6kn9

From Proteopedia

(Difference between revisions)

Current revision

Crystal structure of human interleukin 18 receptor beta extracellular domain in complex with an antagonistic scFv

Structural highlights

6kn9 is a 6 chain structure with sequence from Homo sapiens. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:	X-ray diffraction, Resolution 3.302Å
Ligands:
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

I18RA_HUMAN

Publication Abstract from PubMed

The interleukin-18 subfamily belongs to the interleukin-1 family and plays an important role in modulating innate and adaptive immune responses. Dysregulation of IL-18 has been implicated in or correlated with numerous diseases, including inflammatory diseases, autoimmune disorders, and cancer. Thus, blockade of IL-18 signaling may offer therapeutic benefits in many pathological settings. Here, we report the development of synthetic human antibodies that target human IL-18Rbeta and block IL-18-mediated IFN-gamma secretion by inhibiting NF-kappaB and MAPK dependent pathways. The crystal structure of a potent antagonist antibody in complex with IL-18Rbeta revealed inhibition through an unexpected allosteric mechanism. Our findings offer a novel means for therapeutic intervention in the IL-18 pathway and may provide a new strategy for targeting cytokine receptors.

A Synthetic Human Antibody Antagonizes IL-18Rbeta Signaling Through an Allosteric Mechanism.,Liu S, Miersch S, Li P, Bai B, Liu C, Qin W, Su J, Huang H, Pan J, Sidhu SS, Wu D J Mol Biol. 2020 Jan 15. pii: S0022-2836(20)30042-5. doi:, 10.1016/j.jmb.2020.01.012. PMID:31954129^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Liu S, Miersch S, Li P, Bai B, Liu C, Qin W, Su J, Huang H, Pan J, Sidhu SS, Wu D. A Synthetic Human Antibody Antagonizes IL-18Rbeta Signaling Through an Allosteric Mechanism. J Mol Biol. 2020 Jan 15. pii: S0022-2836(20)30042-5. doi:, 10.1016/j.jmb.2020.01.012. PMID:31954129 doi:http://dx.doi.org/10.1016/j.jmb.2020.01.012

1 Structural highlights
2 Function
3 Publication Abstract from PubMed
4 See Also
5 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/6kn9"

Categories: Homo sapiens | Large Structures | Liu CC | Wu DH

@@ Line 3: / Line 3: @@
 <StructureSection load='6kn9' size='340' side='right'caption='[[6kn9]], [[Resolution|resolution]] 3.30&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[6kn9]] is a 6 chain structure with sequence from [http://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6KN9 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6KN9 FirstGlance]. <br>
+<table><tr><td colspan='2'>[[6kn9]] is a 6 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6KN9 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6KN9 FirstGlance]. <br>
-</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene></td></tr>
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 3.302&#8491;</td></tr>
-<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">IL18RAP, IL1R7 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])</td></tr>
+<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene></td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6kn9 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6kn9 OCA], [http://pdbe.org/6kn9 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6kn9 RCSB], [http://www.ebi.ac.uk/pdbsum/6kn9 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6kn9 ProSAT]</span></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6kn9 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6kn9 OCA], [https://pdbe.org/6kn9 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6kn9 RCSB], [https://www.ebi.ac.uk/pdbsum/6kn9 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6kn9 ProSAT]</span></td></tr>
 </table>
+== Function ==
+[https://www.uniprot.org/uniprot/I18RA_HUMAN I18RA_HUMAN]
 <div style="background-color:#fffaf0;">
 == Publication Abstract from PubMed ==
@@ Line 17: / Line 19: @@
 </div>
 <div class="pdbe-citations 6kn9" style="background-color:#fffaf0;"></div>
+==See Also==
+*[[Antibody 3D structures|Antibody 3D structures]]
+*[[Monoclonal Antibodies 3D structures|Monoclonal Antibodies 3D structures]]
 == References ==
 <references/>
 __TOC__
 </StructureSection>
-[[Category: Human]]
+[[Category: Homo sapiens]]
 [[Category: Large Structures]]
-[[Category: Liu, C C]]
+[[Category: Liu CC]]
-[[Category: Wu, D H]]
+[[Category: Wu DH]]
-[[Category: Antagonist]]
-[[Category: Antibody]]
-[[Category: Complex]]
-[[Category: Cytokine]]
-[[Category: Interleukin receptor]]

6kn9

From Proteopedia

Current revision

Crystal structure of human interleukin 18 receptor beta extracellular domain in complex with an antagonistic scFv

Structural highlights

Function

Publication Abstract from PubMed

See Also

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

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