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| | ==Structure of TREX2 in complex with a Y structured dsDNA== | | ==Structure of TREX2 in complex with a Y structured dsDNA== |
| - | <StructureSection load='6a47' size='340' side='right' caption='[[6a47]], [[Resolution|resolution]] 1.90Å' scene=''> | + | <StructureSection load='6a47' size='340' side='right'caption='[[6a47]], [[Resolution|resolution]] 1.90Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[6a47]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Lk3_transgenic_mice Lk3 transgenic mice]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6A47 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6A47 FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[6a47]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6A47 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6A47 FirstGlance]. <br> |
| - | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.9Å</td></tr> |
| - | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">Trex2 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10090 LK3 transgenic mice])</td></tr> | + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene></td></tr> |
| - | <tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Exodeoxyribonuclease_III Exodeoxyribonuclease III], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.11.2 3.1.11.2] </span></td></tr>
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6a47 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6a47 OCA], [https://pdbe.org/6a47 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6a47 RCSB], [https://www.ebi.ac.uk/pdbsum/6a47 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6a47 ProSAT]</span></td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6a47 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6a47 OCA], [http://pdbe.org/6a47 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6a47 RCSB], [http://www.ebi.ac.uk/pdbsum/6a47 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6a47 ProSAT]</span></td></tr> | + | |
| | </table> | | </table> |
| | + | == Function == |
| | + | [https://www.uniprot.org/uniprot/TREX2_MOUSE TREX2_MOUSE] |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| | == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
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| | </div> | | </div> |
| | <div class="pdbe-citations 6a47" style="background-color:#fffaf0;"></div> | | <div class="pdbe-citations 6a47" style="background-color:#fffaf0;"></div> |
| | + | |
| | + | ==See Also== |
| | + | *[[Exonuclease 3D structures|Exonuclease 3D structures]] |
| | == References == | | == References == |
| | <references/> | | <references/> |
| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Exodeoxyribonuclease III]] | + | [[Category: Large Structures]] |
| - | [[Category: Lk3 transgenic mice]] | + | [[Category: Mus musculus]] |
| - | [[Category: Hsiao, Y Y]] | + | [[Category: Hsiao YY]] |
| - | [[Category: Deddh exonuclease]]
| + | |
| - | [[Category: Dna binding protein]]
| + | |
| - | [[Category: Exonuclease]]
| + | |
| Structural highlights
Function
TREX2_MOUSE
Publication Abstract from PubMed
The three prime repair exonuclease 2 (TREX2) is an essential 3'-to-5' exonuclease that functions in cell proliferation, genome integrity and skin homeostasis maintenance. The abnormal expression level of TREX2 can result in broken chromosome, increased susceptibility to skin carcinogenesis and Psoriasis. However, the molecular mechanisms of how TREX2 binds and processes its natural substrates, dsDNA or chromosomal DNA, to maintain genome stability remain unclear. In this study, we present four new crystal structures: apo-TREX2, TREX2 in complex with two different dsDNA substrates, and TREX2 in complex with a processed dsDNA product. Analysis of the structures reveals that TREX2 stacks with the 5'-terminal of dsDNA by a Leu20-Pro21-Asn22 cluster for precisely trimming the 3'-overhang. In addition, TREX2 specifically interacts with the non-scissile strand of dsDNA by an alpha-helix-loop region. The unique interaction patterns of the TREX2-dsDNA complex highlight the requirement of long double-stranded region for TREX2 binding and provide evidence of the functional role of TREX2 in processing chromosomal DNA. Moreover, the non-processive property of TREX2 is elucidated by the structure of TREX2-product complex. Our work discloses the first structural basis of the molecular interactions between TREX2 and its substrates and unravels the mechanistic actions of TREX2.
Structural insights into the duplex DNA processing of TREX2.,Cheng HL, Lin CT, Huang KW, Wang S, Lin YT, Toh SI, Hsiao YY Nucleic Acids Res. 2018 Oct 24. pii: 5144137. doi: 10.1093/nar/gky970. PMID:30357414[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
See Also
References
- ↑ Cheng HL, Lin CT, Huang KW, Wang S, Lin YT, Toh SI, Hsiao YY. Structural insights into the duplex DNA processing of TREX2. Nucleic Acids Res. 2018 Oct 24. pii: 5144137. doi: 10.1093/nar/gky970. PMID:30357414 doi:http://dx.doi.org/10.1093/nar/gky970
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