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| | <StructureSection load='6lg2' size='340' side='right'caption='[[6lg2]], [[Resolution|resolution]] 1.60Å' scene=''> | | <StructureSection load='6lg2' size='340' side='right'caption='[[6lg2]], [[Resolution|resolution]] 1.60Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[6lg2]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Corgl Corgl]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6LG2 OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=6LG2 FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[6lg2]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Corynebacterium_glutamicum_ATCC_13032 Corynebacterium glutamicum ATCC 13032]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6LG2 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6LG2 FirstGlance]. <br> |
| - | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=VNL:4-HYDROXY-3-METHOXYBENZOATE'>VNL</scene></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.6Å</td></tr> |
| - | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">Cgl2382 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=196627 CORGL])</td></tr> | + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=VNL:4-HYDROXY-3-METHOXYBENZOATE'>VNL</scene></td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=6lg2 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6lg2 OCA], [http://pdbe.org/6lg2 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6lg2 RCSB], [http://www.ebi.ac.uk/pdbsum/6lg2 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6lg2 ProSAT]</span></td></tr> | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6lg2 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6lg2 OCA], [https://pdbe.org/6lg2 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6lg2 RCSB], [https://www.ebi.ac.uk/pdbsum/6lg2 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6lg2 ProSAT]</span></td></tr> |
| | </table> | | </table> |
| | + | == Function == |
| | + | [https://www.uniprot.org/uniprot/Q8NN31_CORGL Q8NN31_CORGL] |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| | == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
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| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Corgl]] | + | [[Category: Corynebacterium glutamicum ATCC 13032]] |
| | [[Category: Large Structures]] | | [[Category: Large Structures]] |
| - | [[Category: Bharath, S R]] | + | [[Category: Bharath SR]] |
| - | [[Category: He, Y]] | + | [[Category: He Y]] |
| - | [[Category: Song, H]] | + | [[Category: Song H]] |
| - | [[Category: Transcription]]
| + | |
| - | [[Category: Vanr transcription factor vanillate]]
| + | |
| Structural highlights
Function
Q8NN31_CORGL
Publication Abstract from PubMed
Hydroxytyrosol is an antioxidant free radical scavenger that is biosynthesized from tyrosine. In metabolic engineering efforts, the use of the mouse tyrosine hydroxylase limits its production. Here, we design an efficient whole-cell catalyst of hydroxytyrosol in Escherichia coli by de-bottlenecking two rate-limiting enzymatic steps. First, we replace the mouse tyrosine hydroxylase by an engineered two-component flavin-dependent monooxygenase HpaBC of E. coli through structure-guided modeling and directed evolution. Next, we elucidate the structure of the Corynebacterium glutamicum VanR regulatory protein complexed with its inducer vanillic acid. By switching its induction specificity from vanillic acid to hydroxytyrosol, VanR is engineered into a hydroxytyrosol biosensor. Then, with this biosensor, we use in vivo-directed evolution to optimize the activity of tyramine oxidase (TYO), the second rate-limiting enzyme in hydroxytyrosol biosynthesis. The final strain reaches a 95% conversion rate of tyrosine. This study demonstrates the effectiveness of sequentially de-bottlenecking rate-limiting steps for whole-cell catalyst development.
Developing a highly efficient hydroxytyrosol whole-cell catalyst by de-bottlenecking rate-limiting steps.,Yao J, He Y, Su N, Bharath SR, Tao Y, Jin JM, Chen W, Song H, Tang SY Nat Commun. 2020 Mar 23;11(1):1515. doi: 10.1038/s41467-020-14918-5. PMID:32251291[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Yao J, He Y, Su N, Bharath SR, Tao Y, Jin JM, Chen W, Song H, Tang SY. Developing a highly efficient hydroxytyrosol whole-cell catalyst by de-bottlenecking rate-limiting steps. Nat Commun. 2020 Mar 23;11(1):1515. doi: 10.1038/s41467-020-14918-5. PMID:32251291 doi:http://dx.doi.org/10.1038/s41467-020-14918-5
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