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| | ==Crystal structure of the PH-like domain of Lam6== | | ==Crystal structure of the PH-like domain of Lam6== |
| - | <StructureSection load='5yqr' size='340' side='right' caption='[[5yqr]], [[Resolution|resolution]] 2.40Å' scene=''> | + | <StructureSection load='5yqr' size='340' side='right'caption='[[5yqr]], [[Resolution|resolution]] 2.40Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[5yqr]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Bpt4 Bpt4]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5YQR OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5YQR FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[5yqr]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_virus_T4 Escherichia virus T4] and [https://en.wikipedia.org/wiki/Saccharomyces_cerevisiae_S288C Saccharomyces cerevisiae S288C]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5YQR OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5YQR FirstGlance]. <br> |
| - | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=2PE:NONAETHYLENE+GLYCOL'>2PE</scene></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.402Å</td></tr> |
| - | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">LAM6, LTC1, YLR072W ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10665 BPT4])</td></tr>
| + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=2PE:NONAETHYLENE+GLYCOL'>2PE</scene></td></tr> |
| - | <tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Lysozyme Lysozyme], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.17 3.2.1.17] </span></td></tr> | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5yqr FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5yqr OCA], [https://pdbe.org/5yqr PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5yqr RCSB], [https://www.ebi.ac.uk/pdbsum/5yqr PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5yqr ProSAT]</span></td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5yqr FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5yqr OCA], [http://pdbe.org/5yqr PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5yqr RCSB], [http://www.ebi.ac.uk/pdbsum/5yqr PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5yqr ProSAT]</span></td></tr> | + | |
| | </table> | | </table> |
| | == Function == | | == Function == |
| - | [[http://www.uniprot.org/uniprot/ENLYS_BPT4 ENLYS_BPT4]] Endolysin with lysozyme activity that degrades host peptidoglycans and participates with the holin and spanin proteins in the sequential events which lead to the programmed host cell lysis releasing the mature viral particles. Once the holin has permeabilized the host cell membrane, the endolysin can reach the periplasm and break down the peptidoglycan layer.<ref>PMID:22389108</ref> | + | [https://www.uniprot.org/uniprot/ENLYS_BPT4 ENLYS_BPT4] Endolysin with lysozyme activity that degrades host peptidoglycans and participates with the holin and spanin proteins in the sequential events which lead to the programmed host cell lysis releasing the mature viral particles. Once the holin has permeabilized the host cell membrane, the endolysin can reach the periplasm and break down the peptidoglycan layer.<ref>PMID:22389108</ref> [https://www.uniprot.org/uniprot/LAM6_YEAST LAM6_YEAST] Involved in regulation of various organellar membrane contact sites (PubMed:26119743). May be involved in sterol transfer between intracellular membranes (PubMed:26001273). Selectively transports sterols between membranes in vitro. Involved in stress-dependent formation of sterol-enriched vacuolar membrane domains (PubMed:25987606).<ref>PMID:25987606</ref> <ref>PMID:26001273</ref> <ref>PMID:26119743</ref> |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| | == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
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| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Bpt4]] | + | [[Category: Escherichia virus T4]] |
| - | [[Category: Lysozyme]] | + | [[Category: Large Structures]] |
| - | [[Category: Im, Y J]] | + | [[Category: Saccharomyces cerevisiae S288C]] |
| - | [[Category: Tong, J]] | + | [[Category: Im YJ]] |
| - | [[Category: Ligand binding domain]] | + | [[Category: Tong J]] |
| - | [[Category: Lipid transport]]
| + | |
