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| | <StructureSection load='5zqh' size='340' side='right'caption='[[5zqh]], [[Resolution|resolution]] 2.40Å' scene=''> | | <StructureSection load='5zqh' size='340' side='right'caption='[[5zqh]], [[Resolution|resolution]] 2.40Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[5zqh]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/"diplococcus_pneumoniae"_(klein_1884)_weichselbaum_1886 "diplococcus pneumoniae" (klein 1884) weichselbaum 1886]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5ZQH OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=5ZQH FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[5zqh]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Streptococcus_pneumoniae Streptococcus pneumoniae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5ZQH OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5ZQH FirstGlance]. <br> |
| - | </td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=5zqh FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5zqh OCA], [http://pdbe.org/5zqh PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5zqh RCSB], [http://www.ebi.ac.uk/pdbsum/5zqh PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5zqh ProSAT]</span></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.4Å</td></tr> |
| | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5zqh FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5zqh OCA], [https://pdbe.org/5zqh PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5zqh RCSB], [https://www.ebi.ac.uk/pdbsum/5zqh PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5zqh ProSAT]</span></td></tr> |
| | </table> | | </table> |
| | + | == Function == |
| | + | [https://www.uniprot.org/uniprot/A5M9R0_STREE A5M9R0_STREE] |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| | == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
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| | </div> | | </div> |
| | <div class="pdbe-citations 5zqh" style="background-color:#fffaf0;"></div> | | <div class="pdbe-citations 5zqh" style="background-color:#fffaf0;"></div> |
| | + | |
| | + | ==See Also== |
| | + | *[[Transcriptional activator 3D structures|Transcriptional activator 3D structures]] |
| | == References == | | == References == |
| | <references/> | | <references/> |
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| | </StructureSection> | | </StructureSection> |
| | [[Category: Large Structures]] | | [[Category: Large Structures]] |
| - | [[Category: Hong, M]] | + | [[Category: Streptococcus pneumoniae]] |
| - | [[Category: Kim, M]] | + | [[Category: Hong M]] |
| - | [[Category: Antibiotic resistance]] | + | [[Category: Kim M]] |
| - | [[Category: Bacteria]]
| + | |
| - | [[Category: Dna binding protein]]
| + | |
| - | [[Category: Padr]]
| + | |
| - | [[Category: Regulator]]
| + | |
| - | [[Category: Transcription]]
| + | |
| Structural highlights
Function
A5M9R0_STREE
Publication Abstract from PubMed
Since the first discovery of phenolic acid decarboxylase transcriptional regulator (PadR), its homologs have been identified mostly in bacterial species and constitute the PadR family. PadR family members commonly contain a winged helix-turn-helix (wHTH) motif and function as a transcription factor. However, the PadR family members are varied in terms of molecular size and structure. As a result, they are divided into PadR subfamily-1 and PadR subfamily-2. PadR subfamily-2 proteins have been reported in some pathogenic bacteria, including Listeria monocytogenes and Streptococcus pneumoniae, and implicated in drug resistance processes. Despite the growing numbers of known PadR family proteins and their critical functions in bacteria survival, biochemical and biophysical studies of the PadR subfamily-2 are limited. Here, we report the crystal structure of a PadR subfamily-2 member from Streptococcus pneumoniae (SpPadR) at a 2.40 A resolution. SpPadR forms a dimer using its N-terminal and C-terminal helices. The two wHTH motifs of a SpPadR dimer expose their positively charged residues presumably to interact with DNA. Our structure-based mutational and biochemical study indicates that SpPadR specifically recognizes a palindromic nucleotide sequence upstream of its encoding region as a transcriptional regulator. Furthermore, comparative structural analysis of diverse PadR family members combined with a modeling study highlights the structural and regulatory features of SpPadR that are canonical to the PadR family or specific to the PadR subfamily-2.
Structure-based functional analysis of a PadR transcription factor from Streptococcus pneumoniae and characteristic features in the PadR subfamily-2.,Lee C, Kim MI, Park J, Oh H, Kim J, Hong J, Jeon BY, Ka H, Hong M Biochem Biophys Res Commun. 2020 Nov 5;532(2):251-257. doi:, 10.1016/j.bbrc.2020.08.035. Epub 2020 Aug 28. PMID:32868077[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
See Also
References
- ↑ Lee C, Kim MI, Park J, Oh H, Kim J, Hong J, Jeon BY, Ka H, Hong M. Structure-based functional analysis of a PadR transcription factor from Streptococcus pneumoniae and characteristic features in the PadR subfamily-2. Biochem Biophys Res Commun. 2020 Nov 5;532(2):251-257. doi:, 10.1016/j.bbrc.2020.08.035. Epub 2020 Aug 28. PMID:32868077 doi:http://dx.doi.org/10.1016/j.bbrc.2020.08.035
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