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| | <StructureSection load='6a6w' size='340' side='right'caption='[[6a6w]], [[Resolution|resolution]] 2.60Å' scene=''> | | <StructureSection load='6a6w' size='340' side='right'caption='[[6a6w]], [[Resolution|resolution]] 2.60Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[6a6w]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Fission_yeast Fission yeast]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6A6W OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6A6W FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[6a6w]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Schizosaccharomyces_pombe_972h- Schizosaccharomyces pombe 972h-]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6A6W OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6A6W FirstGlance]. <br> |
| - | </td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[5ybx|5ybx]], [[5yc2|5yc2]], [[5yca|5yca]]</td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.601Å</td></tr> |
| - | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">bqt4, SPBC19C7.10 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=284812 Fission yeast]), sad1, SPBC12D12.01, SPBC16H5.01c ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=284812 Fission yeast])</td></tr>
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6a6w FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6a6w OCA], [https://pdbe.org/6a6w PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6a6w RCSB], [https://www.ebi.ac.uk/pdbsum/6a6w PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6a6w ProSAT]</span></td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6a6w FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6a6w OCA], [http://pdbe.org/6a6w PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6a6w RCSB], [http://www.ebi.ac.uk/pdbsum/6a6w PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6a6w ProSAT]</span></td></tr> | + | |
| | </table> | | </table> |
| | == Function == | | == Function == |
| - | [[http://www.uniprot.org/uniprot/BQT4_SCHPO BQT4_SCHPO]] Connects telomeres to the nuclear envelop (NE) during both vegetative growth and meiosis. This connection ensures clustering of telomeres to the spindle pole body (SPB) when cells enter meiotic prophase.<ref>PMID:19948484</ref> [[http://www.uniprot.org/uniprot/SAD1_SCHPO SAD1_SCHPO]] Associates with the spindle pole body and maintains a functional interface between the nuclear membrane and the microtubule motor proteins. Involved in chromosome segregation during meiosis where it associates with the telomeres.<ref>PMID:16615890</ref> | + | [https://www.uniprot.org/uniprot/BQT4_SCHPO BQT4_SCHPO] Connects telomeres to the nuclear envelop (NE) during both vegetative growth and meiosis. This connection ensures clustering of telomeres to the spindle pole body (SPB) when cells enter meiotic prophase.<ref>PMID:19948484</ref> |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| | == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
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| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Fission yeast]] | |
| | [[Category: Large Structures]] | | [[Category: Large Structures]] |
| - | [[Category: Chen, Y]] | + | [[Category: Schizosaccharomyces pombe 972h-]] |
| - | [[Category: Hu, C]] | + | [[Category: Chen Y]] |
| - | [[Category: Bouquet formation]] | + | [[Category: Hu C]] |
| - | [[Category: Chromosome organization]]
| + | |
| - | [[Category: Inner nuclear membrane protein]]
| + | |
| - | [[Category: Membrane protein]]
| + | |
| - | [[Category: Spindle pole boday]]
| + | |
| Structural highlights
Function
BQT4_SCHPO Connects telomeres to the nuclear envelop (NE) during both vegetative growth and meiosis. This connection ensures clustering of telomeres to the spindle pole body (SPB) when cells enter meiotic prophase.[1]
Publication Abstract from PubMed
The dynamic association of chromosomes with the nuclear envelope (NE) is essential for chromosome maintenance. Schizosaccharomyces pombe inner nuclear membrane protein Bqt4 plays a critical role in connecting telomeres to the NE, mainly through a direct interaction with the telomeric protein Rap1. Bqt4 also interacts with Lem2 for pericentric heterochromatin maintenance. How Bqt4 coordinates the interactions with different proteins to exert their functions is unclear. Here, we report the crystal structures of the N-terminal domain of Bqt4 in complexes with Bqt4-binding motifs from Rap1, Lem2, and Sad1. The structural, biochemical and cellular analyses reveal that the N-terminal domain of Bqt4 is a protein-interaction module that recognizes a consensus motif and plays essential roles in telomere-NE association and meiosis progression. Phosphorylation of Bqt4-interacting proteins may act as a switch to regulate these interactions during cell cycles. Our studies provide structural insights into the identification and regulation of Bqt4-mediated interactions.
Structural insights into chromosome attachment to the nuclear envelope by an inner nuclear membrane protein Bqt4 in fission yeast.,Hu C, Inoue H, Sun W, Takeshita Y, Huang Y, Xu Y, Kanoh J, Chen Y Nucleic Acids Res. 2018 Nov 20. pii: 5193554. doi: 10.1093/nar/gky1186. PMID:30462301[2]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Chikashige Y, Yamane M, Okamasa K, Tsutsumi C, Kojidani T, Sato M, Haraguchi T, Hiraoka Y. Membrane proteins Bqt3 and -4 anchor telomeres to the nuclear envelope to ensure chromosomal bouquet formation. J Cell Biol. 2009 Nov 2;187(3):413-27. doi: 10.1083/jcb.200902122. PMID:19948484 doi:http://dx.doi.org/10.1083/jcb.200902122
- ↑ Hu C, Inoue H, Sun W, Takeshita Y, Huang Y, Xu Y, Kanoh J, Chen Y. Structural insights into chromosome attachment to the nuclear envelope by an inner nuclear membrane protein Bqt4 in fission yeast. Nucleic Acids Res. 2018 Nov 20. pii: 5193554. doi: 10.1093/nar/gky1186. PMID:30462301 doi:http://dx.doi.org/10.1093/nar/gky1186
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