol. 2018 May;108(4):336-349. doi: 10.1111/mmi.13934. Epub 2018 Mar 8. PMID:29461656 doi:http://dx.doi.org/10.1111/mmi.13934
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