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| | <StructureSection load='5nx0' size='340' side='right'caption='[[5nx0]], [[Resolution|resolution]] 1.80Å' scene=''> | | <StructureSection load='5nx0' size='340' side='right'caption='[[5nx0]], [[Resolution|resolution]] 1.80Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[5nx0]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Bpt4 Bpt4]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5NX0 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5NX0 FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[5nx0]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_virus_T4 Escherichia virus T4]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5NX0 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5NX0 FirstGlance]. <br> |
| - | </td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">e, T4Tp126 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10665 BPT4])</td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.803Å</td></tr> |
| - | <tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Lysozyme Lysozyme], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.17 3.2.1.17] </span></td></tr>
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5nx0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5nx0 OCA], [https://pdbe.org/5nx0 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5nx0 RCSB], [https://www.ebi.ac.uk/pdbsum/5nx0 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5nx0 ProSAT]</span></td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5nx0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5nx0 OCA], [http://pdbe.org/5nx0 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5nx0 RCSB], [http://www.ebi.ac.uk/pdbsum/5nx0 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5nx0 ProSAT]</span></td></tr> | + | |
| | </table> | | </table> |
| | + | == Function == |
| | + | [https://www.uniprot.org/uniprot/D9IEF7_BPT4 D9IEF7_BPT4] |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| | == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
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| | </div> | | </div> |
| | <div class="pdbe-citations 5nx0" style="background-color:#fffaf0;"></div> | | <div class="pdbe-citations 5nx0" style="background-color:#fffaf0;"></div> |
| | + | |
| | + | ==See Also== |
| | + | *[[Lysin 3D structures|Lysin 3D structures]] |
| | == References == | | == References == |
| | <references/> | | <references/> |
| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Bpt4]] | + | [[Category: Escherichia virus T4]] |
| | [[Category: Large Structures]] | | [[Category: Large Structures]] |
| - | [[Category: Lysozyme]]
| + | [[Category: Consentius P]] |
| - | [[Category: Consentius, P]] | + | [[Category: Gohlke U]] |
| - | [[Category: Gohlke, U]] | + | [[Category: Heinemann U]] |
| - | [[Category: Heinemann, U]] | + | [[Category: Kaupp M]] |
| - | [[Category: Kaupp, M]] | + | [[Category: Loll B]] |
| - | [[Category: Loll, B]] | + | [[Category: Mueller R]] |
| - | [[Category: Mueller, R]] | + | [[Category: Risse T]] |
| - | [[Category: Risse, T]] | + | [[Category: Wahl MC]] |
| - | [[Category: Wahl, M C]] | + | |
| - | [[Category: Electron paramagnetic resonance]]
| + | |
| - | [[Category: Epr]]
| + | |
| - | [[Category: Hydrolase]]
| + | |
| - | [[Category: Nitroxide]]
| + | |
| - | [[Category: Spin label]]
| + | |
| - | [[Category: T4 lysozyme]]
| + | |
| Structural highlights
Function
D9IEF7_BPT4
Publication Abstract from PubMed
Electron paramagnetic resonance (EPR) spectroscopy in combination with site-directed spin labeling is used to investigate the structure and dynamics of conformationally constrained spin labels in T4 lysozyme single crystals. Within a single crystal, the oriented ensemble of spin bearing moieties results in a strong angle dependence of the EPR spectra. A quantitative description of the EPR spectra requires the determination of the unit cell orientation with respect to the sample tube and the orientation of the spin bearing moieties within the crystal lattice. Angle dependent EPR spectra were analyzed by line shape simulations using the stochastic Liouville equation approach developed by Freed and co-workers and an effective Hamiltonian approach. The gain in spectral information obtained from the EPR spectra of single crystalline samples taken at different frequencies, namely the X-band and Q-band, allows us to discriminate between motional models describing the spectra of isotropic solutions similarly well. In addition, it is shown that the angle dependent single crystal spectra allow us to identify two spin label rotamers with very similar side chain dynamics. These results demonstrate the utility of single crystal EPR spectroscopy in combination with spectral line shape simulation techniques to extract valuable dynamic information not readily available from the analysis of isotropic systems. In addition, it will be shown that the loss of electron density in high resolution diffraction experiments at room temperature does not allow us to conclude that there is significant structural disorder in the system.
Combining EPR spectroscopy and X-ray crystallography to elucidate the structure and dynamics of conformationally constrained spin labels in T4 lysozyme single crystals.,Consentius P, Gohlke U, Loll B, Alings C, Heinemann U, Wahl MC, Risse T Phys Chem Chem Phys. 2017 Aug 9;19(31):20723-20734. doi: 10.1039/c7cp03144k. PMID:28740983[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
See Also
References
- ↑ Consentius P, Gohlke U, Loll B, Alings C, Heinemann U, Wahl MC, Risse T. Combining EPR spectroscopy and X-ray crystallography to elucidate the structure and dynamics of conformationally constrained spin labels in T4 lysozyme single crystals. Phys Chem Chem Phys. 2017 Aug 9;19(31):20723-20734. doi: 10.1039/c7cp03144k. PMID:28740983 doi:http://dx.doi.org/10.1039/c7cp03144k
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