|
|
| Line 3: |
Line 3: |
| | <StructureSection load='6k5p' size='340' side='right'caption='[[6k5p]], [[Resolution|resolution]] 1.81Å' scene=''> | | <StructureSection load='6k5p' size='340' side='right'caption='[[6k5p]], [[Resolution|resolution]] 1.81Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[6k5p]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Culqu Culqu]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6K5P OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6K5P FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[6k5p]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Culex_quinquefasciatus Culex quinquefasciatus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6K5P OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6K5P FirstGlance]. <br> |
| - | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ACT:ACETATE+ION'>ACT</scene>, <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=CD:CADMIUM+ION'>CD</scene>, <scene name='pdbligand=CO:COBALT+(II)+ION'>CO</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.805Å</td></tr> |
| - | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">Cqm1 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=7176 CULQU])</td></tr>
| + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ACT:ACETATE+ION'>ACT</scene>, <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=CD:CADMIUM+ION'>CD</scene>, <scene name='pdbligand=CO:COBALT+(II)+ION'>CO</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6k5p FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6k5p OCA], [http://pdbe.org/6k5p PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6k5p RCSB], [http://www.ebi.ac.uk/pdbsum/6k5p PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6k5p ProSAT]</span></td></tr> | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6k5p FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6k5p OCA], [https://pdbe.org/6k5p PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6k5p RCSB], [https://www.ebi.ac.uk/pdbsum/6k5p PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6k5p ProSAT]</span></td></tr> |
| | </table> | | </table> |
| | + | == Function == |
| | + | [https://www.uniprot.org/uniprot/B0X223_CULQU B0X223_CULQU] |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| | == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
| Line 21: |
Line 23: |
| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Culqu]] | + | [[Category: Culex quinquefasciatus]] |
| | [[Category: Large Structures]] | | [[Category: Large Structures]] |
| - | [[Category: Kumar, V]] | + | [[Category: Kumar V]] |
| - | [[Category: Sharma, M]] | + | [[Category: Sharma M]] |
| - | [[Category: Amylomaltase]]
| + | |
| - | [[Category: Cqm1]]
| + | |
| - | [[Category: Gh13_17 subfamily]]
| + | |
| - | [[Category: Protein binding]]
| + | |
| - | [[Category: Receptor for binab toxin]]
| + | |
| Structural highlights
6k5p is a 4 chain structure with sequence from Culex quinquefasciatus. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
| | Method: | X-ray diffraction, Resolution 1.805Å |
| Ligands: | , , , , |
| Resources: | FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT |
Function
B0X223_CULQU
Publication Abstract from PubMed
Glycoside hydrolase 13 (GH13) family represents a large and diverse enzyme family. Cqm1, an amylomaltase of Culex mosquito, belongs to the GH13 family and subfamily 17 (GH13_17). The protein acts as the receptor for mosquito-larvicidal BinAB toxin that is used world-wide for control of the mosquito population. The protein was crystallized in the presence of a mixture of divalent metal ions. Cqm1 crystal structure was solved using the MRSAD method using Cd(II) anomalous at 1.9A wavelength and the structure was refined against 1.8A synchrotron data. One tightly bound Ca(II) ion in each of the monomer was observed and this site is suggested here to be unique to the GH13_17 family. Molecular dynamics simulations provide clues for the functional role of Ca(II) ion shown earlier to be essential for enzymatic activity. An optimized substrate (maltotriose) bound structure of the complex was constructed based on which 'retaining-type' mechanism can be predicted reliably. It reveals large conformational change in aromatic residues situated at active-site entrance. A Cd(II) ion was observed overlapping with the substrate-binding site. Kinetics data suggests non-competitive inhibition of Cqm1 by Cd(II). This is the first structure from the GH13_17 family and provides template for constructing reliable models for other members.
Crystal structure of BinAB toxin receptor (Cqm1) protein and molecular dynamics simulations reveal the role of unique Ca(II) ion.,Sharma M, Kumar V Int J Biol Macromol. 2019 Nov 1;140:1315-1325. doi:, 10.1016/j.ijbiomac.2019.08.126. Epub 2019 Aug 23. PMID:31449868[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Sharma M, Kumar V. Crystal structure of BinAB toxin receptor (Cqm1) protein and molecular dynamics simulations reveal the role of unique Ca(II) ion. Int J Biol Macromol. 2019 Nov 1;140:1315-1325. doi:, 10.1016/j.ijbiomac.2019.08.126. Epub 2019 Aug 23. PMID:31449868 doi:http://dx.doi.org/10.1016/j.ijbiomac.2019.08.126
|
