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| | <StructureSection load='6lvu' size='340' side='right'caption='[[6lvu]], [[Resolution|resolution]] 2.29Å' scene=''> | | <StructureSection load='6lvu' size='340' side='right'caption='[[6lvu]], [[Resolution|resolution]] 2.29Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[6lvu]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Thema Thema]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6LVU OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=6LVU FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[6lvu]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Thermotoga_maritima_MSB8 Thermotoga maritima MSB8]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6LVU OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6LVU FirstGlance]. <br> |
| - | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.294Å</td></tr> |
| - | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">acpP ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=243274 THEMA])</td></tr> | + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=6lvu FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6lvu OCA], [http://pdbe.org/6lvu PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6lvu RCSB], [http://www.ebi.ac.uk/pdbsum/6lvu PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6lvu ProSAT]</span></td></tr> | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6lvu FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6lvu OCA], [https://pdbe.org/6lvu PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6lvu RCSB], [https://www.ebi.ac.uk/pdbsum/6lvu PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6lvu ProSAT]</span></td></tr> |
| | </table> | | </table> |
| | == Function == | | == Function == |
| - | [[http://www.uniprot.org/uniprot/ACP_THEMA ACP_THEMA]] Carrier of the growing fatty acid chain in fatty acid biosynthesis.[HAMAP-Rule:MF_01217] | + | [https://www.uniprot.org/uniprot/ACP_THEMA ACP_THEMA] Carrier of the growing fatty acid chain in fatty acid biosynthesis.[HAMAP-Rule:MF_01217] |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| | == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
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| | </div> | | </div> |
| | <div class="pdbe-citations 6lvu" style="background-color:#fffaf0;"></div> | | <div class="pdbe-citations 6lvu" style="background-color:#fffaf0;"></div> |
| | + | |
| | + | ==See Also== |
| | + | *[[Acyl carrier protein 3D structures|Acyl carrier protein 3D structures]] |
| | == References == | | == References == |
| | <references/> | | <references/> |
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| | </StructureSection> | | </StructureSection> |
| | [[Category: Large Structures]] | | [[Category: Large Structures]] |
| - | [[Category: Thema]] | + | [[Category: Thermotoga maritima MSB8]] |
| - | [[Category: Kim, Y]] | + | [[Category: Kim Y]] |
| - | [[Category: Lee, W C]] | + | [[Category: Lee WC]] |
| - | [[Category: Lee, Y]] | + | [[Category: Lee Y]] |
| - | [[Category: Acyl carrier protein]]
| + | |
| - | [[Category: Biosynthetic protein]]
| + | |
| Structural highlights
Function
ACP_THEMA Carrier of the growing fatty acid chain in fatty acid biosynthesis.[HAMAP-Rule:MF_01217]
Publication Abstract from PubMed
Thermotoga maritima, a deep-branching hyperthermophilic bacterium, expresses an extraordinarily stable Thermotoga maritima acyl carrier protein (Tm-ACP) that functions as a carrier in the fatty acid synthesis system at near-boiling aqueous environments. Here, to understand the hyperthermal adaptation of Tm-ACP, we investigated the structure and dynamics of Tm-ACP by nuclear magnetic resonance (NMR) spectroscopy. The melting temperature of Tm-ACP (101.4 degrees C) far exceeds that of other ACPs, owing to extensive ionic interactions and tight hydrophobic packing. The D59 residue, which replaces Pro/Ser of other ACPs, mediates ionic clustering between helices III and IV. This creates a wide pocket entrance to facilitate the accommodation of long acyl chains required for hyperthermal adaptation of the T. maritima cell membrane. Tm-ACP is revealed to be the first ACP that harbor an amide proton hyperprotected against hydrogen/deuterium exchange for I15. The hydrophobic interactions mediated by I15 appear to be the key driving forces of the global folding process of Tm-ACP. Our findings provide insights into the structural basis of the hyperthermal adaptation of ACP, which might have allowed T. maritima to survive in hot ancient oceans.
Structural Characterization of an ACP from Thermotoga maritima: Insights into Hyperthermal Adaptation.,Lee Y, Jang A, Jeong MC, Park N, Park J, Lee WC, Cheong C, Kim Y Int J Mol Sci. 2020 Apr 9;21(7). pii: ijms21072600. doi: 10.3390/ijms21072600. PMID:32283632[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
See Also
References
- ↑ Lee Y, Jang A, Jeong MC, Park N, Park J, Lee WC, Cheong C, Kim Y. Structural Characterization of an ACP from Thermotoga maritima: Insights into Hyperthermal Adaptation. Int J Mol Sci. 2020 Apr 9;21(7). pii: ijms21072600. doi: 10.3390/ijms21072600. PMID:32283632 doi:http://dx.doi.org/10.3390/ijms21072600
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