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| | ==Crystal structure of MBP-fused BIL1/BZR1 in complex with double-stranded DNA== | | ==Crystal structure of MBP-fused BIL1/BZR1 in complex with double-stranded DNA== |
| - | <StructureSection load='5zd4' size='340' side='right' caption='[[5zd4]], [[Resolution|resolution]] 2.17Å' scene=''> | + | <StructureSection load='5zd4' size='340' side='right'caption='[[5zd4]], [[Resolution|resolution]] 2.17Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[5zd4]] is a 8 chain structure with sequence from [http://en.wikipedia.org/wiki/Arath Arath]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5ZD4 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5ZD4 FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[5zd4]] is a 8 chain structure with sequence from [https://en.wikipedia.org/wiki/Arabidopsis_thaliana Arabidopsis thaliana], [https://en.wikipedia.org/wiki/Escherichia_coli_O157:H7 Escherichia coli O157:H7] and [https://en.wikipedia.org/wiki/Synthetic_construct Synthetic construct]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5ZD4 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5ZD4 FirstGlance]. <br> |
| - | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=MAL:MALTOSE'>MAL</scene></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.17Å</td></tr> |
| - | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">BZR1, BIS2, At1g75080, F9E10_7 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=3702 ARATH])</td></tr> | + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=GLC:ALPHA-D-GLUCOSE'>GLC</scene>, <scene name='pdbligand=PRD_900001:alpha-maltose'>PRD_900001</scene></td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5zd4 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5zd4 OCA], [http://pdbe.org/5zd4 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5zd4 RCSB], [http://www.ebi.ac.uk/pdbsum/5zd4 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5zd4 ProSAT]</span></td></tr> | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5zd4 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5zd4 OCA], [https://pdbe.org/5zd4 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5zd4 RCSB], [https://www.ebi.ac.uk/pdbsum/5zd4 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5zd4 ProSAT]</span></td></tr> |
| | </table> | | </table> |
| | == Function == | | == Function == |
| - | [[http://www.uniprot.org/uniprot/MALE_ECO57 MALE_ECO57]] Involved in the high-affinity maltose membrane transport system MalEFGK. Initial receptor for the active transport of and chemotaxis toward maltooligosaccharides (By similarity). | + | [https://www.uniprot.org/uniprot/MALE_ECO57 MALE_ECO57] Involved in the high-affinity maltose membrane transport system MalEFGK. Initial receptor for the active transport of and chemotaxis toward maltooligosaccharides (By similarity).[https://www.uniprot.org/uniprot/BZR1_ARATH BZR1_ARATH] Transcriptional repressor that binds to the brassinosteroid (BR) response element (BRRE) 5'-CGTG(T/C)G-3' in gene promoter. Regulates positively the brassinosteroid-signaling pathway (PubMed:33324437). Mediates downstream growth responses and negative feedback regulation of brassinosteroid biosynthesis. Promotes growth. Modulates ovule initiation and development by monitoring the expression of genes related to ovule development (e.g. HLL, ANT, and AP2). Regulates negatively the abscisic acid (ABA) signaling pathway during the post-germination stage (PubMed:33324437).<ref>PMID:15681342</ref> <ref>PMID:22914576</ref> <ref>PMID:33324437</ref> |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| | == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
