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| | <StructureSection load='6m0e' size='340' side='right'caption='[[6m0e]], [[Resolution|resolution]] 1.35Å' scene=''> | | <StructureSection load='6m0e' size='340' side='right'caption='[[6m0e]], [[Resolution|resolution]] 1.35Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[6m0e]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Beii9 Beii9]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6M0E OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=6M0E FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[6m0e]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Beijerinckia_indica_subsp._indica_ATCC_9039 Beijerinckia indica subsp. indica ATCC 9039]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6M0E OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6M0E FirstGlance]. <br> |
| - | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=BDF:BETA-D-FRUCTOPYRANOSE'>BDF</scene>, <scene name='pdbligand=FRU:FRUCTOSE'>FRU</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.35Å</td></tr> |
| - | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">Bind_2021 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=395963 BEII9])</td></tr> | + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=BDF:BETA-D-FRUCTOPYRANOSE'>BDF</scene>, <scene name='pdbligand=FRU:FRUCTOSE'>FRU</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr> |
| - | <tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Levansucrase Levansucrase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.4.1.10 2.4.1.10] </span></td></tr> | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6m0e FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6m0e OCA], [https://pdbe.org/6m0e PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6m0e RCSB], [https://www.ebi.ac.uk/pdbsum/6m0e PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6m0e ProSAT]</span></td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=6m0e FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6m0e OCA], [http://pdbe.org/6m0e PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6m0e RCSB], [http://www.ebi.ac.uk/pdbsum/6m0e PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6m0e ProSAT]</span></td></tr> | + | |
| | </table> | | </table> |
| | + | == Function == |
| | + | [https://www.uniprot.org/uniprot/B2IF78_BEII9 B2IF78_BEII9] |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| | == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
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| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Beii9]] | + | [[Category: Beijerinckia indica subsp. indica ATCC 9039]] |
| | [[Category: Large Structures]] | | [[Category: Large Structures]] |
| - | [[Category: Levansucrase]]
| + | [[Category: Tonozuka T]] |
| - | [[Category: Tonozuka, T]] | + | |
| - | [[Category: Fructooligosaccharide]]
| + | |
| - | [[Category: Gh68]]
| + | |
| - | [[Category: Hydrolase]]
| + | |
| Structural highlights
Function
B2IF78_BEII9
Publication Abstract from PubMed
An enzyme belonging to glycoside hydrolase family 68 (GH68) from Beijerinckia indica subsp. indica NBRC 3744 was expressed in Escherichia coli. Biochemical characterization showed that the enzyme was identified to be a beta-fructosyltransferase (BiBftA). Crystallization of a full-length BiBftA was initially attempted, but no crystals were obtained. We constructed a variant in which 5 residues (Pro199-Gly203) and 13 residues (Leu522-Gln534) in potentially flexible regions were deleted, and we successfully crystallized this variant BiBftA. BiBftA is composed of a five-bladed beta-propeller fold as in other GH68 enzymes. The structure of BiBftA in complex with fructose unexpectedly indicated that one beta-fructofuranose (beta-Fruf) molecule and one beta-fructopyranose molecule bind to the catalytic pocket. The orientation of beta-Fruf at subsite -1 is tilted from the orientation observed in most GH68 enzymes, presenting a second structure of a GH68 enzyme in complex with the tilted binding mode of beta-Fruf.
Crystal structure of a glycoside hydrolase family 68 beta-fructosyltransferase from Beijerinckia indica subsp. indica in complex with fructose.,Tonozuka T, Kitamura J, Nagaya M, Kawai R, Nishikawa A, Hirano K, Tamura K, Fujii T, Tochio T Biosci Biotechnol Biochem. 2020 Aug 4:1-13. doi: 10.1080/09168451.2020.1804317. PMID:32752982[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Tonozuka T, Kitamura J, Nagaya M, Kawai R, Nishikawa A, Hirano K, Tamura K, Fujii T, Tochio T. Crystal structure of a glycoside hydrolase family 68 beta-fructosyltransferase from Beijerinckia indica subsp. indica in complex with fructose. Biosci Biotechnol Biochem. 2020 Aug 4:1-13. doi: 10.1080/09168451.2020.1804317. PMID:32752982 doi:http://dx.doi.org/10.1080/09168451.2020.1804317
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