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| | ==Crystal structure of CARD-only protein in frog virus 3== | | ==Crystal structure of CARD-only protein in frog virus 3== |
| - | <StructureSection load='6j52' size='340' side='right' caption='[[6j52]], [[Resolution|resolution]] 2.50Å' scene=''> | + | <StructureSection load='6j52' size='340' side='right'caption='[[6j52]], [[Resolution|resolution]] 2.50Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[6j52]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Frg3v Frg3v]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6J52 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6J52 FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[6j52]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Frog_virus_3 Frog virus 3]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6J52 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6J52 FirstGlance]. <br> |
| - | </td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">orf64R, RUK13gorf64R, SSMEgorf64R ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10493 FRG3V])</td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.504Å</td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6j52 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6j52 OCA], [http://pdbe.org/6j52 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6j52 RCSB], [http://www.ebi.ac.uk/pdbsum/6j52 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6j52 ProSAT]</span></td></tr> | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6j52 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6j52 OCA], [https://pdbe.org/6j52 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6j52 RCSB], [https://www.ebi.ac.uk/pdbsum/6j52 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6j52 ProSAT]</span></td></tr> |
| | </table> | | </table> |
| | + | == Function == |
| | + | [https://www.uniprot.org/uniprot/W8SPG9_FRG3V W8SPG9_FRG3V] |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| | == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
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| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Frg3v]] | + | [[Category: Frog virus 3]] |
| - | [[Category: Kwon, S]] | + | [[Category: Large Structures]] |
| - | [[Category: Park, H H]] | + | [[Category: Kwon S]] |
| - | [[Category: Apoptosis]] | + | [[Category: Park HH]] |
| - | [[Category: Card-only protein]]
| + | |
| - | [[Category: Caspase recruitment domain]]
| + | |
| - | [[Category: Death domain superfamily]]
| + | |
| - | [[Category: Domain swapping]]
| + | |
| Structural highlights
Function
W8SPG9_FRG3V
Publication Abstract from PubMed
Caspase recruitment domain (CARD)-only proteins (COPs), regulate apoptosis, inflammation, and innate immunity. They inhibit the assembly of NOD-like receptor complexes such as the inflammasome and NODosome, which are molecular complexes critical for caspase-1 activation. COPs are known to interact with either caspase-1 CARD or RIP2 CARD via a CARD-CARD interaction, and inhibit caspase-1 activation or further downstream signaling. In addition to the human COPs, Pseudo-ICE, INCA, and ICEBERG, several viruses also contain viral COPs that help them escape the host immune system. To elucidate the molecular mechanism of host immunity inhibition by viral COPs, we solved the structure of a viral COP for the first time. Our structure showed that viral COP forms a structural transformation-mediated dimer, which is unique and has not been reported in any structural study of a CARD domain. Based on the current structure, and the previously solved structures of other death domain superfamily members, we propose that structural transformation-mediated dimerization might be a new strategy for dimer assembly in the death domain superfamily.
Structural transformation-mediated dimerization of caspase recruitment domain revealed by the crystal structure of CARD-only protein in frog virus 3.,Kim CM, Ha HJ, Kwon S, Jeong JH, Lee SH, Kim YG, Lee CS, Lee JH, Park HH J Struct Biol. 2019 Jan 6. pii: S1047-8477(19)30001-2. doi:, 10.1016/j.jsb.2018.12.006. PMID:30625366[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Kim CM, Ha HJ, Kwon S, Jeong JH, Lee SH, Kim YG, Lee CS, Lee JH, Park HH. Structural transformation-mediated dimerization of caspase recruitment domain revealed by the crystal structure of CARD-only protein in frog virus 3. J Struct Biol. 2019 Jan 6. pii: S1047-8477(19)30001-2. doi:, 10.1016/j.jsb.2018.12.006. PMID:30625366 doi:http://dx.doi.org/10.1016/j.jsb.2018.12.006
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