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| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Arath]] | + | [[Category: Arabidopsis thaliana]] |
| - | [[Category: Hirabayashi, K]] | + | [[Category: Escherichia coli O157:H7]] |
| - | [[Category: Miyakawa, T]]
| + | [[Category: Large Structures]] |
| - | [[Category: Nakamura, A]] | + | [[Category: Synthetic construct]] |
| - | [[Category: Nosaki, S]] | + | [[Category: Hirabayashi K]] |
| - | [[Category: Tanokura, M]] | + | [[Category: Miyakawa T]] |
| - | [[Category: Xu, Y]] | + | [[Category: Nakamura A]] |
| - | [[Category: Brassinosteroid]] | + | [[Category: Nosaki S]] |
| - | [[Category: Plant]] | + | [[Category: Tanokura M]] |
| - | [[Category: Transcription]] | + | [[Category: Xu Y]] |
| - | [[Category: Transcription factor]] | + | |
| Structural highlights
Function
MALE_ECO57 Involved in the high-affinity maltose membrane transport system MalEFGK. Initial receptor for the active transport of and chemotaxis toward maltooligosaccharides (By similarity).BZR1_ARATH Transcriptional repressor that binds to the brassinosteroid (BR) response element (BRRE) 5'-CGTG(T/C)G-3' in gene promoter. Regulates positively the brassinosteroid-signaling pathway (PubMed:33324437). Mediates downstream growth responses and negative feedback regulation of brassinosteroid biosynthesis. Promotes growth. Modulates ovule initiation and development by monitoring the expression of genes related to ovule development (e.g. HLL, ANT, and AP2). Regulates negatively the abscisic acid (ABA) signaling pathway during the post-germination stage (PubMed:33324437).[1] [2] [3]
Publication Abstract from PubMed
BRZ-INSENSITIVE-LONG HYPOCOTYL 1 (BIL1)/BRASSINAZOLE-RESISTANT 1 (BZR1) is a master transcription factor of brassinosteroid (BR) signalling. The varieties of nucleobase recognition of the NN-BRRE-core motif (NNCGTG), one of variant G-box motifs, distinguish BIL1/BZR1 from basic helix-loop-helix transcription factors, underlying the specific regulation of BR-responsive genes. Here, we show the non-canonical bHLH dimer formation of BIL1/BZR1 to optimize the interaction network with DNA and the orientation of a key residue for NN-BRRE-core motif recognition.
Structural basis for brassinosteroid response by BIL1/BZR1.,Nosaki S, Miyakawa T, Xu Y, Nakamura A, Hirabayashi K, Asami T, Nakano T, Tanokura M Nat Plants. 2018 Oct;4(10):771-776. doi: 10.1038/s41477-018-0255-1. Epub 2018 Oct, 1. PMID:30287951[4]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ He JX, Gendron JM, Sun Y, Gampala SS, Gendron N, Sun CQ, Wang ZY. BZR1 is a transcriptional repressor with dual roles in brassinosteroid homeostasis and growth responses. Science. 2005 Mar 11;307(5715):1634-8. doi: 10.1126/science.1107580. Epub 2005 , Jan 27. PMID:15681342 doi:http://dx.doi.org/10.1126/science.1107580
- ↑ Huang HY, Jiang WB, Hu YW, Wu P, Zhu JY, Liang WQ, Wang ZY, Lin WH. BR signal influences Arabidopsis ovule and seed number through regulating related genes expression by BZR1. Mol Plant. 2013 Mar;6(2):456-69. doi: 10.1093/mp/sss070. Epub 2012 Aug 22. PMID:22914576 doi:http://dx.doi.org/10.1093/mp/sss070
- ↑ Wu J, Yan M, Zhang D, Zhou D, Yamaguchi N, Ito T. Histone Demethylases Coordinate the Antagonistic Interaction Between Abscisic Acid and Brassinosteroid Signaling in Arabidopsis. Front Plant Sci. 2020 Nov 25;11:596835. doi: 10.3389/fpls.2020.596835. , eCollection 2020. PMID:33324437 doi:http://dx.doi.org/10.3389/fpls.2020.596835
- ↑ Nosaki S, Miyakawa T, Xu Y, Nakamura A, Hirabayashi K, Asami T, Nakano T, Tanokura M. Structural basis for brassinosteroid response by BIL1/BZR1. Nat Plants. 2018 Oct;4(10):771-776. doi: 10.1038/s41477-018-0255-1. Epub 2018 Oct, 1. PMID:30287951 doi:http://dx.doi.org/10.1038/s41477-018-0255-1
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