| - | [[Category: Ltc1]]
| + | |
| - | [[Category: Sterol]]
| + | |
| - | [[Category: Transport protein]]
| + | |
| Structural highlights
Function
ENLYS_BPT4 Endolysin with lysozyme activity that degrades host peptidoglycans and participates with the holin and spanin proteins in the sequential events which lead to the programmed host cell lysis releasing the mature viral particles. Once the holin has permeabilized the host cell membrane, the endolysin can reach the periplasm and break down the peptidoglycan layer.[1] LAM6_YEAST Involved in regulation of various organellar membrane contact sites (PubMed:26119743). May be involved in sterol transfer between intracellular membranes (PubMed:26001273). Selectively transports sterols between membranes in vitro. Involved in stress-dependent formation of sterol-enriched vacuolar membrane domains (PubMed:25987606).[2] [3] [4]
Publication Abstract from PubMed
Membrane contact sites (MCSs) in eukaryotic cells are hotspots for lipid exchange, which is essential for many biological functions, including regulation of membrane properties and protein trafficking. Lipid transfer proteins anchored at membrane contact sites (LAMs) contain sterol-specific lipid transfer domains [StARkin domain (SD)] and multiple targeting modules to specific membrane organelles. Elucidating the structural mechanisms of targeting and ligand recognition by LAMs is important for understanding the interorganelle communication and exchange at MCSs. Here, we determined the crystal structures of the yeast Lam6 pleckstrin homology (PH)-like domain and the SDs of Lam2 and Lam4 in the apo form and in complex with ergosterol. The Lam6 PH-like domain displays a unique PH domain fold with a conserved N-terminal alpha-helix. The Lam6 PH-like domain lacks the basic surface for phosphoinositide binding, but contains hydrophobic patches on its surface, which are critical for targeting to endoplasmic reticulum (ER)-mitochondrial contacts. Structures of the LAM SDs display a helix-grip fold with a hydrophobic cavity and a flexible Omega1-loop as a lid. Ergosterol is bound to the pocket in a head-down orientation, with its hydrophobic acyl group located in the tunnel entrance. The Omega1-loop in an open conformation is essential for ergosterol binding by direct hydrophobic interaction. Structural comparison suggested that the sterol binding mode of the Lam2 SD2 is likely conserved among the sterol transfer proteins of the StARkin superfamily. Structural models of full-length Lam2 correlated with the sterol transport function at the membrane contact sites.
Structural basis of sterol recognition and nonvesicular transport by lipid transfer proteins anchored at membrane contact sites.,Tong J, Manik MK, Im YJ Proc Natl Acad Sci U S A. 2018 Jan 16. pii: 1719709115. doi:, 10.1073/pnas.1719709115. PMID:29339490[5]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Moussa SH, Kuznetsov V, Tran TA, Sacchettini JC, Young R. Protein determinants of phage T4 lysis inhibition. Protein Sci. 2012 Apr;21(4):571-82. doi: 10.1002/pro.2042. Epub 2012 Mar 2. PMID:22389108 doi:http://dx.doi.org/10.1002/pro.2042
- ↑ Murley A, Sarsam RD, Toulmay A, Yamada J, Prinz WA, Nunnari J. Ltc1 is an ER-localized sterol transporter and a component of ER-mitochondria and ER-vacuole contacts. J Cell Biol. 2015 May 25;209(4):539-48. PMID:25987606 doi:10.1083/jcb.201502033
- ↑ Gatta AT, Wong LH, Sere YY, Calderon-Norena DM, Cockcroft S, Menon AK, Levine TP. A new family of StART domain proteins at membrane contact sites has a role in ER-PM sterol transport. Elife. 2015 May 22;4. doi: 10.7554/eLife.07253. PMID:26001273 doi:http://dx.doi.org/10.7554/eLife.07253
- ↑ Elbaz-Alon Y, Eisenberg-Bord M, Shinder V, Stiller SB, Shimoni E, Wiedemann N, Geiger T, Schuldiner M. Lam6 Regulates the Extent of Contacts between Organelles. Cell Rep. 2015 Jul 7;12(1):7-14. PMID:26119743 doi:10.1016/j.celrep.2015.06.022
- ↑ Tong J, Manik MK, Im YJ. Structural basis of sterol recognition and nonvesicular transport by lipid transfer proteins anchored at membrane contact sites. Proc Natl Acad Sci U S A. 2018 Jan 16. pii: 1719709115. doi:, 10.1073/pnas.1719709115. PMID:29339490 doi:http://dx.doi.org/10.1073/pnas.1719709115
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