1oet

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[[Image:1oet.jpg|left|200px]]
[[Image:1oet.jpg|left|200px]]
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{{Structure
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<!--
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|PDB= 1oet |SIZE=350|CAPTION= <scene name='initialview01'>1oet</scene>, resolution 2.30&Aring;
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The line below this paragraph, containing "STRUCTURE_1oet", creates the "Structure Box" on the page.
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|SITE= <scene name='pdbsite=AC1:Mg+Binding+Site+For+Chain+A'>AC1</scene>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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|LIGAND= <scene name='pdbligand=CSO:S-HYDROXYCYSTEINE'>CSO</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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|ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/Protein-tyrosine-phosphatase Protein-tyrosine-phosphatase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.3.48 3.1.3.48] </span>
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or leave the SCENE parameter empty for the default display.
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|GENE=
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-->
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|DOMAIN=
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{{STRUCTURE_1oet| PDB=1oet | SCENE= }}
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|RELATEDENTRY=
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|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1oet FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1oet OCA], [http://www.ebi.ac.uk/pdbsum/1oet PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1oet RCSB]</span>
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}}
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'''OXIDATION STATE OF PROTEIN TYROSINE PHOSPHATASE 1B'''
'''OXIDATION STATE OF PROTEIN TYROSINE PHOSPHATASE 1B'''
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[[Category: Montfort, R L.M Van.]]
[[Category: Montfort, R L.M Van.]]
[[Category: Tisi, D.]]
[[Category: Tisi, D.]]
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[[Category: hydrolase,phosphorylation]]
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[[Category: Hydrolase,phosphorylation]]
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[[Category: oxidative regulation]]
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[[Category: Oxidative regulation]]
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[[Category: protein tyrosine phosphatase]]
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[[Category: Protein tyrosine phosphatase]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 03:45:20 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 22:44:16 2008''
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Revision as of 00:45, 3 May 2008

Image:1oet.jpg

Template:STRUCTURE 1oet

OXIDATION STATE OF PROTEIN TYROSINE PHOSPHATASE 1B


Overview

Protein tyrosine phosphatases regulate signal transduction pathways involving tyrosine phosphorylation and have been implicated in the development of cancer, diabetes, rheumatoid arthritis and hypertension. Increasing evidence suggests that the cellular redox state is involved in regulating tyrosine phosphatase activity through the reversible oxidization of the catalytic cysteine to sulphenic acid (Cys-SOH). But how further oxidation to the irreversible sulphinic (Cys-SO2H) and sulphonic (Cys-SO3H) forms is prevented remains unclear. Here we report the crystal structures of the regulatory sulphenic and irreversible sulphinic and sulphonic acids of protein tyrosine phosphatase 1B (PTP1B), an important enzyme in the negative regulation of the insulin receptor and a therapeutic target in type II diabetes and obesity. We also identify a sulphenyl-amide species that is formed through oxidation of its catalytic cysteine. Formation of the sulphenyl-amide causes large changes in the PTP1B active site, which are reversible by reduction with the cellular reducing agent glutathione. The sulphenyl-amide is a protective intermediate in the oxidative inhibition of PTP1B. In addition, it may facilitate reactivation of PTP1B by biological thiols and signal a unique state of the protein.

About this Structure

1OET is a Single protein structure of sequence from Homo sapiens. Full crystallographic information is available from OCA.

Reference

Oxidation state of the active-site cysteine in protein tyrosine phosphatase 1B., van Montfort RL, Congreve M, Tisi D, Carr R, Jhoti H, Nature. 2003 Jun 12;423(6941):773-7. PMID:12802339 Page seeded by OCA on Sat May 3 03:45:20 2008

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