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| | <StructureSection load='6jjj' size='340' side='right'caption='[[6jjj]], [[Resolution|resolution]] 2.79Å' scene=''> | | <StructureSection load='6jjj' size='340' side='right'caption='[[6jjj]], [[Resolution|resolution]] 2.79Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[6jjj]] is a 6 chain structure with sequence from [http://en.wikipedia.org/wiki/Lk3_transgenic_mice Lk3 transgenic mice]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6JJJ OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6JJJ FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[6jjj]] is a 6 chain structure with sequence from [https://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6JJJ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6JJJ FirstGlance]. <br> |
| - | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.792Å</td></tr> |
| - | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">Clec4f, Clecsf13, Kclr ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10090 LK3 transgenic mice])</td></tr> | + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene></td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6jjj FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6jjj OCA], [http://pdbe.org/6jjj PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6jjj RCSB], [http://www.ebi.ac.uk/pdbsum/6jjj PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6jjj ProSAT]</span></td></tr> | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6jjj FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6jjj OCA], [https://pdbe.org/6jjj PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6jjj RCSB], [https://www.ebi.ac.uk/pdbsum/6jjj PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6jjj ProSAT]</span></td></tr> |
| | </table> | | </table> |
| | == Function == | | == Function == |
| - | [[http://www.uniprot.org/uniprot/CLC4F_MOUSE CLC4F_MOUSE]] Receptor with an affinity for galactose and fucose. Could be involved in endocytosis. | + | [https://www.uniprot.org/uniprot/CLC4F_MOUSE CLC4F_MOUSE] Receptor with an affinity for galactose and fucose. Could be involved in endocytosis. |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| | == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
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| | </StructureSection> | | </StructureSection> |
| | [[Category: Large Structures]] | | [[Category: Large Structures]] |
| - | [[Category: Lk3 transgenic mice]] | + | [[Category: Mus musculus]] |
| - | [[Category: Felix, J]] | + | [[Category: Felix J]] |
| - | [[Category: Ouyang, Z]] | + | [[Category: Ouyang Z]] |
| - | [[Category: Wen, Y]] | + | [[Category: Wen Y]] |
| - | [[Category: C-type lectin receptor]]
| + | |
| - | [[Category: Clec4f]]
| + | |
| - | [[Category: Kupffer cell receptor]]
| + | |
| - | [[Category: Sugar binding protein]]
| + | |
| Structural highlights
Function
CLC4F_MOUSE Receptor with an affinity for galactose and fucose. Could be involved in endocytosis.
Publication Abstract from PubMed
The C-type lectin receptor Clec4f has been identified as a specific surface marker for Kupffer cells, although its ortholog is absent in humans and its biological function remains elusive. Here, we report the crystal structure of a truncated mouse trimeric Clec4f. The orientation between the carbohydrate-recognition domain of Clec4f and its neck region differs from other C-type lectins, resulting in an observed distance of 45 A between the glycan-binding sites within the Clec4f trimer. Interestingly, the trimeric coiled-coil interface within its heptad neck region contains multiple polyglutamine interactions instead of the predominantly hydrophobic leucine zipper found in other C-type lectin receptors. The Clec4f trimeric structure displays unique features regarding its assembly and ligand recognition, shedding light on the evolution and diversity of the C-type lectin family.
Trimeric structure of the mouse Kupffer cell C-type lectin receptor Clec4f.,Ouyang Z, Felix J, Zhou J, Pei Y, Ma B, Hwang PM, Lemieux MJ, Gutsche I, Zheng F, Wen Y FEBS Lett. 2019 Aug 1. doi: 10.1002/1873-3468.13565. PMID:31369681[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Ouyang Z, Felix J, Zhou J, Pei Y, Ma B, Hwang PM, Lemieux MJ, Gutsche I, Zheng F, Wen Y. Trimeric structure of the mouse Kupffer cell C-type lectin receptor Clec4f. FEBS Lett. 2019 Aug 1. doi: 10.1002/1873-3468.13565. PMID:31369681 doi:http://dx.doi.org/10.1002/1873-3468.13565
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