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| | ==Crystal structure of TZAP and telomeric DNA complex== | | ==Crystal structure of TZAP and telomeric DNA complex== |
| - | <StructureSection load='5yj3' size='340' side='right' caption='[[5yj3]], [[Resolution|resolution]] 2.84Å' scene=''> | + | <StructureSection load='5yj3' size='340' side='right'caption='[[5yj3]], [[Resolution|resolution]] 2.84Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[5yj3]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5YJ3 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5YJ3 FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[5yj3]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] and [https://en.wikipedia.org/wiki/Synthetic_construct Synthetic construct]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5YJ3 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5YJ3 FirstGlance]. <br> |
| - | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.845Å</td></tr> |
| - | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">ZBTB48, HKR3, TZAP, ZNF855 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])</td></tr> | + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5yj3 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5yj3 OCA], [http://pdbe.org/5yj3 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5yj3 RCSB], [http://www.ebi.ac.uk/pdbsum/5yj3 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5yj3 ProSAT]</span></td></tr> | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5yj3 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5yj3 OCA], [https://pdbe.org/5yj3 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5yj3 RCSB], [https://www.ebi.ac.uk/pdbsum/5yj3 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5yj3 ProSAT]</span></td></tr> |
| | </table> | | </table> |
| | == Function == | | == Function == |
| - | [[http://www.uniprot.org/uniprot/TZAP_HUMAN TZAP_HUMAN]] Telomere-binding protein that acts as a regulator of telomere length (PubMed:28500257, PubMed:28082411). Directly binds the telomeric double-stranded 5'-TTAGGG-3' repeat (PubMed:28500257, PubMed:28082411). Preferentially binds to telomeres that have a low concentration of shelterin complex and acts as a regulator of telomere length by initiating telomere trimming, a process that prevents the accumulation of aberrantly long telomeres (PubMed:28082411). Also acts as a transcription regulator that binds to promoter regions (PubMed:7969177, PubMed:24382891, PubMed:28500257). Regulates expression of a small subset of genes, including MTFP1 (PubMed:28500257). Regulates expression the J and/or S elements in MHC II promoter (PubMed:7969177). Acts as a negative regulator of cell proliferation by specifically activating expression of ARF, a tumor suppressor isoform of CDKN2A (PubMed:24382891).<ref>PMID:24382891</ref> <ref>PMID:28082411</ref> <ref>PMID:28500257</ref> <ref>PMID:7969177</ref> | + | [https://www.uniprot.org/uniprot/TZAP_HUMAN TZAP_HUMAN] Telomere-binding protein that acts as a regulator of telomere length (PubMed:28500257, PubMed:28082411). Directly binds the telomeric double-stranded 5'-TTAGGG-3' repeat (PubMed:28500257, PubMed:28082411). Preferentially binds to telomeres that have a low concentration of shelterin complex and acts as a regulator of telomere length by initiating telomere trimming, a process that prevents the accumulation of aberrantly long telomeres (PubMed:28082411). Also acts as a transcription regulator that binds to promoter regions (PubMed:7969177, PubMed:24382891, PubMed:28500257). Regulates expression of a small subset of genes, including MTFP1 (PubMed:28500257). Regulates expression the J and/or S elements in MHC II promoter (PubMed:7969177). Acts as a negative regulator of cell proliferation by specifically activating expression of ARF, a tumor suppressor isoform of CDKN2A (PubMed:24382891).<ref>PMID:24382891</ref> <ref>PMID:28082411</ref> <ref>PMID:28500257</ref> <ref>PMID:7969177</ref> |
| | == References == | | == References == |
| | <references/> | | <references/> |
| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Human]] | + | [[Category: Homo sapiens]] |
| - | [[Category: Li, F]] | + | [[Category: Large Structures]] |
| - | [[Category: Zhao, Y]] | + | [[Category: Synthetic construct]] |
| - | [[Category: Dna binding protein-dna complex]] | + | [[Category: Li F]] |
| - | [[Category: Telomeric dna binding protein]] | + | [[Category: Zhao Y]] |
| Structural highlights
Function
TZAP_HUMAN Telomere-binding protein that acts as a regulator of telomere length (PubMed:28500257, PubMed:28082411). Directly binds the telomeric double-stranded 5'-TTAGGG-3' repeat (PubMed:28500257, PubMed:28082411). Preferentially binds to telomeres that have a low concentration of shelterin complex and acts as a regulator of telomere length by initiating telomere trimming, a process that prevents the accumulation of aberrantly long telomeres (PubMed:28082411). Also acts as a transcription regulator that binds to promoter regions (PubMed:7969177, PubMed:24382891, PubMed:28500257). Regulates expression of a small subset of genes, including MTFP1 (PubMed:28500257). Regulates expression the J and/or S elements in MHC II promoter (PubMed:7969177). Acts as a negative regulator of cell proliferation by specifically activating expression of ARF, a tumor suppressor isoform of CDKN2A (PubMed:24382891).[1] [2] [3] [4]
References
- ↑ Yoon JH, Choi WI, Jeon BN, Koh DI, Kim MK, Kim MH, Kim J, Hur SS, Kim KS, Hur MW. Human Kruppel-related 3 (HKR3) is a novel transcription activator of alternate reading frame (ARF) gene. J Biol Chem. 2014 Feb 14;289(7):4018-31. doi: 10.1074/jbc.M113.526855. Epub 2014 , Jan 1. PMID:24382891 doi:http://dx.doi.org/10.1074/jbc.M113.526855
- ↑ Li JS, Miralles Fuste J, Simavorian T, Bartocci C, Tsai J, Karlseder J, Lazzerini Denchi E. TZAP: A telomere-associated protein involved in telomere length control. Science. 2017 Feb 10;355(6325):638-641. doi: 10.1126/science.aah6752. Epub 2017, Jan 12. PMID:28082411 doi:http://dx.doi.org/10.1126/science.aah6752
- ↑ Jahn A, Rane G, Paszkowski-Rogacz M, Sayols S, Bluhm A, Han CT, Draskovic I, Londono-Vallejo JA, Kumar AP, Buchholz F, Butter F, Kappei D. ZBTB48 is both a vertebrate telomere-binding protein and a transcriptional activator. EMBO Rep. 2017 Jun;18(6):929-946. doi: 10.15252/embr.201744095. Epub 2017 May 12. PMID:28500257 doi:http://dx.doi.org/10.15252/embr.201744095
- ↑ Sugawara M, Scholl T, Ponath PD, Strominger JL. A factor that regulates the class II major histocompatibility complex gene DPA is a member of a subfamily of zinc finger proteins that includes a Drosophila developmental control protein. Mol Cell Biol. 1994 Dec;14(12):8438-50. PMID:7969177
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