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| | <StructureSection load='6ive' size='340' side='right'caption='[[6ive]], [[Resolution|resolution]] 2.30Å' scene=''> | | <StructureSection load='6ive' size='340' side='right'caption='[[6ive]], [[Resolution|resolution]] 2.30Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[6ive]] is a 6 chain structure with sequence from [http://en.wikipedia.org/wiki/Thet8 Thet8]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6IVE OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6IVE FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[6ive]] is a 6 chain structure with sequence from [https://en.wikipedia.org/wiki/Thermus_thermophilus_HB8 Thermus thermophilus HB8]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6IVE OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6IVE FirstGlance]. <br> |
| - | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.3Å</td></tr> |
| - | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">TTHA1879 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=300852 THET8])</td></tr> | + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6ive FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6ive OCA], [http://pdbe.org/6ive PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6ive RCSB], [http://www.ebi.ac.uk/pdbsum/6ive PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6ive ProSAT]</span></td></tr> | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6ive FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6ive OCA], [https://pdbe.org/6ive PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6ive RCSB], [https://www.ebi.ac.uk/pdbsum/6ive PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6ive ProSAT]</span></td></tr> |
| | </table> | | </table> |
| | + | == Function == |
| | + | [https://www.uniprot.org/uniprot/Q5SH51_THET8 Q5SH51_THET8] |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| | == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
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| | </StructureSection> | | </StructureSection> |
| | [[Category: Large Structures]] | | [[Category: Large Structures]] |
| - | [[Category: Thet8]] | + | [[Category: Thermus thermophilus HB8]] |
| - | [[Category: Wang, H F]] | + | [[Category: Wang HF]] |
| - | [[Category: Wang, W M]] | + | [[Category: Wang WM]] |
| - | [[Category: Gamma-class carbonic anhydrase metal binding protein]]
| + | |
| - | [[Category: Metal binding protein]]
| + | |
| Structural highlights
Function
Q5SH51_THET8
Publication Abstract from PubMed
The gamma-class carbonic anhydrases (gamma-CAs) mainly come from methanogens methane-producing bacteria that grow in hot springs and catalyze the interconversion of carbon dioxide and water to bicarbonate and protons. Here, the gamma-CA from Thermus thermophilus HB8 (gamma-TtCA) was expressed and purified, its crystal structure was determined at 2.3 A resolution in space group P1. The asymmetric unit contains two trimers and six catalytic Zn(2+). In general, the fold of the protein is similar to those of homologous enzymes from Geobacillus Kaustophilus, Bacillus Cereus, Methanosarcina Thermophila and others. Each monomer comprises a triangular prism-like structure consisting of a left-handed beta-helix and a C-terminal alpha-helix. The catalytic Zn(2+) bound to three histidines and a phosphate radical in a tetrahedral fashion. It is located at the interface between the two monomers. Inductively coupled plasma mass spectrometry measurements further suggest that the molar ratio of zinc ions and protein molecules is 1:1. The structure revealed a novel different region situated between the left-handed beta-helix and the C-terminal alpha-helix. Compared to previously reported structures, half of the C-terminal alpha-helix was replaced with a long loop in this structure. The purified gamma-TtCA exhibits no significant carbonic anhydrase activity compared to alpha-class carbonic anhydrases. This study provides insight into the structural diversity of gamma-CAs with potential function for gamma-CAs.
Molecular structure of thermostable and zinc-ion-binding gamma-class carbonic anhydrases.,Wang W, Zhang Y, Wang L, Jing Q, Wang X, Xi X, Zhao X, Wang H Biometals. 2019 Apr;32(2):317-328. doi: 10.1007/s10534-019-00190-8. Epub 2019 Mar, 20. PMID:30895492[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Wang W, Zhang Y, Wang L, Jing Q, Wang X, Xi X, Zhao X, Wang H. Molecular structure of thermostable and zinc-ion-binding gamma-class carbonic anhydrases. Biometals. 2019 Apr;32(2):317-328. doi: 10.1007/s10534-019-00190-8. Epub 2019 Mar, 20. PMID:30895492 doi:http://dx.doi.org/10.1007/s10534-019-00190-8
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