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| | ==Crystal structure of C-terminal fragment of SpaD from Lactobacillus rhamnosus GG generated by limited proteolysis== | | ==Crystal structure of C-terminal fragment of SpaD from Lactobacillus rhamnosus GG generated by limited proteolysis== |
| - | <StructureSection load='5yxg' size='340' side='right' caption='[[5yxg]], [[Resolution|resolution]] 1.48Å' scene=''> | + | <StructureSection load='5yxg' size='340' side='right'caption='[[5yxg]], [[Resolution|resolution]] 1.48Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[5yxg]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Lacrg Lacrg]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5YXG OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5YXG FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[5yxg]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Lacticaseibacillus_rhamnosus_GG Lacticaseibacillus rhamnosus GG]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5YXG OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5YXG FirstGlance]. <br> |
| - | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.48Å</td></tr> |
| - | <tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=SME:METHIONINE+SULFOXIDE'>SME</scene></td></tr> | + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=SME:METHIONINE+SULFOXIDE'>SME</scene></td></tr> |
| - | <tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[5yu5|5yu5]]</td></tr>
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5yxg FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5yxg OCA], [https://pdbe.org/5yxg PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5yxg RCSB], [https://www.ebi.ac.uk/pdbsum/5yxg PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5yxg ProSAT]</span></td></tr> |
| - | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">CCE29_10230, PY66_09460 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=568703 LACRG])</td></tr>
| + | |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5yxg FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5yxg OCA], [http://pdbe.org/5yxg PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5yxg RCSB], [http://www.ebi.ac.uk/pdbsum/5yxg PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5yxg ProSAT]</span></td></tr> | + | |
| | </table> | | </table> |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
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| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Lacrg]] | + | [[Category: Lacticaseibacillus rhamnosus GG]] |
| - | [[Category: Chaurasia, P]] | + | [[Category: Large Structures]] |
| - | [[Category: Krishnan, V]] | + | [[Category: Chaurasia P]] |
| - | [[Category: Ossowski, I von]] | + | [[Category: Krishnan V]] |
| - | [[Category: Palva, A]] | + | [[Category: Palva A]] |
| - | [[Category: Pratap, S]] | + | [[Category: Pratap S]] |
| - | [[Category: Adhesin]] | + | [[Category: Von Ossowski I]] |
| - | [[Category: Cell adhesion]]
| + | |
| - | [[Category: Isopeptide]]
| + | |
| - | [[Category: Pilin]]
| + | |
| - | [[Category: Probiotic]]
| + | |
| - | [[Category: Spad]]
| + | |
| - | [[Category: Spafed pili]]
| + | |
| Structural highlights
Publication Abstract from PubMed
Effective colonization of host cells by some Gram-positive bacteria often involves using lengthy, adhesive macromolecular structures called sortase-dependent pili. Among commensals, the gut-adapted Lactobacillus rhamnosus GG strain encodes the operons for two varieties of these pili (SpaCBA and SpaFED), with each structure consisting of backbone, tip, and basal pilin subunits. Although the tertiary structure was recently solved for the backbone subunit (SpaA) of the SpaCBA pilus, no structural information exists for its counterpart in the SpaFED pilus. Here, we report several crystal structures for the SpaD backbone pilin, two of which capture the N-terminal domain in either the closed (linear) or open (bent) conformation. To our knowledge, this is the first observation of the bent conformation in Gram-positive pilin structures. Based on this bent conformation, we suggest a three-stage model, which we call the expose-ligate-seal mechanism, for the docking and assembly of backbone pilins into the sortase-dependent pilus.
Bent conformation of a backbone pilin N-terminal domain supports a three-stage pilus assembly mechanism.,Chaurasia P, Pratap S, Palva A, von Ossowski I, Krishnan V Commun Biol. 2018 Jul 17;1:94. doi: 10.1038/s42003-018-0100-0. eCollection 2018. PMID:30271975[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Chaurasia P, Pratap S, Palva A, von Ossowski I, Krishnan V. Bent conformation of a backbone pilin N-terminal domain supports a three-stage pilus assembly mechanism. Commun Biol. 2018 Jul 17;1:94. doi: 10.1038/s42003-018-0100-0. eCollection 2018. PMID:30271975 doi:http://dx.doi.org/10.1038/s42003-018-0100-0
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