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| | <StructureSection load='5zuu' size='340' side='right'caption='[[5zuu]], [[Resolution|resolution]] 1.95Å' scene=''> | | <StructureSection load='5zuu' size='340' side='right'caption='[[5zuu]], [[Resolution|resolution]] 1.95Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[5zuu]] is a 8 chain structure with sequence from [http://en.wikipedia.org/wiki/Arath Arath]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5ZUU OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5ZUU FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[5zuu]] is a 8 chain structure with sequence from [https://en.wikipedia.org/wiki/Arabidopsis_thaliana Arabidopsis thaliana] and [https://en.wikipedia.org/wiki/Synthetic_construct Synthetic construct]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5ZUU OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5ZUU FirstGlance]. <br> |
| - | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=EPE:4-(2-HYDROXYETHYL)-1-PIPERAZINE+ETHANESULFONIC+ACID'>EPE</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.95Å</td></tr> |
| - | <tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=6MD:N-METHYLADENOSINE'>6MD</scene></td></tr> | + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=6MD:N-METHYLADENOSINE'>6MD</scene>, <scene name='pdbligand=EPE:4-(2-HYDROXYETHYL)-1-PIPERAZINE+ETHANESULFONIC+ACID'>EPE</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene></td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5zuu FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5zuu OCA], [http://pdbe.org/5zuu PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5zuu RCSB], [http://www.ebi.ac.uk/pdbsum/5zuu PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5zuu ProSAT]</span></td></tr> | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5zuu FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5zuu OCA], [https://pdbe.org/5zuu PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5zuu RCSB], [https://www.ebi.ac.uk/pdbsum/5zuu PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5zuu ProSAT]</span></td></tr> |
| | </table> | | </table> |
| | == Function == | | == Function == |
| - | [[http://www.uniprot.org/uniprot/CPSF_ARATH CPSF_ARATH]] Component of the cleavage and polyadenylation specificity factor (CPSF) complex that play a key role in pre-mRNA 3'-end formation. May interact with poly(A) polymerase and other factors to bring about cleavage and poly(A) addition (By similarity). Mediates poly(A) site selection (PubMed:23136375). Binds RNA in a calcium-dependent manner (PubMed:16500995, PubMed:17576667, PubMed:20214900). Exhibits endonuclease activity with an ability to nick and degrade linear as well as circular single-stranded RNA that leaves RNA 3' ends with hydroxyl groups, thus mediating processing of the pre-mRNA as a prelude to the polyadenylation (PubMed:17576667). Involved in the post-transcriptional control, probably via poly(A) addition, of the responses of plants to stress, especially genes mediating tolerance to oxidative stress (PubMed:18545667). Plays a role in the regulation of salicylic acid (SA) production via the control of messenger RNA 3' end processing, thus being a key component of programmed cell death and plant immune responses required for resistance to virulent Pseudomonas syringae pv tomato DC3000 (Pst) (PubMed:24706550).[UniProtKB:O95639]<ref>PMID:16500995</ref> <ref>PMID:17576667</ref> <ref>PMID:18545667</ref> <ref>PMID:20214900</ref> <ref>PMID:23136375</ref> <ref>PMID:24706550</ref> | + | [https://www.uniprot.org/uniprot/CPSF_ARATH CPSF_ARATH] Component of the cleavage and polyadenylation specificity factor (CPSF) complex that play a key role in pre-mRNA 3'-end formation. May interact with poly(A) polymerase and other factors to bring about cleavage and poly(A) addition (By similarity). Mediates poly(A) site selection (PubMed:23136375). Binds RNA in a calcium-dependent manner (PubMed:16500995, PubMed:17576667, PubMed:20214900). Exhibits endonuclease activity with an ability to nick and degrade linear as well as circular single-stranded RNA that leaves RNA 3' ends with hydroxyl groups, thus mediating processing of the pre-mRNA as a prelude to the polyadenylation (PubMed:17576667). Involved in the post-transcriptional control, probably via poly(A) addition, of the responses of plants to stress, especially genes mediating tolerance to oxidative stress (PubMed:18545667). Plays a role in the regulation of salicylic acid (SA) production via the control of messenger RNA 3' end processing, thus being a key component of programmed cell death and plant immune responses required for resistance to virulent Pseudomonas syringae pv tomato DC3000 (Pst) (PubMed:24706550).[UniProtKB:O95639]<ref>PMID:16500995</ref> <ref>PMID:17576667</ref> <ref>PMID:18545667</ref> <ref>PMID:20214900</ref> <ref>PMID:23136375</ref> <ref>PMID:24706550</ref> |
