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6lx0
From Proteopedia
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==Structure of Leptospira santarosai serovar shermani LRR protein LSS11580== | ==Structure of Leptospira santarosai serovar shermani LRR protein LSS11580== | ||
| - | <StructureSection load='6lx0' size='340' side='right'caption='[[6lx0]]' scene=''> | + | <StructureSection load='6lx0' size='340' side='right'caption='[[6lx0]], [[Resolution|resolution]] 1.99Å' scene=''> |
== Structural highlights == | == Structural highlights == | ||
| - | <table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6LX0 OCA]. For a <b>guided tour on the structure components</b> use [ | + | <table><tr><td colspan='2'>[[6lx0]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Leptospira_santarosai_serovar_Shermani_str._LT_821 Leptospira santarosai serovar Shermani str. LT 821]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6LX0 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6LX0 FirstGlance]. <br> |
| - | </td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[ | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.99Å</td></tr> |
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6lx0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6lx0 OCA], [https://pdbe.org/6lx0 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6lx0 RCSB], [https://www.ebi.ac.uk/pdbsum/6lx0 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6lx0 ProSAT]</span></td></tr> | ||
</table> | </table> | ||
| + | == Function == | ||
| + | [https://www.uniprot.org/uniprot/K8Y7G8_9LEPT K8Y7G8_9LEPT] | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | Leptospirosis is the most common zoonotic disease caused by pathogenic Leptospira, which is classified into three groups according to virulence. Its pathogenic and intermediate species contain leucine-rich repeat (LRR) proteins that are rarely expressed in non-pathogenic strains. In this study, we presented the crystal structure of LSS_11580 (rLRR20) from pathogenic L. santarosai serovar Shermani. X-ray diffraction at a resolution of 1.99 A revealed a horseshoe-shaped structure containing seven alpha-helices and five beta-sheets. Affinity assays indicated that rLRR20 interacts with E-cadherin on the cell surface. Interestingly, its binds to the extracellular (EC) 1 domain in human epithelial (E)-cadherin, which is responsible for binding to another E-cadherin molecule in neighboring cells. Several charged residues on the concave face of LRR20 were predicted to interact with EC1 domain. In the affinity assays, these charged residues were replaced by alanine, and their affinities to E-cadherin were measured. Three vital residues and mutation variants of LRR20, namely D56A, E59A, and E123A, demonstrated significantly reduced affinity to E-cadherin compared with the control. Besides, we also demonstrated that rLRR20 induced the expression of neutrophil gelatinase-associated lipocalin (NGAL) in HK2 cells. The low ability of the three mutation variants to induce NGAL expression further demonstrates this induction. The present findings indicate that LRR20 from pathogenic Leptospira binds to E-cadherin and interacts with its EC1 domain. In addition, its induction of NGAL expression in HK2 cells is associated with acute kidney injury in human. | ||
| + | |||
| + | Crystal structure of Leptospira leucine-rich repeat 20 reveals a novel E-cadherin binding protein to induce NGAL expression in HK2 cells.,Hsu SH, Chu CH, Tian YC, Chang MY, Chou LF, Sun YJ, Yang CW Biochem J. 2020 Nov 13;477(21):4313-4326. doi: 10.1042/BCJ20200547. PMID:33094809<ref>PMID:33094809</ref> | ||
| + | |||
| + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | ||
| + | </div> | ||
| + | <div class="pdbe-citations 6lx0" style="background-color:#fffaf0;"></div> | ||
| + | == References == | ||
| + | <references/> | ||
__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
[[Category: Large Structures]] | [[Category: Large Structures]] | ||
| + | [[Category: Leptospira santarosai serovar Shermani str. LT 821]] | ||
[[Category: Chu CH]] | [[Category: Chu CH]] | ||
[[Category: Hsu SH]] | [[Category: Hsu SH]] | ||
[[Category: Sun YJ]] | [[Category: Sun YJ]] | ||
[[Category: Yang CW]] | [[Category: Yang CW]] | ||
Current revision
Structure of Leptospira santarosai serovar shermani LRR protein LSS11580
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