|
|
| Line 3: |
Line 3: |
| | <StructureSection load='6ioy' size='340' side='right'caption='[[6ioy]], [[Resolution|resolution]] 1.94Å' scene=''> | | <StructureSection load='6ioy' size='340' side='right'caption='[[6ioy]], [[Resolution|resolution]] 1.94Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[6ioy]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Porg3 Porg3]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6IOY OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6IOY FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[6ioy]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Porphyromonas_gingivalis_ATCC_33277 Porphyromonas gingivalis ATCC 33277]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6IOY OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6IOY FirstGlance]. <br> |
| - | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=MPD:(4S)-2-METHYL-2,4-PENTANEDIOL'>MPD</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.94Å</td></tr> |
| - | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">ackA, PGN_1178 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=431947 PORG3])</td></tr> | + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MPD:(4S)-2-METHYL-2,4-PENTANEDIOL'>MPD</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr> |
| - | <tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Acetate_kinase Acetate kinase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.2.1 2.7.2.1] </span></td></tr>
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6ioy FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6ioy OCA], [https://pdbe.org/6ioy PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6ioy RCSB], [https://www.ebi.ac.uk/pdbsum/6ioy PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6ioy ProSAT]</span></td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6ioy FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6ioy OCA], [http://pdbe.org/6ioy PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6ioy RCSB], [http://www.ebi.ac.uk/pdbsum/6ioy PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6ioy ProSAT]</span></td></tr> | + | |
| | </table> | | </table> |
| | == Function == | | == Function == |
| - | [[http://www.uniprot.org/uniprot/ACKA_PORG3 ACKA_PORG3]] Catalyzes the formation of acetyl phosphate from acetate and ATP. Can also catalyze the reverse reaction. | + | [https://www.uniprot.org/uniprot/ACKA_PORG3 ACKA_PORG3] Catalyzes the formation of acetyl phosphate from acetate and ATP. Can also catalyze the reverse reaction. |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| | == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
| Line 24: |
Line 23: |
| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Acetate kinase]] | |
| | [[Category: Large Structures]] | | [[Category: Large Structures]] |
| - | [[Category: Porg3]] | + | [[Category: Porphyromonas gingivalis ATCC 33277]] |
| - | [[Category: Kezuka, Y]] | + | [[Category: Kezuka Y]] |
| - | [[Category: Nonaka, T]] | + | [[Category: Nonaka T]] |
| - | [[Category: Yoshida, Y]] | + | [[Category: Yoshida Y]] |
| - | [[Category: Atp]]
| + | |
| - | [[Category: Essential gene]]
| + | |
| - | [[Category: Porphyromonas gingivali]]
| + | |
| - | [[Category: Transferase]]
| + | |
| Structural highlights
Function
ACKA_PORG3 Catalyzes the formation of acetyl phosphate from acetate and ATP. Can also catalyze the reverse reaction.
Publication Abstract from PubMed
Acetyl phosphate (AcP) is generally produced from acetyl coenzyme A by phosphotransacetylase (Pta), and subsequent reaction with ADP, catalyzed by acetate kinase (Ack), produces ATP. The mechanism of ATP production in Porphyromonas gingivalis is poorly understood. The aim of this study was to explore the molecular basis of the Pta-Ack pathway in this microorganism. Pta and Ack from P. gingivalis ATCC 33277 were enzymatically and structurally characterized. Structural and mutational analyses suggest that Pta is a dimer with two substrate-binding sites in each subunit. Ack is also dimeric, with a catalytic cleft in each subunit, and structural analysis indicates a dramatic domain motion that opens and closes the cleft during catalysis. ATP formation by Ack proceeds via a sequential mechanism. Reverse transcription-PCR analysis demonstrated that the pta (PGN_1179) and ack (PGN_1178) genes, tandemly located in the genome, are cotranscribed as an operon. Inactivation of pta or ack in P. gingivalis by homologous recombination was successful only when the inactivated gene was expressed in trans. Therefore, both pta and ack genes are essential for this microorganism. Insights into the Pta-Ack pathway reported herein would be helpful to understand the energy acquisition in P. gingivalis.
Characterization of the phosphotransacetylase-acetate kinase pathway for ATP production in Porphyromonas gingivalis.,Yoshida Y, Sato M, Nonaka T, Hasegawa Y, Kezuka Y J Oral Microbiol. 2019 Apr 4;11(1):1588086. doi: 10.1080/20002297.2019.1588086., eCollection 2019. PMID:31007866[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Yoshida Y, Sato M, Nonaka T, Hasegawa Y, Kezuka Y. Characterization of the phosphotransacetylase-acetate kinase pathway for ATP production in Porphyromonas gingivalis. J Oral Microbiol. 2019 Apr 4;11(1):1588086. doi: 10.1080/20002297.2019.1588086., eCollection 2019. PMID:31007866 doi:http://dx.doi.org/10.1080/20002297.2019.1588086
|
