6lqk

From Proteopedia

(Difference between revisions)
Jump to: navigation, search
Line 1: Line 1:
==Crystal structure of honeybee RyR NTD==
==Crystal structure of honeybee RyR NTD==
-
<StructureSection load='6lqk' size='340' side='right'caption='[[6lqk]]' scene=''>
+
<StructureSection load='6lqk' size='340' side='right'caption='[[6lqk]], [[Resolution|resolution]] 2.50&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
-
<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6LQK OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=6LQK FirstGlance]. <br>
+
<table><tr><td colspan='2'>[[6lqk]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Apis_mellifera Apis mellifera]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6LQK OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6LQK FirstGlance]. <br>
-
</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=6lqk FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6lqk OCA], [http://pdbe.org/6lqk PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6lqk RCSB], [http://www.ebi.ac.uk/pdbsum/6lqk PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6lqk ProSAT]</span></td></tr>
+
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.499&#8491;</td></tr>
 +
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
 +
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6lqk FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6lqk OCA], [https://pdbe.org/6lqk PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6lqk RCSB], [https://www.ebi.ac.uk/pdbsum/6lqk PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6lqk ProSAT]</span></td></tr>
</table>
</table>
 +
<div style="background-color:#fffaf0;">
 +
== Publication Abstract from PubMed ==
 +
Ryanodine receptors (RyRs) are the molecular target of diamides, a new chemical class of insecticides. Diamide insecticides are used to control lepidopteran pests and were considered relatively safe for mammals and non-targeted beneficial insects, including honey bees. However, recent studies showed that exposure to diamides could cause long-lasting locomotor deficits of bees. Here we report the crystal structure of RyR N-terminal domain A (NTD-A) from the honeybee, Apis mellifera, at 2.5 A resolution. It shows a similar overall fold as the RyR NTD-A from mammals and the diamondback moth (DBM), Plutella xylostella, and still several loops located at the inter-domain interfaces show insect-specific or bee-specific structural features. A potential insecticide-binding pocket formed by loop9 and loop13 is conserved in lepidopteran but different in both mammals and bees, making it a good candidate targeting site for the development of pest-selective insecticides. Furthermore, a conserved intra-domain disulfide bond was observed in both DBM and bee RyR NTD-A crystal structures, which explains their higher thermal stability compared to mammalian RyR NTD-A. This work provides a basis for the development of novel insecticides with better selectivity between pests and bees by targeting a distinct site on pest RyRs, which would be a promising strategy to overcome the current toxicity problem.
 +
 +
Crystal structure of the N-terminal domain of ryanodine receptor from the honeybee, Apis mellifera.,Zhou Y, Wang W, Salauddin NM, Lin L, You M, You S, Yuchi Z Insect Biochem Mol Biol. 2020 Oct;125:103454. doi: 10.1016/j.ibmb.2020.103454., Epub 2020 Aug 8. PMID:32781205<ref>PMID:32781205</ref>
 +
 +
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
 +
</div>
 +
<div class="pdbe-citations 6lqk" style="background-color:#fffaf0;"></div>
 +
 +
==See Also==
 +
*[[Ryanodine receptor 3D structures|Ryanodine receptor 3D structures]]
 +
== References ==
 +
<references/>
__TOC__
__TOC__
</StructureSection>
</StructureSection>
 +
[[Category: Apis mellifera]]
[[Category: Large Structures]]
[[Category: Large Structures]]
[[Category: Lin L]]
[[Category: Lin L]]
[[Category: Yuchi Z]]
[[Category: Yuchi Z]]
[[Category: Zhou Y]]
[[Category: Zhou Y]]

Revision as of 14:45, 29 November 2023

Crystal structure of honeybee RyR NTD

PDB ID 6lqk

Drag the structure with the mouse to rotate

Proteopedia Page Contributors and Editors (what is this?)

OCA

Personal tools