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| | ==Crystal structure of human LRRTM2== | | ==Crystal structure of human LRRTM2== |
| - | <StructureSection load='5z8x' size='340' side='right' caption='[[5z8x]], [[Resolution|resolution]] 3.15Å' scene=''> | + | <StructureSection load='5z8x' size='340' side='right'caption='[[5z8x]], [[Resolution|resolution]] 3.15Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[5z8x]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5Z8X OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5Z8X FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[5z8x]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5Z8X OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5Z8X FirstGlance]. <br> |
| - | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 3.15Å</td></tr> |
| - | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">LRRTM2, KIAA0416, LRRN2 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])</td></tr> | + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene></td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5z8x FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5z8x OCA], [http://pdbe.org/5z8x PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5z8x RCSB], [http://www.ebi.ac.uk/pdbsum/5z8x PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5z8x ProSAT]</span></td></tr> | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5z8x FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5z8x OCA], [https://pdbe.org/5z8x PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5z8x RCSB], [https://www.ebi.ac.uk/pdbsum/5z8x PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5z8x ProSAT]</span></td></tr> |
| | </table> | | </table> |
| | == Function == | | == Function == |
| - | [[http://www.uniprot.org/uniprot/LRRT2_HUMAN LRRT2_HUMAN]] Involved in the development and maintenance of excitatory synapse in the vertebrate nervous system. Regulates surface expression of AMPA receptors and instructs the development of functional glutamate release sites. Acts as a ligand for the presynaptic receptors NRXN1-A and NRXN1-B (By similarity). | + | [https://www.uniprot.org/uniprot/LRRT2_HUMAN LRRT2_HUMAN] Involved in the development and maintenance of excitatory synapse in the vertebrate nervous system. Regulates surface expression of AMPA receptors and instructs the development of functional glutamate release sites. Acts as a ligand for the presynaptic receptors NRXN1-A and NRXN1-B (By similarity). |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| | == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
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| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Human]] | + | [[Category: Homo sapiens]] |
| - | [[Category: Fukai, S]] | + | [[Category: Large Structures]] |
| - | [[Category: Yamagata, A]] | + | [[Category: Fukai S]] |
| - | [[Category: Cell adhesion]] | + | [[Category: Yamagata A]] |
| - | [[Category: Leucine-rich repeat]]
| + | |
| - | [[Category: Synapse]]
| + | |
| - | [[Category: Synaptic organizer]]
| + | |
| Structural highlights
Function
LRRT2_HUMAN Involved in the development and maintenance of excitatory synapse in the vertebrate nervous system. Regulates surface expression of AMPA receptors and instructs the development of functional glutamate release sites. Acts as a ligand for the presynaptic receptors NRXN1-A and NRXN1-B (By similarity).
Publication Abstract from PubMed
Leucine-rich repeat transmembrane neuronal proteins (LRRTMs) function as postsynaptic organizers that induce excitatory synapses. Neurexins (Nrxns) and heparan sulfate proteoglycans have been identified as presynaptic ligands for LRRTMs. Specifically, LRRTM1 and LRRTM2 bind to the Nrxn splice variant lacking an insert at the splice site 4 (S4). Here, we report the crystal structure of the Nrxn1beta-LRRTM2 complex at 3.4 A resolution. The Nrxn1beta-LRRTM2 interface involves Ca(2+)-mediated interactions and overlaps with the Nrxn-neuroligin interface. Together with structure-based mutational analyses at the molecular and cellular levels, the present structural analysis unveils the mechanism of selective binding between Nrxn and LRRTM1/2 and its modulation by the S4 insertion of Nrxn.
Structural insights into modulation and selectivity of transsynaptic neurexin-LRRTM interaction.,Yamagata A, Goto-Ito S, Sato Y, Shiroshima T, Maeda A, Watanabe M, Saitoh T, Maenaka K, Terada T, Yoshida T, Uemura T, Fukai S Nat Commun. 2018 Sep 27;9(1):3964. doi: 10.1038/s41467-018-06333-8. PMID:30262834[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Yamagata A, Goto-Ito S, Sato Y, Shiroshima T, Maeda A, Watanabe M, Saitoh T, Maenaka K, Terada T, Yoshida T, Uemura T, Fukai S. Structural insights into modulation and selectivity of transsynaptic neurexin-LRRTM interaction. Nat Commun. 2018 Sep 27;9(1):3964. doi: 10.1038/s41467-018-06333-8. PMID:30262834 doi:http://dx.doi.org/10.1038/s41467-018-06333-8
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