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| | ==Crystal structure of mouse nectin-like molecule 4 (mNecl-4) full ectodomain in complex with mouse nectin-like molecule 1 (mNecl-1) Ig1 domain, 3.3A== | | ==Crystal structure of mouse nectin-like molecule 4 (mNecl-4) full ectodomain in complex with mouse nectin-like molecule 1 (mNecl-1) Ig1 domain, 3.3A== |
| - | <StructureSection load='5zo2' size='340' side='right' caption='[[5zo2]], [[Resolution|resolution]] 3.29Å' scene=''> | + | <StructureSection load='5zo2' size='340' side='right'caption='[[5zo2]], [[Resolution|resolution]] 3.29Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[5zo2]] is a 3 chain structure with sequence from [http://en.wikipedia.org/wiki/Lk3_transgenic_mice Lk3 transgenic mice]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5ZO2 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5ZO2 FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[5zo2]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5ZO2 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5ZO2 FirstGlance]. <br> |
| - | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=FUC:ALPHA-L-FUCOSE'>FUC</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 3.29Å</td></tr> |
| - | <tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[1z9m|1z9m]], [[5zo1|5zo1]]</td></tr> | + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=FUC:ALPHA-L-FUCOSE'>FUC</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene></td></tr> |
| - | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">Cadm4, Igsf4c, Necl4, Tsll2 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10090 LK3 transgenic mice]), Cadm3, Igsf4b, Necl1, Syncam3, Tsll1 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10090 LK3 transgenic mice])</td></tr>
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5zo2 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5zo2 OCA], [https://pdbe.org/5zo2 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5zo2 RCSB], [https://www.ebi.ac.uk/pdbsum/5zo2 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5zo2 ProSAT]</span></td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5zo2 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5zo2 OCA], [http://pdbe.org/5zo2 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5zo2 RCSB], [http://www.ebi.ac.uk/pdbsum/5zo2 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5zo2 ProSAT]</span></td></tr> | + | |
| | </table> | | </table> |
| | == Function == | | == Function == |
| - | [[http://www.uniprot.org/uniprot/CADM4_MOUSE CADM4_MOUSE]] Involved in the cell-cell adhesion. Has calcium- and magnesium-independent cell-cell adhesion activity. May have tumor-suppressor activity.<ref>PMID:14659875</ref> [[http://www.uniprot.org/uniprot/CADM3_MOUSE CADM3_MOUSE]] Involved in the cell-cell adhesion. Has both calcium-independent homophilic cell-cell adhesion activity and calcium-independent heterophilic cell-cell adhesion activity with IGSF4, NECTIN1 and NECTIN3. Interaction with EPB41L1 may regulate structure or function of cell-cell junctions.<ref>PMID:15741237</ref> <ref>PMID:15893517</ref> | + | [https://www.uniprot.org/uniprot/CADM4_MOUSE CADM4_MOUSE] Involved in the cell-cell adhesion. Has calcium- and magnesium-independent cell-cell adhesion activity. May have tumor-suppressor activity.<ref>PMID:14659875</ref> |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| | == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
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| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Lk3 transgenic mice]] | + | [[Category: Large Structures]] |
| - | [[Category: An, T]] | + | [[Category: Mus musculus]] |
| - | [[Category: Cui, S]] | + | [[Category: An T]] |
| - | [[Category: Fan, Z]] | + | [[Category: Cui S]] |
| - | [[Category: Hu, G]] | + | [[Category: Fan Z]] |
