6lf1

From Proteopedia

(Difference between revisions)

Current revision

SeviL, a GM1b/asialo-GM1 binding lectin

Structural highlights

6lf1 is a 2 chain structure with sequence from Mytilisepta virgata. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:	X-ray diffraction, Resolution 1.7Å
Ligands:
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

LECS1_MYTVI Galbeta1-3GalNAcbeta1-4Galbeta1-4Glc oligosaccharide-binding lectin. Binds strongly to the oligosaccharides of ganglioside GM1b and to a lesser extent its precursor asialo-GM1 (PubMed:31769916, PubMed:33328520). Binds weakly to asialo-GM2 oligosaccharide and to the glycan moiety of globo-series stage-specific embryonal antigen 4 (SSEA-4) hexaose (PubMed:31769916). Binds galactose, N-acetylgalactose and lactose. Does not bind GM1 (PubMed:33328520). Does not bind to Gal-beta1,3-GalNAc (Thomsen-Friedenreich antigen), the oligosaccharide of GM1a ganglioside or SSEA-4 tetraose. Does not bind to N-glycans, O-glycans or glycosaminoglycans of glycoproteins. Does not bind Lewis glycans, derivatives of lactose or N-acetyllactosamine or blood group (ABH-type) oligosaccharides (PubMed:31769916). Does not bind glucose (PubMed:33328520). Has hemagglutination activity towards rabbit erythrocytes (PubMed:31769916, PubMed:33328520). Displays cytotoxic effects against various cultured cell lines including human breast (MCF-7), cervical (HeLa) and colon cancer (Caco2) cell lines, as well as dog kidney (MDCK) cell line that express asialo-GM1 oligosaccharide at their cell surface. Shows dose- and time-dependent activation of MKK3/6, ERK1/2 and p38 MAPK, as well as caspase-3/9 in HeLa cervical cancer cells. No cytotoxic effect on BT474 human breast cancer cell line. May be involved in recognition of glycans found on parasitic or symbiotic microorganisms (PubMed:31769916).^[1] ^[2]

Publication Abstract from PubMed

SeviL is a recently isolated lectin found to bind to the linear saccharides of the ganglioside GM1b (Neu5Ac[Formula: see text](2-3)Gal[Formula: see text](1-3)GalNAc[Formula: see text](1-4)Gal[Formula: see text](1-4)Glc) and its precursor, asialo-GM1 (Gal[Formula: see text](1-3)GalNAc[Formula: see text](1-4)Gal[Formula: see text](1-4)Glc). The crystal structures of recombinant SeviL have been determined in the presence and absence of ligand. The protein belongs to the [Formula: see text]-trefoil family, but shows only weak sequence similarity to known structures. SeviL forms a dimer in solution, with one binding site per subunit, close to the subunit interface. Molecular details of glycan recognition by SeviL in solution were analysed by ligand- and protein-based NMR techniques as well as ligand binding assays. SeviL shows no interaction with GM1 due to steric hindrance with the sialic acid branch that is absent from GM1b. This unusual specificity makes SeviL of great interest for the detection and control of certain cancer cells, and cells of the immune system, that display asialo-GM1.

The structure of SeviL, a GM1b/asialo-GM1 binding R-type lectin from the mussel Mytilisepta virgata.,Kamata K, Mizutani K, Takahashi K, Marchetti R, Silipo A, Addy C, Park SY, Fujii Y, Fujita H, Konuma T, Ikegami T, Ozeki Y, Tame JRH Sci Rep. 2020 Dec 16;10(1):22102. doi: 10.1038/s41598-020-78926-7. PMID:33328520^[3]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Fujii Y, Gerdol M, Kawsar SMA, Hasan I, Spazzali F, Yoshida T, Ogawa Y, Rajia S, Kamata K, Koide Y, Sugawara S, Hosono M, Tame JRH, Fujita H, Pallavicini A, Ozeki Y. A GM1b/asialo-GM1 oligosaccharide-binding R-type lectin from purplish bifurcate mussels Mytilisepta virgata and its effect on MAP kinases. FEBS J. 2020 Jun;287(12):2612-2630. PMID:31769916 doi:10.1111/febs.15154
↑ Kamata K, Mizutani K, Takahashi K, Marchetti R, Silipo A, Addy C, Park SY, Fujii Y, Fujita H, Konuma T, Ikegami T, Ozeki Y, Tame JRH. The structure of SeviL, a GM1b/asialo-GM1 binding R-type lectin from the mussel Mytilisepta virgata. Sci Rep. 2020 Dec 16;10(1):22102. doi: 10.1038/s41598-020-78926-7. PMID:33328520 doi:http://dx.doi.org/10.1038/s41598-020-78926-7
↑ Kamata K, Mizutani K, Takahashi K, Marchetti R, Silipo A, Addy C, Park SY, Fujii Y, Fujita H, Konuma T, Ikegami T, Ozeki Y, Tame JRH. The structure of SeviL, a GM1b/asialo-GM1 binding R-type lectin from the mussel Mytilisepta virgata. Sci Rep. 2020 Dec 16;10(1):22102. doi: 10.1038/s41598-020-78926-7. PMID:33328520 doi:http://dx.doi.org/10.1038/s41598-020-78926-7

