1pjm

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(New page: 200px<br /> <applet load="1pjm" size="450" color="white" frame="true" align="right" spinBox="true" caption="1pjm, resolution 2.50&Aring;" /> '''Mouse Importin alph...)
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Revision as of 16:37, 12 November 2007

Image:1pjm.gif

1pjm, resolution 2.50Å

Drag the structure with the mouse to rotate

Mouse Importin alpha-bipartite NLS from human retinoblastoma protein Complex

Contents

Overview

Importin-alpha is the nuclear import receptor that recognizes cargo, proteins carrying conventional basic monopartite and bipartite nuclear, localization sequences (NLSs) and facilitates their transport into the, nucleus. Bipartite NLSs contain two clusters of basic residues, connected, by linkers of variable lengths. To determine the structural basis of the, recognition of diverse bipartite NLSs by mammalian importin-alpha, we, co-crystallized a non-autoinhibited mouse receptor protein with peptides, corresponding to the NLSs from human retinoblastoma protein and Xenopus, laevis phosphoprotein N1N2, containing diverse sequences and lengths of, the linker. We show that the basic clusters interact analogously in both, NLSs, but the linker sequences adopt different conformations, whereas both, make specific contacts with the receptor. The available data allow us to, draw general conclusions about the specificity of NLS binding by, importin-alpha and facilitate an improved definition of the consensus, sequence of a conventional basic/bipartite NLS (KRX10-12KRRK) that can be, used to identify novel nuclear proteins.

Disease

Known diseases associated with this structure: Bladder cancer OMIM:[180200], Osteosarcoma OMIM:[180200], Pinealoma with bilateral retinoblastoma OMIM:[180200], Retinoblastoma OMIM:[180200]

About this Structure

1PJM is a Protein complex structure of sequences from Mus musculus. Full crystallographic information is available from OCA.

Reference

Structural basis for the specificity of bipartite nuclear localization sequence binding by importin-alpha., Fontes MR, Teh T, Jans D, Brinkworth RI, Kobe B, J Biol Chem. 2003 Jul 25;278(30):27981-7. Epub 2003 Apr 14. PMID:12695505

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