6lqk
From Proteopedia
(Difference between revisions)
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==Crystal structure of honeybee RyR NTD== | ==Crystal structure of honeybee RyR NTD== | ||
- | <StructureSection load='6lqk' size='340' side='right'caption='[[6lqk]]' scene=''> | + | <StructureSection load='6lqk' size='340' side='right'caption='[[6lqk]], [[Resolution|resolution]] 2.50Å' scene=''> |
== Structural highlights == | == Structural highlights == | ||
- | <table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6LQK OCA]. For a <b>guided tour on the structure components</b> use [ | + | <table><tr><td colspan='2'>[[6lqk]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Apis_mellifera Apis mellifera]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6LQK OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6LQK FirstGlance]. <br> |
- | </td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[ | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.499Å</td></tr> |
+ | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr> | ||
+ | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6lqk FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6lqk OCA], [https://pdbe.org/6lqk PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6lqk RCSB], [https://www.ebi.ac.uk/pdbsum/6lqk PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6lqk ProSAT]</span></td></tr> | ||
</table> | </table> | ||
+ | <div style="background-color:#fffaf0;"> | ||
+ | == Publication Abstract from PubMed == | ||
+ | Ryanodine receptors (RyRs) are the molecular target of diamides, a new chemical class of insecticides. Diamide insecticides are used to control lepidopteran pests and were considered relatively safe for mammals and non-targeted beneficial insects, including honey bees. However, recent studies showed that exposure to diamides could cause long-lasting locomotor deficits of bees. Here we report the crystal structure of RyR N-terminal domain A (NTD-A) from the honeybee, Apis mellifera, at 2.5 A resolution. It shows a similar overall fold as the RyR NTD-A from mammals and the diamondback moth (DBM), Plutella xylostella, and still several loops located at the inter-domain interfaces show insect-specific or bee-specific structural features. A potential insecticide-binding pocket formed by loop9 and loop13 is conserved in lepidopteran but different in both mammals and bees, making it a good candidate targeting site for the development of pest-selective insecticides. Furthermore, a conserved intra-domain disulfide bond was observed in both DBM and bee RyR NTD-A crystal structures, which explains their higher thermal stability compared to mammalian RyR NTD-A. This work provides a basis for the development of novel insecticides with better selectivity between pests and bees by targeting a distinct site on pest RyRs, which would be a promising strategy to overcome the current toxicity problem. | ||
+ | |||
+ | Crystal structure of the N-terminal domain of ryanodine receptor from the honeybee, Apis mellifera.,Zhou Y, Wang W, Salauddin NM, Lin L, You M, You S, Yuchi Z Insect Biochem Mol Biol. 2020 Oct;125:103454. doi: 10.1016/j.ibmb.2020.103454., Epub 2020 Aug 8. PMID:32781205<ref>PMID:32781205</ref> | ||
+ | |||
+ | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | ||
+ | </div> | ||
+ | <div class="pdbe-citations 6lqk" style="background-color:#fffaf0;"></div> | ||
+ | |||
+ | ==See Also== | ||
+ | *[[Ryanodine receptor 3D structures|Ryanodine receptor 3D structures]] | ||
+ | == References == | ||
+ | <references/> | ||
__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
+ | [[Category: Apis mellifera]] | ||
[[Category: Large Structures]] | [[Category: Large Structures]] | ||
[[Category: Lin L]] | [[Category: Lin L]] | ||
[[Category: Yuchi Z]] | [[Category: Yuchi Z]] | ||
[[Category: Zhou Y]] | [[Category: Zhou Y]] |
Revision as of 14:45, 29 November 2023
Crystal structure of honeybee RyR NTD
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