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| | ==Crystal Structure of Penicillin-Binding Protein D2 from Listeria monocytogenes in the Cefotaxime bound form== | | ==Crystal Structure of Penicillin-Binding Protein D2 from Listeria monocytogenes in the Cefotaxime bound form== |
| - | <StructureSection load='5zqd' size='340' side='right' caption='[[5zqd]], [[Resolution|resolution]] 1.89Å' scene=''> | + | <StructureSection load='5zqd' size='340' side='right'caption='[[5zqd]], [[Resolution|resolution]] 1.89Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[5zqd]] is a 8 chain structure with sequence from [http://en.wikipedia.org/wiki/Lismo Lismo]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5ZQD OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5ZQD FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[5zqd]] is a 8 chain structure with sequence from [https://en.wikipedia.org/wiki/Listeria_monocytogenes_EGD-e Listeria monocytogenes EGD-e]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5ZQD OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5ZQD FirstGlance]. <br> |
| - | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CEF:CEFOTAXIME,+C3+CLEAVED,+OPEN,+BOUND+FORM'>CEF</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.888Å</td></tr> |
| - | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">lmo2812 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=169963 LISMO])</td></tr> | + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CEF:CEFOTAXIME,+C3+CLEAVED,+OPEN,+BOUND+FORM'>CEF</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene></td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5zqd FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5zqd OCA], [http://pdbe.org/5zqd PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5zqd RCSB], [http://www.ebi.ac.uk/pdbsum/5zqd PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5zqd ProSAT]</span></td></tr> | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5zqd FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5zqd OCA], [https://pdbe.org/5zqd PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5zqd RCSB], [https://www.ebi.ac.uk/pdbsum/5zqd PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5zqd ProSAT]</span></td></tr> |
| | </table> | | </table> |
| | + | == Function == |
| | + | [https://www.uniprot.org/uniprot/Q8Y3M3_LISMO Q8Y3M3_LISMO] |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| | == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
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| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Lismo]] | + | [[Category: Large Structures]] |
| - | [[Category: Jeong, J H]] | + | [[Category: Listeria monocytogenes EGD-e]] |
| - | [[Category: Kim, Y G]]
| + | [[Category: Jeong JH]] |
| - | [[Category: Antibiotic]]
| + | [[Category: Kim YG]] |
| - | [[Category: Beta-lactam]]
| + | |
| - | [[Category: Dd-carboxypeptidase]] | + | |
| - | [[Category: Lmpbpd2]] | + | |
| Structural highlights
Function
Q8Y3M3_LISMO
Publication Abstract from PubMed
beta-lactam antibiotics that inhibit penicillin-binding proteins (PBPs) have been widely used in the treatment of bacterial infections. However, the molecular basis underlying the different inhibitory potencies of beta-lactams against specific PBPs is not fully understood. Here, we present the crystal structures of PBPD2 from Listeria monocytogenes, a Gram-positive food-borne bacterial pathogen that causes listeriosis in humans. The acylated structures in complex with four antibiotics (penicillin G, ampicillin, cefotaxime, and cefuroxime) revealed that the beta-lactam core structures were recognized by a common set of residues; however, the R1 side chains of each antibiotic participate in different interactions with PBPD2. In addition, the structural complementarities between the side chains of beta-lactams and the enzyme were found to be highly correlated with the relative reactivities of penam or cephem antibiotics against PBPD2. Our study provides the structural basis for the inhibition of PBPD2 by clinically important beta-lactam antibiotics that are commonly used in listeriosis treatment. Our findings imply that the modification of beta-lactam side chains based on structural complementarity could be useful for the development of potent inhibitors against beta-lactam-resistant PBPs.
Crystal structures of penicillin-binding protein D2 from Listeria monocytogenes: Structural basis for antibiotic specificity.,Jeong JH, Cha HJ, Kim YG Antimicrob Agents Chemother. 2018 Aug 6. pii: AAC.00796-18. doi:, 10.1128/AAC.00796-18. PMID:30082290[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Jeong JH, Cha HJ, Kim YG. Crystal structures of penicillin-binding protein D2 from Listeria monocytogenes: Structural basis for antibiotic specificity. Antimicrob Agents Chemother. 2018 Aug 6. pii: AAC.00796-18. doi:, 10.1128/AAC.00796-18. PMID:30082290 doi:http://dx.doi.org/10.1128/AAC.00796-18
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