6kbc
From Proteopedia
(Difference between revisions)
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<StructureSection load='6kbc' size='340' side='right'caption='[[6kbc]], [[Resolution|resolution]] 1.99Å' scene=''> | <StructureSection load='6kbc' size='340' side='right'caption='[[6kbc]], [[Resolution|resolution]] 1.99Å' scene=''> | ||
== Structural highlights == | == Structural highlights == | ||
| - | <table><tr><td colspan='2'>[[6kbc]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/ | + | <table><tr><td colspan='2'>[[6kbc]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Chaetomium_globosum_CBS_148.51 Chaetomium globosum CBS 148.51]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6KBC OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6KBC FirstGlance]. <br> |
| - | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=WK1:(2S)-3-[(2S,4E)-4-[[(1R,2S,4aR,6S,8R,8aS)-2-[(E)-but-2-en-2-yl]-6,8-dimethyl-1,2,4a,5,6,7,8,8a-octahydronaphthalen-1-yl]-oxidanyl-methylidene]-3,5-bis(oxidanylidene)pyrrolidin-2-yl]-2-methyl-2-oxidanyl-propanoic+acid'>WK1</scene> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.99Å</td></tr> |
| - | + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=WK1:(2S)-3-[(2S,4E)-4-[[(1R,2S,4aR,6S,8R,8aS)-2-[(E)-but-2-en-2-yl]-6,8-dimethyl-1,2,4a,5,6,7,8,8a-octahydronaphthalen-1-yl]-oxidanyl-methylidene]-3,5-bis(oxidanylidene)pyrrolidin-2-yl]-2-methyl-2-oxidanyl-propanoic+acid'>WK1</scene></td></tr> | |
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6kbc FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6kbc OCA], [https://pdbe.org/6kbc PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6kbc RCSB], [https://www.ebi.ac.uk/pdbsum/6kbc PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6kbc ProSAT]</span></td></tr> | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6kbc FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6kbc OCA], [https://pdbe.org/6kbc PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6kbc RCSB], [https://www.ebi.ac.uk/pdbsum/6kbc PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6kbc ProSAT]</span></td></tr> | ||
</table> | </table> | ||
| + | == Function == | ||
| + | [https://www.uniprot.org/uniprot/CGHA_CHAGB CGHA_CHAGB] Diels-Alderase; part of the gene cluster that mediates the biosynthesis of the tetramic acid Sch210972, a potential anti-HIV fungal natural product that contains a decalin core (PubMed:26360642). The PKS module of cghG together with the enoylreductase cghC catalyze the formation of the polyketide unit which is then conjugated to 4-hydroxyl-4-methyl glutamate (HMG) by the condensation domain of the cghG NRPS module (PubMed:26360642). One unique structural feature of Sch210972 is the tetramic acid motif proposed to be derived from the non-proteinogenic amino acid HMG, by a Dieckmann-type condensation catalyzed by the reductase domain of cghG (PubMed:26360642). The aldolase cghB catalyzes the aldol condensation of 2 molecules of pyruvic acid to yield the intermediate 4-hydroxyl-4-methyl-2-oxoglutarate (HMOG), which can then be stereoselectively transaminated by an unidentified enzyme to form HMG (PubMed:26360642). The Diels-Alderase cghA then uses the Dieckmann product released by cghG as substrate and catalyzes the Diels-Alder cycloaddition to form the decalin ring of Sch210972 (PubMed:26360642). CghA also suppresses the nonenzymatic formation of the alternative stereoisomer (PubMed:26360642).<ref>PMID:26360642</ref> | ||
| + | == References == | ||
| + | <references/> | ||
__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
| - | [[Category: | + | [[Category: Chaetomium globosum CBS 148 51]] |
[[Category: Large Structures]] | [[Category: Large Structures]] | ||
| - | [[Category: Hara | + | [[Category: Hara K]] |
| - | [[Category: Hashimoto | + | [[Category: Hashimoto H]] |
| - | [[Category: Maeda | + | [[Category: Maeda N]] |
| - | [[Category: Sato | + | [[Category: Sato M]] |
| - | [[Category: Watanabe | + | [[Category: Watanabe K]] |
| - | + | ||
| - | + | ||
Current revision
Crystal structure of CghA with Sch210972
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