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| | <StructureSection load='5nz8' size='340' side='right'caption='[[5nz8]], [[Resolution|resolution]] 3.00Å' scene=''> | | <StructureSection load='5nz8' size='340' side='right'caption='[[5nz8]], [[Resolution|resolution]] 3.00Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[5nz8]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/"ruminiclostridium_thermocellum"_(viljoen_et_al._1926)_yutin_and_galperin_2013 "ruminiclostridium thermocellum" (viljoen et al. 1926) yutin and galperin 2013]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5NZ8 OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=5NZ8 FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[5nz8]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Acetivibrio_thermocellus Acetivibrio thermocellus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5NZ8 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5NZ8 FirstGlance]. <br> |
| - | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=BGC:BETA-D-GLUCOSE'>BGC</scene>, <scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 3Å</td></tr> |
| - | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">cdp-ym4 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1515 "Ruminiclostridium thermocellum" (Viljoen et al. 1926) Yutin and Galperin 2013])</td></tr> | + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=BGC:BETA-D-GLUCOSE'>BGC</scene>, <scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene>, <scene name='pdbligand=PRD_900011:beta-cellotetraose'>PRD_900011</scene></td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=5nz8 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5nz8 OCA], [http://pdbe.org/5nz8 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5nz8 RCSB], [http://www.ebi.ac.uk/pdbsum/5nz8 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5nz8 ProSAT]</span></td></tr> | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5nz8 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5nz8 OCA], [https://pdbe.org/5nz8 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5nz8 RCSB], [https://www.ebi.ac.uk/pdbsum/5nz8 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5nz8 ProSAT]</span></td></tr> |
| | </table> | | </table> |
| | + | == Function == |
| | + | [https://www.uniprot.org/uniprot/Q93HT8_ACETH Q93HT8_ACETH] |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| | == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
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| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| | + | [[Category: Acetivibrio thermocellus]] |
| | [[Category: Large Structures]] | | [[Category: Large Structures]] |
| - | [[Category: Field, R A]] | + | [[Category: Field RA]] |
| - | [[Category: Lawson, D M]] | + | [[Category: Lawson DM]] |
| - | [[Category: Neill, E C.O]] | + | [[Category: Nepogodiev SA]] |
| - | [[Category: Nepogodiev, S A]] | + | [[Category: O'Neill EC]] |
| - | [[Category: Pergolizzi, G]] | + | [[Category: Pergolizzi G]] |
| - | [[Category: Stevenson, C E.M]] | + | [[Category: Stevenson CEM]] |
| - | [[Category: Cellodextrin phosphorylase]]
| + | |
| - | [[Category: Cellulose synthesis]]
| + | |
| - | [[Category: Glycoside hydrolase family 94]]
| + | |
| - | [[Category: Hydrolase]]
| + | |
| Structural highlights
Function
Q93HT8_ACETH
Publication Abstract from PubMed
The GH94 glycoside hydrolase cellodextrin phosphorylase (CDP, EC 2.4.1.49) produces cellodextrin oligomers from short beta-1-->4-glucans and alpha-D-glucose 1-phosphate. Compared to cellobiose phosphorylase (CBP), which produces cellobiose from glucose and alpha-D-glucose 1-phosphate, CDP is biochemically less well characterised. Herein, we investigate the donor and acceptor substrate specificity of recombinant CDP from Ruminiclostridium thermocellum and we isolate and characterise a glucosamine addition product to the cellobiose acceptor with the non-natural donor alpha-D-glucosamine 1-phosphate. In addition, we report the first X-ray crystal structure of CDP, along with comparison to the available structures from CBPs and other closely related enzymes, which contributes to understanding of the key structural features necessary to discriminate between monosaccharide (CBP) and oligosaccharide (CDP) acceptor substrates.
Cellodextrin phosphorylase from Ruminiclostridium thermocellum: X-ray crystal structure and substrate specificity analysis.,O'Neill EC, Pergolizzi G, Stevenson CEM, Lawson DM, Nepogodiev SA, Field RA Carbohydr Res. 2017 Jul 21. pii: S0008-6215(17)30359-2. doi:, 10.1016/j.carres.2017.07.005. PMID:28760417[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ O'Neill EC, Pergolizzi G, Stevenson CEM, Lawson DM, Nepogodiev SA, Field RA. Cellodextrin phosphorylase from Ruminiclostridium thermocellum: X-ray crystal structure and substrate specificity analysis. Carbohydr Res. 2017 Jul 21. pii: S0008-6215(17)30359-2. doi:, 10.1016/j.carres.2017.07.005. PMID:28760417 doi:http://dx.doi.org/10.1016/j.carres.2017.07.005
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