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| | ==Crystal structure of Sialic acid Binding protein from Haemophilus ducreyi== | | ==Crystal structure of Sialic acid Binding protein from Haemophilus ducreyi== |
| - | <StructureSection load='5za4' size='340' side='right' caption='[[5za4]], [[Resolution|resolution]] 2.19Å' scene=''> | + | <StructureSection load='5za4' size='340' side='right'caption='[[5za4]], [[Resolution|resolution]] 2.19Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[5za4]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Haedu Haedu]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5ZA4 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5ZA4 FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[5za4]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Haemophilus_ducreyi_35000HP Haemophilus ducreyi 35000HP]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5ZA4 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5ZA4 FirstGlance]. <br> |
| - | </td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">HD_1669 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=233412 HAEDU])</td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.193Å</td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5za4 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5za4 OCA], [http://pdbe.org/5za4 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5za4 RCSB], [http://www.ebi.ac.uk/pdbsum/5za4 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5za4 ProSAT]</span></td></tr> | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5za4 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5za4 OCA], [https://pdbe.org/5za4 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5za4 RCSB], [https://www.ebi.ac.uk/pdbsum/5za4 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5za4 ProSAT]</span></td></tr> |
| | </table> | | </table> |
| | + | == Function == |
| | + | [https://www.uniprot.org/uniprot/Q7VL18_HAEDU Q7VL18_HAEDU] |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| | == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
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| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Haedu]] | + | [[Category: Large Structures]] |
| - | [[Category: Setty, T G]] | + | [[Category: Setty TG]] |
| - | [[Category: Subramanian, R]] | + | [[Category: Subramanian R]] |
| - | [[Category: Adhesion]]
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| - | [[Category: Cell signaling]]
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| - | [[Category: Molecular mimicry]]
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| - | [[Category: Nutrition]]
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| - | [[Category: Sialic acid neu5gc]]
| + | |
| - | [[Category: Sugar binding protein]]
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| - | [[Category: Three domain protein]]
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| Structural highlights
Function
Q7VL18_HAEDU
Publication Abstract from PubMed
The primary role of bacterial periplasmic binding proteins is sequestration of essential metabolites present at a low concentration in the periplasm and making them available for active transporters that transfer these ligands into the bacterial cell. The periplasmic binding proteins(SiaP) from the tripartite ATP-independent periplasmic (TRAP) transport system that transports mammalian host-derived sialic acids, have been well studied from different pathogenic bacteria, including Haemophilus influenzae, Fusobacterium nucleatum, Pasteurella multocida, and Vibrio cholerae SiaPs bind the sialic acid N-acetylneuraminic acid (Neu5Ac) with nanomolar affinity by forming electrostatic and hydrogen bonding interactions. Here, we report the crystal structure of a periplasmic binding protein (SatA) of the ATP-binding cassette (ABC) transport system from the pathogenic bacterium Haemophilus ducreyi The structure of Hd-SatA in the native form and sialic acid-bound forms (with Neu5Ac and N-glycolylneuraminic acid [Neu5Gc]), determined to 2.2, 1.5 and 2.5 A resolutions respectively, revealed a ligand-binding site that is very different from those of the SiaPs of the TRAP transport system. A structural comparison along with thermodynamic studies suggested that similar affinities are achieved in the two classes of proteins through distinct mechanisms, one enthalpically driven and the other entropically driven. In summary, our structural and thermodynamic characterization of Hd-SatA reveal that it binds sialic acids with nanomolar affinity and this binding is an entropically driven process. This information is important for future structure-based drug design against this pathogen and related bacteria.
Molecular characterization of the interaction of sialic acid with the periplasmic binding protein from Haemophilus ducreyi.,Setty TG, Mowers JC, Hobbs AG, Maiya SP, Syed S, Munson RS Jr, Apicella MA, Subramanian R J Biol Chem. 2018 Oct 12. pii: RA118.005151. doi: 10.1074/jbc.RA118.005151. PMID:30315109[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Setty TG, Mowers JC, Hobbs AG, Maiya SP, Syed S, Munson RS Jr, Apicella MA, Subramanian R. Molecular characterization of the interaction of sialic acid with the periplasmic binding protein from Haemophilus ducreyi. J Biol Chem. 2018 Oct 12. pii: RA118.005151. doi: 10.1074/jbc.RA118.005151. PMID:30315109 doi:http://dx.doi.org/10.1074/jbc.RA118.005151
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