1 Structural highlights
2 Function
3 Publication Abstract from PubMed
4 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/6lf1"

@@ Line 3: / Line 3: @@
 <StructureSection load='6lf1' size='340' side='right'caption='[[6lf1]], [[Resolution|resolution]] 1.70&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[6lf1]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6LF1 OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=6LF1 FirstGlance]. <br>
+<table><tr><td colspan='2'>[[6lf1]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Mytilisepta_virgata Mytilisepta virgata]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6LF1 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6LF1 FirstGlance]. <br>
-</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene></td></tr>
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.7&#8491;</td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=6lf1 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6lf1 OCA], [http://pdbe.org/6lf1 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6lf1 RCSB], [http://www.ebi.ac.uk/pdbsum/6lf1 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6lf1 ProSAT]</span></td></tr>
+<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6lf1 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6lf1 OCA], [https://pdbe.org/6lf1 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6lf1 RCSB], [https://www.ebi.ac.uk/pdbsum/6lf1 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6lf1 ProSAT]</span></td></tr>
 </table>
+== Function ==
+[https://www.uniprot.org/uniprot/LECS1_MYTVI LECS1_MYTVI] Galbeta1-3GalNAcbeta1-4Galbeta1-4Glc oligosaccharide-binding lectin. Binds strongly to the oligosaccharides of ganglioside GM1b and to a lesser extent its precursor asialo-GM1 (PubMed:31769916, PubMed:33328520). Binds weakly to asialo-GM2 oligosaccharide and to the glycan moiety of globo-series stage-specific embryonal antigen 4 (SSEA-4) hexaose (PubMed:31769916). Binds galactose, N-acetylgalactose and lactose. Does not bind GM1 (PubMed:33328520). Does not bind to Gal-beta1,3-GalNAc (Thomsen-Friedenreich antigen), the oligosaccharide of GM1a ganglioside or SSEA-4 tetraose. Does not bind to N-glycans, O-glycans or glycosaminoglycans of glycoproteins. Does not bind Lewis glycans, derivatives of lactose or N-acetyllactosamine or blood group (ABH-type) oligosaccharides (PubMed:31769916). Does not bind glucose (PubMed:33328520). Has hemagglutination activity towards rabbit erythrocytes (PubMed:31769916, PubMed:33328520). Displays cytotoxic effects against various cultured cell lines including human breast (MCF-7), cervical (HeLa) and colon cancer (Caco2) cell lines, as well as dog kidney (MDCK) cell line that express asialo-GM1 oligosaccharide at their cell surface. Shows dose- and time-dependent activation of MKK3/6, ERK1/2 and p38 MAPK, as well as caspase-3/9 in HeLa cervical cancer cells. No cytotoxic effect on BT474 human breast cancer cell line. May be involved in recognition of glycans found on parasitic or symbiotic microorganisms (PubMed:31769916).<ref>PMID:31769916</ref> <ref>PMID:33328520</ref>
 <div style="background-color:#fffaf0;">
 == Publication Abstract from PubMed ==
@@ Line 21: / Line 24: @@
 </StructureSection>
 [[Category: Large Structures]]
-[[Category: Kamata, K]]
+[[Category: Mytilisepta virgata]]
-[[Category: Ozeki, Y]]
+[[Category: Kamata K]]
-[[Category: Park, S Y]]
+[[Category: Ozeki Y]]
-[[Category: Tame, J R.H]]
+[[Category: Park S-Y]]
-[[Category: Anti-cancer]]
+[[Category: Tame JRH]]
-[[Category: Apoptosis]]
-[[Category: Mussel]]
-[[Category: Sugar binding protein]]
-[[Category: Trefoil]]

6lf1

From Proteopedia

Current revision

SeviL, a GM1b/asialo-GM1 binding lectin

Structural highlights

Function

Publication Abstract from PubMed

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

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