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| | ==Crystal structure of Rtt109 from Aspergillus fumigatus== | | ==Crystal structure of Rtt109 from Aspergillus fumigatus== |
| - | <StructureSection load='5zb9' size='340' side='right' caption='[[5zb9]], [[Resolution|resolution]] 2.50Å' scene=''> | + | <StructureSection load='5zb9' size='340' side='right'caption='[[5zb9]], [[Resolution|resolution]] 2.50Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[5zb9]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Aspfu Aspfu]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5ZB9 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5ZB9 FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[5zb9]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Aspergillus_fumigatus_Af293 Aspergillus fumigatus Af293]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5ZB9 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5ZB9 FirstGlance]. <br> |
| - | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.502Å</td></tr> |
| - | <tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=ALY:N(6)-ACETYLLYSINE'>ALY</scene></td></tr> | + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ALY:N(6)-ACETYLLYSINE'>ALY</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene></td></tr> |
| - | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">AFUA_5G09540 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=330879 ASPFU])</td></tr>
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5zb9 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5zb9 OCA], [https://pdbe.org/5zb9 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5zb9 RCSB], [https://www.ebi.ac.uk/pdbsum/5zb9 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5zb9 ProSAT]</span></td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5zb9 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5zb9 OCA], [http://pdbe.org/5zb9 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5zb9 RCSB], [http://www.ebi.ac.uk/pdbsum/5zb9 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5zb9 ProSAT]</span></td></tr> | + | |
| | </table> | | </table> |
| | + | == Function == |
| | + | [https://www.uniprot.org/uniprot/Q4WUS9_ASPFU Q4WUS9_ASPFU] |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| | == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
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| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Aspfu]] | + | [[Category: Aspergillus fumigatus Af293]] |
| - | [[Category: Serra-Cardona, A]] | + | [[Category: Large Structures]] |
| - | [[Category: Wang, M]] | + | [[Category: Serra-Cardona A]] |
| - | [[Category: Xu, R M]] | + | [[Category: Wang M]] |
| - | [[Category: Yang, N]] | + | [[Category: Xu RM]] |
| - | [[Category: Zhang, L]] | + | [[Category: Yang N]] |
| - | [[Category: Zhang, Z]] | + | [[Category: Zhang L]] |
| - | [[Category: Zhou, H]] | + | [[Category: Zhang Z]] |
| - | [[Category: Acetylation]]
| + | [[Category: Zhou H]] |
| - | [[Category: Dna damage]]
| + | |
| - | [[Category: Dna replication]]
| + | |
| - | [[Category: Histone]]
| + | |
| - | [[Category: Nucleosome assembly]]
| + | |
| - | [[Category: Transferase]]
| + | |
| Structural highlights
Function
Q4WUS9_ASPFU
Publication Abstract from PubMed
Rtt109 is a unique histone acetyltransferase acetylating histone H3 lysine 56 (H3K56), a modification critical for DNA replication-coupled nucleosome assembly and genome stability. In cells, histone chaperone Asf1 is essential for H3K56 acetylation, yet the mechanisms for H3K56 specificity and Asf1 requirement remain unknown. We have determined the crystal structure of the Rtt109-Asf1-H3-H4 complex and found that unwinding of histone H3 alphaN, where K56 is normally located, and stabilization of the very C-terminal beta strand of histone H4 by Asf1 are prerequisites for H3K56 acetylation. Unexpectedly, an interaction between Rtt109 and the central helix of histone H3 is also required. The observed multiprotein, multisite substrate recognition mechanism among histone modification enzymes provides mechanistic understandings of Rtt109 and Asf1 in H3K56 acetylation, as well as valuable insights into substrate recognition by histone modification enzymes in general.
Multisite Substrate Recognition in Asf1-Dependent Acetylation of Histone H3 K56 by Rtt109.,Zhang L, Serra-Cardona A, Zhou H, Wang M, Yang N, Zhang Z, Xu RM Cell. 2018 Aug 9;174(4):818-830.e11. doi: 10.1016/j.cell.2018.07.005. Epub 2018, Jul 26. PMID:30057113[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Zhang L, Serra-Cardona A, Zhou H, Wang M, Yang N, Zhang Z, Xu RM. Multisite Substrate Recognition in Asf1-Dependent Acetylation of Histone H3 K56 by Rtt109. Cell. 2018 Aug 9;174(4):818-830.e11. doi: 10.1016/j.cell.2018.07.005. Epub 2018, Jul 26. PMID:30057113 doi:http://dx.doi.org/10.1016/j.cell.2018.07.005
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