1pq2
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(New page: 200px<br /> <applet load="1pq2" size="450" color="white" frame="true" align="right" spinBox="true" caption="1pq2, resolution 2.7Å" /> '''Crystal Structure of...)
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Revision as of 16:39, 12 November 2007
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Crystal Structure of Human Drug Metabolizing Cytochrome P450 2C8
Overview
A 2.7-Angstrom molecular structure of human microsomal cytochrome P450 2C8, (CYP2C8) was determined by x-ray crystallography. The membrane protein was, modified for crystallization by replacement of the hydrophobic N-terminal, transmembrane domain with a short hydrophilic sequence before residue 28., The structure of the native sequence is complete from residue 28 to the, beginning of a C-terminal histidine tag used for purification. CYP2C8 is, one of the principal hepatic drug-metabolizing enzymes that oxidizes, therapeutic drugs such as taxol and cerivastatin and endobiotics such as, retinoic acid and arachidonic acid. Consistent with the relatively large, size of its preferred substrates, the active site volume is twice that, observed for the structure of CYP2C5. The extended active site cavity is, bounded by the beta1 sheet and helix F' that have not previously been, implicated in substrate recognition by mammalian P450s. CYP2C8, crystallized as a symmetric dimer formed by the interaction of helices F, F', G', and G. Two molecules of palmitic acid are bound in the dimer, interface. The dimer is observed in solution, and mass spectrometry, confirmed the association of palmitic acid with the enzyme. This novel, finding identifies a peripheral binding site in P450s that may contribute, to drug-drug interactions in P450 metabolism.
About this Structure
1PQ2 is a Single protein structure of sequence from Homo sapiens with PO4, HEM and PLM as ligands. Active as Unspecific monooxygenase, with EC number 1.14.14.1 Full crystallographic information is available from OCA.
Reference
Structure of human microsomal cytochrome P450 2C8. Evidence for a peripheral fatty acid binding site., Schoch GA, Yano JK, Wester MR, Griffin KJ, Stout CD, Johnson EF, J Biol Chem. 2004 Mar 5;279(10):9497-503. Epub 2003 Dec 15. PMID:14676196
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