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| | <StructureSection load='6a2h' size='340' side='right'caption='[[6a2h]], [[Resolution|resolution]] 2.30Å' scene=''> | | <StructureSection load='6a2h' size='340' side='right'caption='[[6a2h]], [[Resolution|resolution]] 2.30Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[6a2h]] is a 3 chain structure with sequence from [http://en.wikipedia.org/wiki/Sacs2 Sacs2]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6A2H OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6A2H FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[6a2h]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Saccharolobus_solfataricus_P2 Saccharolobus solfataricus P2] and [https://en.wikipedia.org/wiki/Synthetic_construct Synthetic construct]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6A2H OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6A2H FirstGlance]. <br> |
| - | </td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">creN7, SSO6901 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=273057 SACS2])</td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.3Å</td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6a2h FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6a2h OCA], [http://pdbe.org/6a2h PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6a2h RCSB], [http://www.ebi.ac.uk/pdbsum/6a2h PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6a2h ProSAT]</span></td></tr> | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6a2h FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6a2h OCA], [https://pdbe.org/6a2h PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6a2h RCSB], [https://www.ebi.ac.uk/pdbsum/6a2h PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6a2h ProSAT]</span></td></tr> |
| | </table> | | </table> |
| | == Function == | | == Function == |
| - | [[http://www.uniprot.org/uniprot/CREN7_SULSO CREN7_SULSO]] A highly abundant probable chromatin protein, it binds double-strand DNA without sequence specificity; there is approximately 1 Cren7 molecule for 12 bp of DNA. Constrains negative DNA supercoils, increases DNA stability against thermal denaturation. Binding does not require protein methylation. Binds single-strand DNA weakly.<ref>PMID:18096617</ref> | + | [https://www.uniprot.org/uniprot/CREN7_SACS2 CREN7_SACS2] A highly abundant probable chromatin protein, it binds double-strand DNA without sequence specificity; there is approximately 1 Cren7 molecule for 12 bp of DNA. Constrains negative DNA supercoils, increases DNA stability against thermal denaturation. Binding does not require protein methylation. Binds single-strand DNA weakly.<ref>PMID:18096617</ref> |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| | == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
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| | </StructureSection> | | </StructureSection> |
| | [[Category: Large Structures]] | | [[Category: Large Structures]] |
| - | [[Category: Sacs2]] | + | [[Category: Saccharolobus solfataricus P2]] |
| - | [[Category: Chen, Y Y]] | + | [[Category: Synthetic construct]] |
| - | [[Category: Dong, Y H]] | + | [[Category: Chen YY]] |
| - | [[Category: Gong, Y]] | + | [[Category: Dong YH]] |
| - | [[Category: Huang, L]] | + | [[Category: Gong Y]] |
| - | [[Category: Wang, L]] | + | [[Category: Huang L]] |
| - | [[Category: Zhang, Z F]] | + | [[Category: Wang L]] |
| - | [[Category: Zhao, M H]] | + | [[Category: Zhang ZF]] |
| - | [[Category: Beta-sheet]]
| + | [[Category: Zhao MH]] |
| - | [[Category: Crenarchaeal chromatin protein]]
| + | |
| - | [[Category: Dna binding protein-dna complex]]
| + | |
| Structural highlights
Function
CREN7_SACS2 A highly abundant probable chromatin protein, it binds double-strand DNA without sequence specificity; there is approximately 1 Cren7 molecule for 12 bp of DNA. Constrains negative DNA supercoils, increases DNA stability against thermal denaturation. Binding does not require protein methylation. Binds single-strand DNA weakly.[1]
Publication Abstract from PubMed
Archaea have evolved various strategies in chromosomal organization. While histone homologues exist in most archaeal phyla, Cren7 is a chromatin protein conserved in the Crenarchaeota. Here, we show that Cren7 preferentially binds DNA with AT-rich sequences over that with GC-rich sequences with a binding size of 6~7 bp. Structural studies of Cren7 in complex with either an 18-bp or a 20-bp double-stranded DNA fragment reveal that Cren7 binds to the minor groove of DNA as monomers in a head-to-tail manner. The neighboring Cren7 monomers are located on the opposite sides of the DNA duplex, with each introducing a single-step sharp kink by intercalation of the hydrophobic side chain of Leu28, bending the DNA into an S-shape conformation. A structural model for the chromatin fiber folded by Cren7 was established and verified by the analysis of cross-linked Cren7-DNA complexes by atomic force microscopy. Our results suggest that Cren7 differs significantly from Sul7, another chromatin protein conserved among Sulfolobus species, in both DNA binding and deformation. These data shed significant light on the strategy of chromosomal DNA organization in crenarchaea.
Architectural roles of Cren7 in folding crenarchaeal chromatin filament.,Zhang Z, Zhao M, Chen Y, Wang L, Liu Q, Dong Y, Gong Y, Huang L Mol Microbiol. 2018 Nov 30. doi: 10.1111/mmi.14173. PMID:30499242[2]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Guo L, Feng Y, Zhang Z, Yao H, Luo Y, Wang J, Huang L. Biochemical and structural characterization of Cren7, a novel chromatin protein conserved among Crenarchaea. Nucleic Acids Res. 2008 Mar;36(4):1129-37. Epub 2007 Dec 20. PMID:18096617 doi:10.1093/nar/gkm1128
- ↑ Zhang Z, Zhao M, Chen Y, Wang L, Liu Q, Dong Y, Gong Y, Huang L. Architectural roles of Cren7 in folding crenarchaeal chromatin filament. Mol Microbiol. 2018 Nov 30. doi: 10.1111/mmi.14173. PMID:30499242 doi:http://dx.doi.org/10.1111/mmi.14173
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