6l82
From Proteopedia
(Difference between revisions)
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| - | '''Unreleased structure''' | ||
| - | + | ==Crystal structure of Chaetomium GCP5 N-terminus and Mozart1== | |
| + | <StructureSection load='6l82' size='340' side='right'caption='[[6l82]], [[Resolution|resolution]] 2.24Å' scene=''> | ||
| + | == Structural highlights == | ||
| + | <table><tr><td colspan='2'>[[6l82]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Chaetomium_thermophilum_var._thermophilum_DSM_1495 Chaetomium thermophilum var. thermophilum DSM 1495]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6L82 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6L82 FirstGlance]. <br> | ||
| + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.2410362Å</td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6l82 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6l82 OCA], [https://pdbe.org/6l82 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6l82 RCSB], [https://www.ebi.ac.uk/pdbsum/6l82 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6l82 ProSAT]</span></td></tr> | ||
| + | </table> | ||
| + | == Function == | ||
| + | [https://www.uniprot.org/uniprot/G0SGA5_CHATD G0SGA5_CHATD] | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | How gamma-tubulin ring complex (gamma-TuRC), a master template for microtubule nucleation, is spatially and temporally regulated for the assembly of new microtubule arrays remains unclear. Here, we report that an evolutionarily conserved microprotein, Mozart1 (Mzt1), regulates subcellular targeting and microtubule formation activity of gamma-TuRC at different cell cycle stages. Crystal structures of protein complexes demonstrate that Mzt1 promiscuously interacts with the N-terminal domains of multiple gamma-tubulin complex protein subunits in gamma-TuRC via an intercalative binding mode. Genetic- and microscopy-based analyses show that promiscuous binding of Mzt1 in gamma-TuRC controls specific subcellular localization of gamma-TuRC to modulate microtubule nucleation and stabilization in fission yeast. Moreover, we find Mzt1-independent targeting of gamma-TuRC to be crucial for mitotic spindle assembly, demonstrating the cell-cycle-dependent regulation and function of gamma-TuRC. Our findings reveal a microprotein-mediated regulatory mechanism underlying microtubule cytoskeleton formation, whereby Mzt1 binding promiscuity confers localization specificity on the multi-protein complex gamma-TuRC. | ||
| - | + | Promiscuous Binding of Microprotein Mozart1 to gamma-Tubulin Complex Mediates Specific Subcellular Targeting to Control Microtubule Array Formation.,Huang TL, Wang HJ, Chang YC, Wang SW, Hsia KC Cell Rep. 2020 Jun 30;31(13):107836. doi: 10.1016/j.celrep.2020.107836. PMID:32610137<ref>PMID:32610137</ref> | |
| - | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |
| - | [[Category: | + | </div> |
| + | <div class="pdbe-citations 6l82" style="background-color:#fffaf0;"></div> | ||
| + | == References == | ||
| + | <references/> | ||
| + | __TOC__ | ||
| + | </StructureSection> | ||
| + | [[Category: Chaetomium thermophilum var. thermophilum DSM 1495]] | ||
| + | [[Category: Large Structures]] | ||
| + | [[Category: Hsia KC]] | ||
| + | [[Category: Huang TL]] | ||
| + | [[Category: Wang HJ]] | ||
| + | [[Category: Wang SW]] | ||
Current revision
Crystal structure of Chaetomium GCP5 N-terminus and Mozart1
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