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| | <StructureSection load='6a2v' size='340' side='right'caption='[[6a2v]], [[Resolution|resolution]] 2.59Å' scene=''> | | <StructureSection load='6a2v' size='340' side='right'caption='[[6a2v]], [[Resolution|resolution]] 2.59Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[6a2v]] is a 6 chain structure with sequence from [http://en.wikipedia.org/wiki/Campylobacter_jejuni_jejuni Campylobacter jejuni jejuni]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6A2V OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=6A2V FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[6a2v]] is a 6 chain structure with sequence from [https://en.wikipedia.org/wiki/Campylobacter_jejuni_subsp._jejuni Campylobacter jejuni subsp. jejuni]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6A2V OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6A2V FirstGlance]. <br> |
| - | </td></tr><tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.588Å</td></tr> |
| - | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">CV369_03455 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=32022 Campylobacter jejuni jejuni])</td></tr> | + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=6a2v FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6a2v OCA], [http://pdbe.org/6a2v PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6a2v RCSB], [http://www.ebi.ac.uk/pdbsum/6a2v PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6a2v ProSAT]</span></td></tr> | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6a2v FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6a2v OCA], [https://pdbe.org/6a2v PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6a2v RCSB], [https://www.ebi.ac.uk/pdbsum/6a2v PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6a2v ProSAT]</span></td></tr> |
| | </table> | | </table> |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
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| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Campylobacter jejuni jejuni]] | + | [[Category: Campylobacter jejuni subsp. jejuni]] |
| | [[Category: Large Structures]] | | [[Category: Large Structures]] |
| - | [[Category: Jobichen, C]] | + | [[Category: Jobichen C]] |
| - | [[Category: Sivaraman, J]] | + | [[Category: Sivaraman J]] |
| - | [[Category: Hemolysin coregulated protein]]
| + | |
| - | [[Category: Structural protein]]
| + | |
| - | [[Category: Type vi secretion system protein]]
| + | |
| Structural highlights
Publication Abstract from PubMed
The Type VI Secretion System (T6SS) provides enhanced virulence to Campylobacter jejuni and has been associated with a high incidence of bloody diarrhea. The hemolysin-coregulated protein (Hcp)-the hallmark of the T6SS-can act as a structural and effector protein. Unlike other T6SS-harboring bacteria, which possess multiple Hcp proteins each performing different functions, C. jejuni possesses only one Hcp protein, and its structural and functional role has not been elucidated previously. Here, we report the structure and functional studies of Hcp from C. jejuni. We found similarities between the hexameric ring structure of Hcp-Cj and that of Hcp3 from Pseudomonas aeruginosa. Through functional studies, we showed two roles for Hcp-Cj i.e., in cytotoxicity towards HepG2 cells and in biofilm formation in C. jejuni. In structure-based mutational analyses, we showed that an Arg-to-Ala mutation at position 30 within the extended loop results in a significant decrease in cytotoxicity, suggesting a role for this loop in binding to the host cell. However, this mutation does not affect its biofilm formation function. Collectively, this study supports the dual role of Hcp-Cj as a structural and effector protein in C. jejuni. This article is protected by copyright. All rights reserved.
Structural basis for the pathogenesis of Campylobacter jejuni Hcp1, a structural and effector protein of the type six secretion system.,Noreen Z, Jobichen C, Abbasi R, Seetharaman J, Sivaraman J, Bohkhari H FEBS J. 2018 Sep 7. doi: 10.1111/febs.14650. PMID:30194714[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Noreen Z, Jobichen C, Abbasi R, Seetharaman J, Sivaraman J, Bohkhari H. Structural basis for the pathogenesis of Campylobacter jejuni Hcp1, a structural and effector protein of the type six secretion system. FEBS J. 2018 Sep 7. doi: 10.1111/febs.14650. PMID:30194714 doi:http://dx.doi.org/10.1111/febs.14650
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