6l3g

From Proteopedia

(Difference between revisions)

Current revision

Structural Basis for DNA Unwinding at Forked dsDNA by two coordinating Pif1 helicases

Structural highlights

6l3g is a 3 chain structure with sequence from Bacteroides sp. AF32-8BH and Homo sapiens. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:	X-ray diffraction, Resolution 3.3Å
Ligands:	, ,
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

A0A373G551_9BACE

Publication Abstract from PubMed

Pif1 plays multiple roles in maintaining genome stability and preferentially unwinds forked dsDNA, but the mechanism by which Pif1 unwinds forked dsDNA remains elusive. Here we report the structure of Bacteroides sp Pif1 (BaPif1) in complex with a symmetrical double forked dsDNA. Two interacting BaPif1 molecules are bound to each fork of the partially unwound dsDNA, and interact with the 5' arm and 3' ss/dsDNA respectively. Each of the two BaPif1 molecules is an active helicase and their interaction may regulate their helicase activities. The binding of BaPif1 to the 5' arm causes a sharp bend in the 5' ss/dsDNA junction, consequently breaking the first base-pair. BaPif1 bound to the 3' ss/dsDNA junction impacts duplex unwinding by stabilizing the unpaired first base-pair and engaging the second base-pair poised for breaking. Our results provide an unprecedented insight into how two BaPif1 coordinate with each other to unwind the forked dsDNA.

Structural basis for DNA unwinding at forked dsDNA by two coordinating Pif1 helicases.,Su N, Byrd AK, Bharath SR, Yang O, Jia Y, Tang X, Ha T, Raney KD, Song H Nat Commun. 2019 Nov 26;10(1):5375. doi: 10.1038/s41467-019-13414-9. PMID:31772234^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Su N, Byrd AK, Bharath SR, Yang O, Jia Y, Tang X, Ha T, Raney KD, Song H. Structural basis for DNA unwinding at forked dsDNA by two coordinating Pif1 helicases. Nat Commun. 2019 Nov 26;10(1):5375. doi: 10.1038/s41467-019-13414-9. PMID:31772234 doi:http://dx.doi.org/10.1038/s41467-019-13414-9

1 Structural highlights
2 Function
3 Publication Abstract from PubMed
4 See Also
5 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/6l3g"

@@ Line 3: / Line 3: @@
 <StructureSection load='6l3g' size='340' side='right'caption='[[6l3g]], [[Resolution|resolution]] 3.30&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[6l3g]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Bacteroides_sp._af32-8bh Bacteroides sp. af32-8bh]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6L3G OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6L3G FirstGlance]. <br>
+<table><tr><td colspan='2'>[[6l3g]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Bacteroides_sp._AF32-8BH Bacteroides sp. AF32-8BH] and [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6L3G OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6L3G FirstGlance]. <br>
-</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ADP:ADENOSINE-5-DIPHOSPHATE'>ADP</scene>, <scene name='pdbligand=ALF:TETRAFLUOROALUMINATE+ION'>ALF</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 3.3&#8491;</td></tr>
-<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">DWZ47_17845 ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=2302925 Bacteroides sp. AF32-8BH])</td></tr>
+<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ADP:ADENOSINE-5-DIPHOSPHATE'>ADP</scene>, <scene name='pdbligand=ALF:TETRAFLUOROALUMINATE+ION'>ALF</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
 <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6l3g FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6l3g OCA], [https://pdbe.org/6l3g PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6l3g RCSB], [https://www.ebi.ac.uk/pdbsum/6l3g PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6l3g ProSAT]</span></td></tr>
 </table>
+== Function ==
+[https://www.uniprot.org/uniprot/A0A373G551_9BACE A0A373G551_9BACE]
 <div style="background-color:#fffaf0;">
 == Publication Abstract from PubMed ==
@@ Line 24: / Line 26: @@
 __TOC__
 </StructureSection>
-[[Category: Bacteroides sp. af32-8bh]]
+[[Category: Bacteroides sp. AF32-8BH]]
+[[Category: Homo sapiens]]
 [[Category: Large Structures]]
-[[Category: Bharath, S R]]
+[[Category: Bharath SR]]
-[[Category: Song, H]]
+[[Category: Song H]]
-[[Category: Su, N]]
+[[Category: Su N]]
-[[Category: Atp dependent dna helicase]]
-[[Category: Dsdna unwinding]]
-[[Category: Hydrolase]]
-[[Category: Hydrolase-dna complex]]
-[[Category: Pif1]]

6l3g

From Proteopedia

Current revision

Structural Basis for DNA Unwinding at Forked dsDNA by two coordinating Pif1 helicases

Structural highlights

Function

Publication Abstract from PubMed

See Also

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

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