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7bp1

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Current revision (15:23, 29 November 2023) (edit) (undo)
 
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==Crystal structure of 2, 3-dihydroxybenzoic acid decarboxylase from Fusarium oxysporum in complex with Catechol==
==Crystal structure of 2, 3-dihydroxybenzoic acid decarboxylase from Fusarium oxysporum in complex with Catechol==
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<StructureSection load='7bp1' size='340' side='right'caption='[[7bp1]]' scene=''>
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<StructureSection load='7bp1' size='340' side='right'caption='[[7bp1]], [[Resolution|resolution]] 1.97&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
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<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7BP1 OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=7BP1 FirstGlance]. <br>
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<table><tr><td colspan='2'>[[7bp1]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Fusarium_oxysporum Fusarium oxysporum]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7BP1 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7BP1 FirstGlance]. <br>
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</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=7bp1 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7bp1 OCA], [http://pdbe.org/7bp1 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=7bp1 RCSB], [http://www.ebi.ac.uk/pdbsum/7bp1 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=7bp1 ProSAT]</span></td></tr>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.97&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CAQ:CATECHOL'>CAQ</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7bp1 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7bp1 OCA], [https://pdbe.org/7bp1 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7bp1 RCSB], [https://www.ebi.ac.uk/pdbsum/7bp1 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7bp1 ProSAT]</span></td></tr>
</table>
</table>
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== Function ==
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[https://www.uniprot.org/uniprot/N1S495_FUSC4 N1S495_FUSC4]
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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A 2,3-dihydroxybenzoic acid decarboxylase from Fusarium oxysporum (2,3-DHBD_Fo) has a relatively high catalytic efficiency for the decarboxylation of 2,3-dihydroxybenzoic acid (DHBA) and carboxylation of catechol, thus it has a different substrate spectrum from other benzoic acid decarboxylases. We have determined the structures of 2,3-DHBD_Fo in its apo form and complexes with catechol or 2,5-dihydroxybenzoic acid at 1.55, 1.97, and 2.45 A resolution, respectively. The crystal structures of 2,3-DHBD_Fo show that the enzyme exists as a homotetramer, and each active center has a Zn(2+) ion coordinated by E8, H167, D291 and three water molecules. This is different from 2,6-DHBD from Rhizobium sporomusa, in which the Zn(2+) ion is also coordinated with H10. Surprisingly, mutation of A10 of 2,3-DHBD_Fo to His resulted in almost complete loss of the enzyme activity. Enzyme-substrate docking and site-directed mutation studies indicate that residue R233(Delta) interacts with the 3-hydroxy group of 2,3-DHBA, and plays an important role in substrate recognition for this enzyme, thus revealing the molecular basis 2,3-dihydroxybenzoic acid decarboxylase.
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2,3-Dihydroxybenzoic Acid Decarboxylase from Fusarium oxysporum: Crystal Structures and Substrate Recognition Mechanism.,Song M, Zhang X, Liu W, Feng J, Cui Y, Yao P, Wang M, Guo RT, Wu Q, Zhu D Chembiochem. 2020 Oct 15;21(20):2950-2956. doi: 10.1002/cbic.202000244. Epub 2020, Jul 2. PMID:32421914<ref>PMID:32421914</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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<div class="pdbe-citations 7bp1" style="background-color:#fffaf0;"></div>
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== References ==
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<references/>
__TOC__
__TOC__
</StructureSection>
</StructureSection>
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[[Category: Fusarium oxysporum]]
[[Category: Large Structures]]
[[Category: Large Structures]]
[[Category: Feng JH]]
[[Category: Feng JH]]

Current revision

Crystal structure of 2, 3-dihydroxybenzoic acid decarboxylase from Fusarium oxysporum in complex with Catechol

PDB ID 7bp1

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