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| | ==Crystal structure of SSB complexed with myc== | | ==Crystal structure of SSB complexed with myc== |
| - | <StructureSection load='5yun' size='340' side='right' caption='[[5yun]], [[Resolution|resolution]] 2.67Å' scene=''> | + | <StructureSection load='5yun' size='340' side='right'caption='[[5yun]], [[Resolution|resolution]] 2.67Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[5yun]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Pseae Pseae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5YUN OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5YUN FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[5yun]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Pseudomonas_aeruginosa_PAO1 Pseudomonas aeruginosa PAO1]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5YUN OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5YUN FirstGlance]. <br> |
| - | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=MYC:3,5,7-TRIHYDROXY-2-(3,4,5-TRIHYDROXYPHENYL)-4H-CHROMEN-4-ONE'>MYC</scene></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.67Å</td></tr> |
| - | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">ssb, PA4232 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=208964 PSEAE])</td></tr> | + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MYC:3,5,7-TRIHYDROXY-2-(3,4,5-TRIHYDROXYPHENYL)-4H-CHROMEN-4-ONE'>MYC</scene></td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5yun FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5yun OCA], [http://pdbe.org/5yun PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5yun RCSB], [http://www.ebi.ac.uk/pdbsum/5yun PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5yun ProSAT]</span></td></tr> | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5yun FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5yun OCA], [https://pdbe.org/5yun PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5yun RCSB], [https://www.ebi.ac.uk/pdbsum/5yun PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5yun ProSAT]</span></td></tr> |
| | </table> | | </table> |
| | == Function == | | == Function == |
| - | [[http://www.uniprot.org/uniprot/SSB_PSEAE SSB_PSEAE]] Plays an important role in DNA replication, recombination and repair. Binds to ssDNA and to an array of partner proteins to recruit them to their sites of action during DNA metabolism.[HAMAP-Rule:MF_00984] | + | [https://www.uniprot.org/uniprot/SSB_PSEAE SSB_PSEAE] Plays an important role in DNA replication, recombination and repair. Binds to ssDNA and to an array of partner proteins to recruit them to their sites of action during DNA metabolism.[HAMAP-Rule:MF_00984] |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| | == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
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| | </div> | | </div> |
| | <div class="pdbe-citations 5yun" style="background-color:#fffaf0;"></div> | | <div class="pdbe-citations 5yun" style="background-color:#fffaf0;"></div> |
| | + | |
| | + | ==See Also== |
| | + | *[[Single-stranded DNA-binding protein 3D structures|Single-stranded DNA-binding protein 3D structures]] |
| | == References == | | == References == |
| | <references/> | | <references/> |
| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Pseae]] | + | [[Category: Large Structures]] |
| - | [[Category: Huang, C Y]] | + | [[Category: Pseudomonas aeruginosa PAO1]] |
| - | [[Category: Huang, Y H]] | + | [[Category: Huang CY]] |
| - | [[Category: Dna binding protein]] | + | [[Category: Huang YH]] |
| - | [[Category: Single-strand dna binding protein]]
| + | |
| Structural highlights
Function
SSB_PSEAE Plays an important role in DNA replication, recombination and repair. Binds to ssDNA and to an array of partner proteins to recruit them to their sites of action during DNA metabolism.[HAMAP-Rule:MF_00984]
Publication Abstract from PubMed
Single-stranded DNA-binding protein (SSB) is essential for all DNA-dependent cellular processes. SSB inhibitors have been recently suggested as broad-spectrum antibacterial agents in antibiotic development. In this paper, we report the first inhibitor-complexed crystal structure of SSB from Pseudomonas aeruginosa PAO1 (PaSSB) at 2.68A resolution (PDB entry 5YUN). The inhibitor, myricetin, is a flavonol that possesses many pharmacological activities, such as anticancer, anti-inflammatory, and antibacterial properties, and is beneficial for humans. Four monomers of PaSSB and two of myricetins were found per asymmetric unit. Various interactions between myricetin and PaSSB were examined. Among these, four residues in PaSSB, Lys7, Arg62, Glu80, and Gly107 were found crucial for forming hydrogen bond to myricetin. These two myricetins occupy the grooves for ssDNA-binding of SSB that may prevent ssDNA-wrapping and ssDNA-binding stably from SSB. In addition to explaining how SSB can be inhibited, the myricetin-SSB interaction modes in this paper may also provide insights into how myricetin can bind and inhibit proteins on cancer-signaling pathways.
Crystal structure of SSB complexed with inhibitor myricetin.,Huang CY Biochem Biophys Res Commun. 2018 Oct 12;504(4):704-708. doi:, 10.1016/j.bbrc.2018.08.188. Epub 2018 Sep 11. PMID:30213633[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
See Also
References
- ↑ Huang CY. Crystal structure of SSB complexed with inhibitor myricetin. Biochem Biophys Res Commun. 2018 Oct 12;504(4):704-708. doi:, 10.1016/j.bbrc.2018.08.188. Epub 2018 Sep 11. PMID:30213633 doi:http://dx.doi.org/10.1016/j.bbrc.2018.08.188
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