7bwk

From Proteopedia

(Difference between revisions)

Current revision

Structure of DotL(656-783)-IcmS-IcmW-LvgA-VpdB(461-590) derived from Legionella pneumophila

Structural highlights

7bwk is a 10 chain structure with sequence from Legionella pneumophila subsp. pneumophila str. Philadelphia 1. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:	X-ray diffraction, Resolution 2.801Å
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

DOTL_LEGPH Component of the Dot/Icm type IVB secretion system (T4BSS), which is used to inject bacterial effector proteins into eukaryotic host cells (PubMed:17040490, PubMed:22694730, PubMed:32513920). Part of a subcomplex which recruits effector proteins and delivers them to the core transmembrane subcomplex (PubMed:23028312, PubMed:32513920). Plays a central role in the assembly of the subcomplex (PubMed:32513920). Required for the recruitment of IcmS and IcmW to the inner membrane and for the translocation of adapter-dependent substrates (PubMed:23028312). May have ATPase activity (Probable).^[1] ^[2] ^[3] ^[4] ^[5]

Publication Abstract from PubMed

The Legionella pneumophila Dot/Icm type IVB secretion system (T4BSS) is extremely versatile, translocating ~300 effector proteins into host cells. This specialized secretion system employs the Dot/Icm type IVB coupling protein (T4CP) complex, which includes IcmS, IcmW and LvgA, that are known to selectively assist the export of a subclass of effectors. Herein, the crystal structure of a four-subunit T4CP subcomplex bound to the effector protein VpdB reveals an interaction between LvgA and a linear motif in the C-terminus of VpdB. The same binding interface of LvgA also interacts with the C-terminal region of three additional effectors, SidH, SetA and PieA. Mutational analyses identified a FxxxLxxxK binding motif that is shared by VpdB and SidH, but not by SetA and PieA, showing that LvgA recognizes more than one type of binding motif. Together, this work provides a structural basis for how the Dot/Icm T4CP complex recognizes effectors, and highlights the multiple substrate-binding specificities of its adaptor subunit.

Structural basis for effector protein recognition by the Dot/Icm Type IVB coupling protein complex.,Kim H, Kubori T, Yamazaki K, Kwak MJ, Park SY, Nagai H, Vogel JP, Oh BH Nat Commun. 2020 May 26;11(1):2623. doi: 10.1038/s41467-020-16397-0. PMID:32457311^[6]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Vincent CD, Friedman JR, Jeong KC, Buford EC, Miller JL, Vogel JP. Identification of the core transmembrane complex of the Legionella Dot/Icm type IV secretion system. Mol Microbiol. 2006 Dec;62(5):1278-91. doi: 10.1111/j.1365-2958.2006.05446.x. , Epub 2006 Oct 16. PMID:17040490 doi:http://dx.doi.org/10.1111/j.1365-2958.2006.05446.x
↑ Vincent CD, Friedman JR, Jeong KC, Sutherland MC, Vogel JP. Identification of the DotL coupling protein subcomplex of the Legionella Dot/Icm type IV secretion system. Mol Microbiol. 2012 Jul;85(2):378-91. doi: 10.1111/j.1365-2958.2012.08118.x. Epub , 2012 Jun 14. PMID:22694730 doi:http://dx.doi.org/10.1111/j.1365-2958.2012.08118.x
↑ Sutherland MC, Nguyen TL, Tseng V, Vogel JP. The Legionella IcmSW complex directly interacts with DotL to mediate translocation of adaptor-dependent substrates. PLoS Pathog. 2012 Sep;8(9):e1002910. doi: 10.1371/journal.ppat.1002910. Epub 2012 , Sep 13. PMID:23028312 doi:http://dx.doi.org/10.1371/journal.ppat.1002910
↑ Meir A, Mace K, Lukoyanova N, Chetrit D, Hospenthal MK, Redzej A, Roy C, Waksman G. Mechanism of effector capture and delivery by the type IV secretion system from Legionella pneumophila. Nat Commun. 2020 Jun 8;11(1):2864. doi: 10.1038/s41467-020-16681-z. PMID:32513920 doi:http://dx.doi.org/10.1038/s41467-020-16681-z
↑ Meir A, Mace K, Lukoyanova N, Chetrit D, Hospenthal MK, Redzej A, Roy C, Waksman G. Mechanism of effector capture and delivery by the type IV secretion system from Legionella pneumophila. Nat Commun. 2020 Jun 8;11(1):2864. doi: 10.1038/s41467-020-16681-z. PMID:32513920 doi:http://dx.doi.org/10.1038/s41467-020-16681-z
↑ Kim H, Kubori T, Yamazaki K, Kwak MJ, Park SY, Nagai H, Vogel JP, Oh BH. Structural basis for effector protein recognition by the Dot/Icm Type IVB coupling protein complex. Nat Commun. 2020 May 26;11(1):2623. doi: 10.1038/s41467-020-16397-0. PMID:32457311 doi:http://dx.doi.org/10.1038/s41467-020-16397-0