| | == References == | | == References == |
| | <references/> | | <references/> |
| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Arath]] | + | [[Category: Arabidopsis thaliana]] |
| | [[Category: Large Structures]] | | [[Category: Large Structures]] |
| - | [[Category: Li, S S]] | + | [[Category: Synthetic construct]] |
| - | [[Category: Nie, H B]] | + | [[Category: Li S]] |
| - | [[Category: Patel, D J]] | + | [[Category: Nie H]] |
| - | [[Category: Wu, B X]] | + | [[Category: Patel DJ]] |
| - | [[Category: Arabidopsis]]
| + | [[Category: Wu B]] |
| - | [[Category: Cpsf]]
| + | |
| - | [[Category: Modification]]
| + | |
| - | [[Category: Mrna processing]]
| + | |
| - | [[Category: Rna binding protein-rna complex]]
| + | |
| Structural highlights
Function
CPSF_ARATH Component of the cleavage and polyadenylation specificity factor (CPSF) complex that play a key role in pre-mRNA 3'-end formation. May interact with poly(A) polymerase and other factors to bring about cleavage and poly(A) addition (By similarity). Mediates poly(A) site selection (PubMed:23136375). Binds RNA in a calcium-dependent manner (PubMed:16500995, PubMed:17576667, PubMed:20214900). Exhibits endonuclease activity with an ability to nick and degrade linear as well as circular single-stranded RNA that leaves RNA 3' ends with hydroxyl groups, thus mediating processing of the pre-mRNA as a prelude to the polyadenylation (PubMed:17576667). Involved in the post-transcriptional control, probably via poly(A) addition, of the responses of plants to stress, especially genes mediating tolerance to oxidative stress (PubMed:18545667). Plays a role in the regulation of salicylic acid (SA) production via the control of messenger RNA 3' end processing, thus being a key component of programmed cell death and plant immune responses required for resistance to virulent Pseudomonas syringae pv tomato DC3000 (Pst) (PubMed:24706550).[UniProtKB:O95639][1] [2] [3] [4] [5] [6]
References
- ↑ Delaney KJ, Xu R, Zhang J, Li QQ, Yun KY, Falcone DL, Hunt AG. Calmodulin interacts with and regulates the RNA-binding activity of an Arabidopsis polyadenylation factor subunit. Plant Physiol. 2006 Apr;140(4):1507-21. doi: 10.1104/pp.105.070672. Epub 2006 Feb, 24. PMID:16500995 doi:http://dx.doi.org/10.1104/pp.105.070672
- ↑ Addepalli B, Hunt AG. A novel endonuclease activity associated with the Arabidopsis ortholog of the 30-kDa subunit of cleavage and polyadenylation specificity factor. Nucleic Acids Res. 2007;35(13):4453-63. doi: 10.1093/nar/gkm457. Epub 2007 Jun, 18. PMID:17576667 doi:http://dx.doi.org/10.1093/nar/gkm457
- ↑ Zhang J, Addepalli B, Yun KY, Hunt AG, Xu R, Rao S, Li QQ, Falcone DL. A polyadenylation factor subunit implicated in regulating oxidative signaling in Arabidopsis thaliana. PLoS One. 2008 Jun 11;3(6):e2410. doi: 10.1371/journal.pone.0002410. PMID:18545667 doi:http://dx.doi.org/10.1371/journal.pone.0002410
- ↑ Bell SA, Hunt AG. The Arabidopsis ortholog of the 77 kDa subunit of the cleavage stimulatory factor (AtCstF-77) involved in mRNA polyadenylation is an RNA-binding protein. FEBS Lett. 2010 Apr 16;584(8):1449-54. doi: 10.1016/j.febslet.2010.03.007. Epub, 2010 Mar 6. PMID:20214900 doi:http://dx.doi.org/10.1016/j.febslet.2010.03.007
- ↑ Thomas PE, Wu X, Liu M, Gaffney B, Ji G, Li QQ, Hunt AG. Genome-wide control of polyadenylation site choice by CPSF30 in Arabidopsis. Plant Cell. 2012 Nov;24(11):4376-88. doi: 10.1105/tpc.112.096107. Epub 2012 Nov, 6. PMID:23136375 doi:http://dx.doi.org/10.1105/tpc.112.096107
- ↑ Bruggeman Q, Garmier M, de Bont L, Soubigou-Taconnat L, Mazubert C, Benhamed M, Raynaud C, Bergounioux C, Delarue M. The Polyadenylation Factor Subunit CLEAVAGE AND POLYADENYLATION SPECIFICITY FACTOR30: A Key Factor of Programmed Cell Death and a Regulator of Immunity in Arabidopsis. Plant Physiol. 2014 Jun;165(2):732-746. doi: 10.1104/pp.114.236083. Epub 2014 Apr, 4. PMID:24706550 doi:http://dx.doi.org/10.1104/pp.114.236083
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