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| | <StructureSection load='6kx9' size='340' side='right'caption='[[6kx9]], [[Resolution|resolution]] 2.90Å' scene=''> | | <StructureSection load='6kx9' size='340' side='right'caption='[[6kx9]], [[Resolution|resolution]] 2.90Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[6kx9]] is a 3 chain structure with sequence from [http://en.wikipedia.org/wiki/Chick Chick]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6KX9 OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=6KX9 FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[6kx9]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Gallus_gallus Gallus gallus] and [https://en.wikipedia.org/wiki/Synthetic_construct Synthetic construct]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6KX9 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6KX9 FirstGlance]. <br> |
| - | </td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">BF2 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9031 CHICK]), B2M ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9031 CHICK])</td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.902Å</td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=6kx9 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6kx9 OCA], [http://pdbe.org/6kx9 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6kx9 RCSB], [http://www.ebi.ac.uk/pdbsum/6kx9 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6kx9 ProSAT]</span></td></tr> | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6kx9 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6kx9 OCA], [https://pdbe.org/6kx9 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6kx9 RCSB], [https://www.ebi.ac.uk/pdbsum/6kx9 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6kx9 ProSAT]</span></td></tr> |
| | </table> | | </table> |
| | == Function == | | == Function == |
| - | [[http://www.uniprot.org/uniprot/B2MG_CHICK B2MG_CHICK]] Component of the class I major histocompatibility complex (MHC). Involved in the presentation of peptide antigens to the immune system. | + | [https://www.uniprot.org/uniprot/Q9GIP6_CHICK Q9GIP6_CHICK] |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| | == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
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| | ==See Also== | | ==See Also== |
| | *[[Beta-2 microglobulin 3D structures|Beta-2 microglobulin 3D structures]] | | *[[Beta-2 microglobulin 3D structures|Beta-2 microglobulin 3D structures]] |
| | + | *[[MHC 3D structures|MHC 3D structures]] |
| | + | *[[MHC I 3D structures|MHC I 3D structures]] |
| | == References == | | == References == |
| | <references/> | | <references/> |
| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Chick]] | + | [[Category: Gallus gallus]] |
| | [[Category: Large Structures]] | | [[Category: Large Structures]] |
| - | [[Category: Xiao, L]] | + | [[Category: Synthetic construct]] |
| - | [[Category: Zhang, L]] | + | [[Category: Xiao L]] |
| - | [[Category: 8-mer]] | + | [[Category: Zhang L]] |
| - | [[Category: Avian influenza virus]]
| + | |
| - | [[Category: Chicken]]
| + | |
| - | [[Category: Epitope]]
| + | |
| - | [[Category: H5n1]]
| + | |
| - | [[Category: Immune system]]
| + | |
| - | [[Category: Mhc class i]]
| + | |
| Structural highlights
Function
Q9GIP6_CHICK
Publication Abstract from PubMed
Lethal infections by strains of the highly-pathogenic avian influenza virus (HPAIV) H5N1 pose serious threats to both the poultry industry and public health worldwide. A lack of confirmed HPAIV epitopes recognized by cytotoxic T lymphocytes (CTLs) has hindered the utilization of CD8(+) T-cell-mediated immunity and has precluded the development of effectively diversified epitope-based vaccination approaches. In particular, an HPAIV H5N1 CTL-recognized epitope based on the peptide MHC-I-beta2m (pMHC-I) complex has not yet been designed. Here, screening a collection of selected peptides of several HPAIV strains against a specific pathogen-free pMHC-I (pBF2*1501), we identified a highly-conserved HPAIV H5N1 CTL epitope, named HPAIV-PA123-130 We determined the structure of the BF2*1501-PA123-130 complex at 2.1 A resolution to elucidate the molecular mechanisms of a preferential presentation of the highly-conserved PA123-130 epitope in the chicken B15 lineage. Conformational characteristics of the PA123-130 epitope with a protruding Tyr-7 residue indicated that this epitope has great potential to be recognized by specific TCRs. Moreover, significantly increased numbers of CD8(+) T cells specific for the HPAIV-PA123-130 epitope in peptide-immunized chickens indicated that a repertoire of CD8(+) T cells can specifically respond to this epitope. We anticipate that the identification and structural characterization of the PA123-130 epitope reported here could enable further studies of CTL immunity against HPAIV H5N1. Such studies may aid in the development of vaccine development strategies using well-conserved internal viral antigens in chickens.
Structures of the MHC-I molecule BF2*1501 disclose the preferred presentation of an H5N1 virus-derived epitope.,Li X, Zhang L, Liu Y, Ma L, Zhang N, Xia C J Biol Chem. 2020 Apr 17;295(16):5292-5306. doi: 10.1074/jbc.RA120.012713. Epub, 2020 Mar 9. PMID:32152225[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
See Also
References
- ↑ Li X, Zhang L, Liu Y, Ma L, Zhang N, Xia C. Structures of the MHC-I molecule BF2*1501 disclose the preferred presentation of an H5N1 virus-derived epitope. J Biol Chem. 2020 Apr 17;295(16):5292-5306. doi: 10.1074/jbc.RA120.012713. Epub, 2020 Mar 9. PMID:32152225 doi:http://dx.doi.org/10.1074/jbc.RA120.012713
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