1 Structural highlights
2 Function
3 Publication Abstract from PubMed
4 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/7bwk"

Categories: Large Structures | Legionella pneumophila subsp. pneumophila str. Philadelphia 1 | Kim H | Kwak MJ | Oh BH

@@ Line 3: / Line 3: @@
 <StructureSection load='7bwk' size='340' side='right'caption='[[7bwk]], [[Resolution|resolution]] 2.80&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[7bwk]] is a 10 chain structure with sequence from [https://en.wikipedia.org/wiki/Legph Legph]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7BWK OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7BWK FirstGlance]. <br>
+<table><tr><td colspan='2'>[[7bwk]] is a 10 chain structure with sequence from [https://en.wikipedia.org/wiki/Legionella_pneumophila_subsp._pneumophila_str._Philadelphia_1 Legionella pneumophila subsp. pneumophila str. Philadelphia 1]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7BWK OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7BWK FirstGlance]. <br>
-</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">icmO, lpg0446 ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=272624 LEGPH]), icmS, lpg0442 ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=272624 LEGPH]), icmW, lpg2688 ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=272624 LEGPH]), lpg0525 ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=272624 LEGPH]), lpg1227 ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=272624 LEGPH])</td></tr>
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.801&#8491;</td></tr>
 <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7bwk FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7bwk OCA], [https://pdbe.org/7bwk PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7bwk RCSB], [https://www.ebi.ac.uk/pdbsum/7bwk PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7bwk ProSAT]</span></td></tr>
 </table>
+== Function ==
+[https://www.uniprot.org/uniprot/DOTL_LEGPH DOTL_LEGPH] Component of the Dot/Icm type IVB secretion system (T4BSS), which is used to inject bacterial effector proteins into eukaryotic host cells (PubMed:17040490, PubMed:22694730, PubMed:32513920). Part of a subcomplex which recruits effector proteins and delivers them to the core transmembrane subcomplex (PubMed:23028312, PubMed:32513920). Plays a central role in the assembly of the subcomplex (PubMed:32513920). Required for the recruitment of IcmS and IcmW to the inner membrane and for the translocation of adapter-dependent substrates (PubMed:23028312). May have ATPase activity (Probable).<ref>PMID:17040490</ref> <ref>PMID:22694730</ref> <ref>PMID:23028312</ref> <ref>PMID:32513920</ref> <ref>PMID:32513920</ref>
 <div style="background-color:#fffaf0;">
 == Publication Abstract from PubMed ==
@@ Line 21: / Line 23: @@
 </StructureSection>
 [[Category: Large Structures]]
-[[Category: Legph]]
+[[Category: Legionella pneumophila subsp. pneumophila str. Philadelphia 1]]
-[[Category: Kim, H]]
+[[Category: Kim H]]
-[[Category: Kwak, M J]]
+[[Category: Kwak MJ]]
-[[Category: Oh, B H]]
+[[Category: Oh BH]]
-[[Category: Dot/icm type 4b secretion system]]
-[[Category: Dotl-icmsw-lvga]]
-[[Category: Effector recognition]]
-[[Category: Protein transport]]
-[[Category: Type 4b coupling protein complex-effector complex]]
-[[Category: Vpdb]]

7bwk

From Proteopedia

Current revision

Structure of DotL(656-783)-IcmS-IcmW-LvgA-VpdB(461-590) derived from Legionella pneumophila

Structural highlights

Function

Publication Abstract from PubMed

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

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