8t8i
From Proteopedia
(Difference between revisions)
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| - | '''Unreleased structure''' | ||
| - | + | ==Structure of VHH-Fab complex with engineered Elbow FNQIKG, Crystal Kappa and SER substitutions== | |
| + | <StructureSection load='8t8i' size='340' side='right'caption='[[8t8i]], [[Resolution|resolution]] 2.52Å' scene=''> | ||
| + | == Structural highlights == | ||
| + | <table><tr><td colspan='2'>[[8t8i]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=8T8I OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=8T8I FirstGlance]. <br> | ||
| + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.52Å</td></tr> | ||
| + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=PEG:DI(HYDROXYETHYL)ETHER'>PEG</scene>, <scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene>, <scene name='pdbligand=SIN:SUCCINIC+ACID'>SIN</scene></td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=8t8i FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=8t8i OCA], [https://pdbe.org/8t8i PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=8t8i RCSB], [https://www.ebi.ac.uk/pdbsum/8t8i PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=8t8i ProSAT]</span></td></tr> | ||
| + | </table> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | The atomic-resolution structural information that X-ray crystallography can provide on the binding interface between a Fab and its cognate antigen is highly valuable for understanding the mechanism of interaction. However, many Fab:antigen complexes are recalcitrant to crystallization, making the endeavor a considerable effort with no guarantee of success. Consequently, there have been significant steps taken to increase the likelihood of Fab:antigen complex crystallization by altering the Fab framework. In this investigation, we applied the surface entropy reduction strategy coupled with phage-display technology to identify a set of surface substitutions that improve the propensity of a human Fab framework to crystallize. In addition, we showed that combining these surface substitutions with previously reported Crystal Kappa and elbow substitutions results in an extraordinary improvement in Fab and Fab:antigen complex crystallisability, revealing a strong synergistic relationship between these sets of substitutions. Through comprehensive Fab and Fab:antigen complex crystallization screenings followed by structure determination and analysis, we defined the roles that each of these substitutions play in facilitating crystallization and how they complement each other in the process. This article is protected by copyright. All rights reserved. | ||
| - | + | Engineered Antigen-Binding Fragments for Enhanced Crystallization of Antibody:Antigen Complexes.,Bruce HA, Singer AU, Filippova EV, Blazer LL, Adams JJ, Enderle L, Ben-David M, Radley EH, Mao DYL, Pau V, Orlicky S, Sicheri F, Kourinov I, Atwell S, Kossiakoff AA, Sidhu SS Protein Sci. 2023 Nov 9:e4824. doi: 10.1002/pro.4824. PMID:37945533<ref>PMID:37945533</ref> | |
| - | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |
| - | [[Category: | + | </div> |
| + | <div class="pdbe-citations 8t8i" style="background-color:#fffaf0;"></div> | ||
| + | == References == | ||
| + | <references/> | ||
| + | __TOC__ | ||
| + | </StructureSection> | ||
| + | [[Category: Homo sapiens]] | ||
| + | [[Category: Large Structures]] | ||
| + | [[Category: Filippova EV]] | ||
| + | [[Category: Kossiakoff AA]] | ||
| + | [[Category: Thompson I]] | ||
Revision as of 14:10, 29 November 2023
Structure of VHH-Fab complex with engineered Elbow FNQIKG, Crystal Kappa and SER substitutions
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