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| | <StructureSection load='6j0y' size='340' side='right'caption='[[6j0y]], [[Resolution|resolution]] 1.80Å' scene=''> | | <StructureSection load='6j0y' size='340' side='right'caption='[[6j0y]], [[Resolution|resolution]] 1.80Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[6j0y]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Baker's_yeast Baker's yeast]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6J0Y OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6J0Y FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[6j0y]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Saccharomyces_cerevisiae_S288C Saccharomyces cerevisiae S288C]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6J0Y OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6J0Y FirstGlance]. <br> |
| - | </td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[6j0w|6j0w]]</td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.8Å</td></tr> |
| - | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">RTT107, ESC4, YHR154W ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=559292 Baker's yeast]), SLX4, YLR135W, L3140 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=559292 Baker's yeast])</td></tr>
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6j0y FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6j0y OCA], [https://pdbe.org/6j0y PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6j0y RCSB], [https://www.ebi.ac.uk/pdbsum/6j0y PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6j0y ProSAT]</span></td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6j0y FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6j0y OCA], [http://pdbe.org/6j0y PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6j0y RCSB], [http://www.ebi.ac.uk/pdbsum/6j0y PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6j0y ProSAT]</span></td></tr> | + | |
| | </table> | | </table> |
| | == Function == | | == Function == |
| - | [[http://www.uniprot.org/uniprot/RT107_YEAST RT107_YEAST]] Required for resumption of chromosome replication after DNA damage, specifically in S phase. Is recruited to chromatin in the presence of RTT109 and RTT101 in response to stalled replication forks and acts as a scaffold during DNA repair.<ref>PMID:14988729</ref> <ref>PMID:17978089</ref> [[http://www.uniprot.org/uniprot/SLX4_YEAST SLX4_YEAST]] Regulatory subunit that interacts with and increases the activity of different structure-specific endonucleases. Has several distinct roles in protecting genome stability by resolving diverse forms of deleterious DNA structures. Component of the SLX1-SLX4 structure-specific endonuclease that resolves DNA secondary structures generated during DNA repair and recombination. Has endonuclease activity towards branched DNA substrates, introducing single-strand cuts in duplex DNA close to junctions with ss-DNA. Has a preference for simple Y, 5'-flap and replication fork-like structures. It cleaves the strand bearing the 5'-non-homologous arm at the branch site junction and generates ligatable, nicked products from the 5'-flap or replication fork substrates. Plays a critical role in maintaining the integrity of the ribosomal DNA (rDNA) loci, where it has a role in re-starting stalled replication forks. Has Holliday junction resolvase activity in vitro. Interacts with the structure-specific RAD1-RAD10 endonuclease and promotes RAD1-RAD10-dependent 3'-non-homologous tail removal (NHTR) during repair of double-strand breaks by single-strand annealing. SLX4 also promotes recovery from DNA-alkylation-induced replisome stalling during DNA replication by facilitating the error-free mode of lesion bypass. This does not require SLX1 or RAD1-RAD10, but probably RTT107.[HAMAP-Rule:MF_03110]<ref>PMID:11139495</ref> <ref>PMID:12228808</ref> <ref>PMID:12832395</ref> <ref>PMID:15834151</ref> <ref>PMID:15975089</ref> <ref>PMID:16267268</ref> <ref>PMID:17636031</ref> <ref>PMID:18471978</ref> <ref>PMID:18579504</ref> | + | [https://www.uniprot.org/uniprot/RT107_YEAST RT107_YEAST] Required for resumption of chromosome replication after DNA damage, specifically in S phase. Is recruited to chromatin in the presence of RTT109 and RTT101 in response to stalled replication forks and acts as a scaffold during DNA repair.<ref>PMID:14988729</ref> <ref>PMID:17978089</ref> |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| | == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
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| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Baker's yeast]] | |
| | [[Category: Large Structures]] | | [[Category: Large Structures]] |
| - | [[Category: Lei, M]] | + | [[Category: Saccharomyces cerevisiae S288C]] |
| - | [[Category: Wan, B]] | + | [[Category: Lei M]] |
| - | [[Category: Wu, J]] | + | [[Category: Wan B]] |
| - | [[Category: Brct domain]] | + | [[Category: Wu J]] |
| - | [[Category: Mitosis]]
| + | |
| - | [[Category: Protein binding]]
| + | |
| - | [[Category: Protein interaction]]
| + | |
| Structural highlights
Function
RT107_YEAST Required for resumption of chromosome replication after DNA damage, specifically in S phase. Is recruited to chromatin in the presence of RTT109 and RTT101 in response to stalled replication forks and acts as a scaffold during DNA repair.[1] [2]
Publication Abstract from PubMed
BRCT domains support myriad protein-protein interactions involved in genome maintenance. Although di-BRCT recognition of phospho-proteins is well known to support the genotoxic response, whether multi-BRCT domains can acquire distinct structures and functions is unclear. Here we present the tetra-BRCT structures from the conserved yeast protein Rtt107 in free and ligand-bound forms. The four BRCT repeats fold into a tetrahedral structure that recognizes unmodified ligands using a bi-partite mechanism, suggesting repeat origami enabling function acquisition. Functional studies show that Rtt107 binding of partner proteins of diverse activities promotes genome replication and stability in both distinct and concerted manners. A unified theme is that tetra- and di-BRCT domains of Rtt107 collaborate to recruit partner proteins to chromatin. Our work thus illustrates how a master regulator uses two types of BRCT domains to recognize distinct genome factors and direct them to chromatin for constitutive genome protection.
Molecular Basis for Control of Diverse Genome Stability Factors by the Multi-BRCT Scaffold Rtt107.,Wan B, Wu J, Meng X, Lei M, Zhao X Mol Cell. 2019 Jul 25;75(2):238-251.e5. doi: 10.1016/j.molcel.2019.05.035. Epub, 2019 Jul 16. PMID:31348879[3]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Rouse J. Esc4p, a new target of Mec1p (ATR), promotes resumption of DNA synthesis after DNA damage. EMBO J. 2004 Mar 10;23(5):1188-97. Epub 2004 Feb 26. PMID:14988729 doi:http://dx.doi.org/10.1038/sj.emboj.7600129
- ↑ Roberts TM, Zaidi IW, Vaisica JA, Peter M, Brown GW. Regulation of rtt107 recruitment to stalled DNA replication forks by the cullin rtt101 and the rtt109 acetyltransferase. Mol Biol Cell. 2008 Jan;19(1):171-80. Epub 2007 Oct 31. PMID:17978089 doi:http://dx.doi.org/10.1091/mbc.E07-09-0961
- ↑ Wan B, Wu J, Meng X, Lei M, Zhao X. Molecular Basis for Control of Diverse Genome Stability Factors by the Multi-BRCT Scaffold Rtt107. Mol Cell. 2019 Jul 25;75(2):238-251.e5. doi: 10.1016/j.molcel.2019.05.035. Epub, 2019 Jul 16. PMID:31348879 doi:http://dx.doi.org/10.1016/j.molcel.2019.05.035
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