| - | [[Category: Ju, W]] | + | [[Category: Hu G]] |
| - | [[Category: Li, C]] | + | [[Category: Ju W]] |
| - | [[Category: Li, D]] | + | [[Category: Li C]] |
| - | [[Category: Li, J]] | + | [[Category: Li D]] |
| - | [[Category: Liu, X]] | + | [[Category: Li J]] |
| - | [[Category: Peng, X]] | + | [[Category: Liu X]] |
| - | [[Category: Qiang, B]] | + | [[Category: Peng X]] |
| - | [[Category: Qin, B]] | + | [[Category: Qiang B]] |
| - | [[Category: Shu, P]] | + | [[Category: Qin B]] |
| - | [[Category: Wang, M]] | + | [[Category: Shu P]] |
| - | [[Category: Wojdyla, J A]] | + | [[Category: Wang M]] |
| - | [[Category: Xiang, P]] | + | [[Category: Wojdyla JA]] |
| - | [[Category: Yin, B]] | + | [[Category: Xiang P]] |
| - | [[Category: Yuan, J]] | + | [[Category: Yin B]] |
| - | [[Category: Axon]]
| + | [[Category: Yuan J]] |
| - | [[Category: Cadm]]
| + | |
| - | [[Category: Cell adhesion]]
| + | |
| - | [[Category: Cell adhesion molecule]]
| + | |
| - | [[Category: Glycoprotein]]
| + | |
| - | [[Category: Heterogeneous dimer]]
| + | |
| - | [[Category: Ig domain]]
| + | |
| - | [[Category: Molecular replacement]]
| + | |
| - | [[Category: Myelogenesis]]
| + | |
| - | [[Category: Necl-1]]
| + | |
| - | [[Category: Necl-4]]
| + | |
| - | [[Category: Nectin-like]]
| + | |
| - | [[Category: Schwann cell]]
| + | |
| - | [[Category: Syncam]]
| + | |
| Structural highlights
Function
CADM4_MOUSE Involved in the cell-cell adhesion. Has calcium- and magnesium-independent cell-cell adhesion activity. May have tumor-suppressor activity.[1]
Publication Abstract from PubMed
Nectin-like (Necl) molecules are Ca(2+)-independent Ig-like transmembrane cell adhesion molecules that participate in junctions between different cell types. The specific cell-cell adhesions mediated by Necl proteins are important in neural development and have been implicated in neurodegenerative diseases. Here, we present the crystal structure of the mouse Necl-4 full ectodomain and the structure of the heterophilic Necl ectodomain complex formed by the mNecl-4 and mNecl-1 ectodomains. We demonstrate that, while the ectodomain of mNecl-4 is monomeric, it forms a stable heterodimer with Ig1 of mNecl-1, with an affinity significantly higher than that observed for self-dimerization of the mNecl-1 ectodomain. We validated our structural characterizations by performing a surface plasmon resonance assay and an Fc fusion protein binding assay in mouse primary dorsal root ganglia neurites and Schwann cells and identified a selection of residues important for heterophilic interactions. Finally, we proposed a model of Necl binding specificity that involves an induced-fit conformational change at the dimerization interface.
Structure of the heterophilic interaction between the nectin-like 4 and nectin-like 1 molecules.,Liu X, An T, Li D, Fan Z, Xiang P, Li C, Ju W, Li J, Hu G, Qin B, Yin B, Wojdyla JA, Wang M, Yuan J, Qiang B, Shu P, Cui S, Peng X Proc Natl Acad Sci U S A. 2019 Feb 5;116(6):2068-2077. doi:, 10.1073/pnas.1810969116. Epub 2019 Jan 23. PMID:30674679[2]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Fukami T, Satoh H, Williams YN, Masuda M, Fukuhara H, Maruyama T, Yageta M, Kuramochi M, Takamoto S, Murakami Y. Isolation of the mouse Tsll1 and Tsll2 genes, orthologues of the human TSLC1-like genes 1 and 2 (TSLL1 and TSLL2). Gene. 2003 Dec 24;323:11-8. PMID:14659875
- ↑ Liu X, An T, Li D, Fan Z, Xiang P, Li C, Ju W, Li J, Hu G, Qin B, Yin B, Wojdyla JA, Wang M, Yuan J, Qiang B, Shu P, Cui S, Peng X. Structure of the heterophilic interaction between the nectin-like 4 and nectin-like 1 molecules. Proc Natl Acad Sci U S A. 2019 Feb 5;116(6):2068-2077. doi:, 10.1073/pnas.1810969116. Epub 2019 Jan 23. PMID:30674679 doi:http://dx.doi.org/10.1073/pnas.1810969116
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