1ofu
From Proteopedia
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'''CRYSTAL STRUCTURE OF SULA:FTSZ FROM PSEUDOMONAS AERUGINOSA''' | '''CRYSTAL STRUCTURE OF SULA:FTSZ FROM PSEUDOMONAS AERUGINOSA''' | ||
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[[Category: Lowe, J.]] | [[Category: Lowe, J.]] | ||
[[Category: Robinson, E J.H.]] | [[Category: Robinson, E J.H.]] | ||
| - | [[Category: | + | [[Category: Bacterial cell division inhibitor]] |
| - | [[Category: | + | [[Category: Ftsz]] |
| - | [[Category: | + | [[Category: Sula protein]] |
| - | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 03:47:44 2008'' | |
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | |
Revision as of 00:47, 3 May 2008
CRYSTAL STRUCTURE OF SULA:FTSZ FROM PSEUDOMONAS AERUGINOSA
Overview
SulA halts cell division in Escherichia coli by binding to the major component of the division machinery FtsZ. We have solved the crystal structure of SulA alone and in complex with FtsZ from Pseudomonas aeruginosa. SulA is expressed when the SOS response is induced. This is a mechanism to inhibit cell division and repair DNA in the event of DNA damage. FtsZ is a tubulin-like protein that forms polymers, with the active-site GTPase split across two monomers. One monomer provides the GTP-binding site and the other, through its T7 loop nucleotide hydrolysis. Our structures show that SulA is a dimer, and that SulA inhibits cell division neither by binding the nucleotide-binding site nor by inducing conformational changes in FtsZ. Instead, SulA binds the T7 loop surface of FtsZ, opposite the nucleotide-binding site, blocking polymer formation. These findings explain why GTP hydrolysis and polymer turnover are required for SulA inhibition.
About this Structure
1OFU is a Protein complex structure of sequences from Pseudomonas aeruginosa. Full crystallographic information is available from OCA.
Reference
Crystal structure of the SOS cell division inhibitor SulA and in complex with FtsZ., Cordell SC, Robinson EJ, Lowe J, Proc Natl Acad Sci U S A. 2003 Jun 24;100(13):7889-94. Epub 2003 Jun 13. PMID:12808143 Page seeded by OCA on Sat May 3 03:47:44 2008
